关键词: 乌头注射液, 抗炎, 大鼠, Wistar, 小鼠, ICR

Abstract:

Objective To investigate the effect of Wutou Injection on a variety of different stages of inflammation models, and to initially clarify its anti-inflammatory mechanism. Methods The Wistar rats were randomly divided into model group, low, medium, and high doses (0.4, 0.8, 1.6 mg·kg^-1) of Wutou Injection groups, and positive control group (etanercept, 10 mg·kg^-1);the rats in model group and positive control group at the 1st and 4th day were administered subcutaneously;the rats in Wutou injection groups were intramuscularly administered for 7 consecutive days.The ICR mice were randomly divided into blank control group, low, medium and high doses (0.5, 1.0, 2.0 mg·kg^-1) of Wutou Injection groups, and positive control group (etanercept, 14 mg·kg^-1);the mice in model group and positive control group at the 1st and 4th day were administered subcutaneously;the mice in Wutou Injection groups were intramuscularly administered for 7 consecutive days.The acute and chronic animal models, such as carrageenan-induced paw oedema in the rats, formaldehyde-induced paw oedema in the rats, CMC-Na induced air pouch leukocyte migration in the rats, granuloma induced by cotton ball in the rats, the permeability of blood capillary in abdominal cavity of the mice, were selected to evaluate the paw swelling rates, the number of leukocytes from subcutaneous CMC-Na cysts, organ coefficient, and permeabilities of blood capillary in abdominal cavity. Results Compared with model control group, the paw swelling rates of the rats in medium and high doses of Wutou Injection groups were decreased 1, 4, 5 and 6 h after inflammation induced by carrageenan (P<0.05 or P<0.01); the paw swelling rates of the rats in medium and high doses of Wutou Injection groups were decreased from 6 to 48 h after inflammation induced by formaldehyde (P<0.05 or P<0.01);the number of leukocytes from subcutaneous CMC-Na cysts of the rats in high dose of Wutou Injection group was decreased (P<0.05); the coefficient of granuloma induced by cotton ball of the rats in low, medium and high doses of Wutou Injection groups were decreased (P<0.05 or P<0.01);and the organ indexes had no changes(P>0.05).Compared with blank control group, the permeabilities of blood capillary in abdominal cavity of the mice in low, medium, and high doses of Wutou Injection groups had no significant changes (P>0.05). Conclusion Wutou Injection has anti-inflammatory effects on the different stages of inflammation animal models.

Key words: Wutou Injection, anti-inflammatory, rats, Wistar, mice