关键词: ω-6多不饱和脂肪酸, 降血脂, 实验, 动物

Abstract: Objective To observe the action of lowering blood fat of Agaricus blazei Murill ω-6 polyunsaturated fatty acid(ω-6APFA) on hyperlipemia rats and mice. Methods Sixty rats were randomly divided into control group, model group, natural soybean phospholipids capsule (NSPC)0.70 g•kg^-1 group,ω-6APFA 0.28, 0.14, 0.07 g •kg^-1 groups(n=10). The other groups except control group were given high-fat diets for 14 d, on the fourteenth day the rats were administered orally, the control group and model group were administered distilled water 10mL•kg^-1 at the same volume , 14 days after continuous administration ,rats were anesthetized , the blood were extracted from abdominal artery ，T-CHO, TG, HDL-C, LDL-C in sera were determine d. At the same time, the activity of SOD in liver and the content of MDA were determined, the fat accumulated coefficient was calculated. 72 male mice were randomly divided into control group , model group, NSPC 1.0 g•kg^-1 group,ω-6APFA 0.4, 0.2, 0.1 g•kg^-1 groups(n=12) . Mice were administered continuously,16 h before the last administration ,except control group, the mice in the other groups were injected 75% yolk physiological salt solution 0.5 mL through the abdominal cavity, and began to starve ,1 h after the last administration, blood was extracted from eyeball , serum T-CHO and TG were determined. Results Compared with model group, T-CHO and TG in rats treated with NSPC 0.70 g•kg^-1 and ω-6APFA 0.28 g•kg^-1 all reduced and HDL-C raised obviously(P<0.001), the activities of liver SOD in rats treated with NSPC 0.70 g•kg^-1 and ω-6APFA 0.28,0.14 g• kg^-1 obviously raised (P<0.05, P<0.001, P<0.05), MDA reduced obviously(P<0.01,P<0.001，P<0.01), fat accumulation coefficient in rats treated with NSPC 0.70 g•kg^-1 and ω-6APFA reduced obviously (P>0.01). Compared with model mice , T-CHO and TG in acute hyperlipemia mice treated with NSPC 1.0 g•kg^-1 and ω-6APFA0.4,0.2 g •kg^-1 reduced obviously(P<0.05, P<0.01,P<0.05).Conclusion ω-6APFA has the function of lowering blood fat on hyperlipemia rats and mice.

Key words: ω-6 polyunsaturated fatty acid, lowering blood fat, experiment, animal