关键词: 抗肿瘤, 免疫调节, 动物, 实验

Abstract: Objective To study the anti-tumor activity of Phellinus linteus(PL) and itsimmunomodulational effects. Methods Solid tumor was induced by injecting HepA, MFC, S₁₈₀ cells (310⁶cells/animal) subcutaneously to the right anterior limb in mice. The tumor weight, thymus weight and spleen weight were measured after mice were executed. The effects of PL on lymphocyte proliferation, mitogen-induced proliferatoin of ICR mouse splenocytes were measured by MTT. Meanwhile, lymphocytes were prepared as described above. SRBC (10%, 0.2 mL) was injected to abdomina l cavity to elevate the level of antibody production by B cells. Spleen cells suspension (1×10⁷•mL^-1) was used as the effect cells and L₉₂₉ cells was used as the target cells. L₉₂₉ cells engulfing neutral red determined the cytotoxic act ivity of NK. Simultaneously, phagocytosis of mice was detected using the method that macrophages engulfed neutral red. The effect of PL on the production of antibody was studied, the lymphocytes from mice wereprepared as described above. Results The high-dose (2.0 g•kg^-1) and mid-dose (1.0 g•kg^-1) of PL could significantly inhibit the growth of HepA, MFC and S₁₈₀tumor, the inhibitory rates were 38.19% and 37.15%,32. 46% and 30.91%,36.0% and 34.16%, respectively (P<0.05, P<0.01). The average absorbanc e of lymphocytes in 2.0 g•kg^-1 group was higher than that in the untreated control group. The numbers of antibody forming cells were increased by PL treatment at doses of 2.0 g•kg^-1 and 1.0 g•kg^-1 (P<0.05, P<0.001), the difference was significant compare d with control group (P<0.05). PL (2.0 g•kg^-1) enhanced the cytoto xic activity of NK cells (P<0.01). Average weights of the spleens and thymus (0.1 mg per gram body weight) were not significantly greater than those in positive control groups . PL(2.0 and 1.0 g•kg^-1) increased the activity of macrophage (P<0.05, P<0.01). Conclusion PL has anti-tumor activity and immunomodulational effect and probably inhibit tumor by means of activating the immunocompetence of the body.

Key words: anti-tumor, immunomodutation, animals, laboratory