关键词: 成纤维细胞, 成骨细胞, 酶联免疫吸附测定, 逆转录聚合酶链反应

Abstract: To explore the effects of fluoride with different doses on the expressions of TGF-β and Smad2/3 in fibroblast and osteoblast at different periods. Methods Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0，0.0001，0.001，0.1，1，10 and 20 mg•L^-1) . The levels of TGF-β protein and Smad2/3 at 2,4,24,48 and 72 h after treatment were measured by using ELISA method. The expression of TGF-β mRNA was tested with RT-PCR method. Results In fibroblasts，the contents of TGF-β protein were decreased in the groups of 0.001，0.1，1，10 and 20 mg•L^-1 F^- a t the time of 2 h and in the groups of 0.0001，0.001，0.1，10 and 20mg•L¹ at the time of 4 h（P<0.01，P<0.05). Thelevels of TGF-β mRNA were increased in the groups of 1 and 20 mg•L^-1F^- at 48 h and the expressions of Smad2/3 were increased in fluoride groups during 2 to 24 h. Inosteoblasts，the expressions of TGF-βprotein and mRNA were increased significantly in the groups of 0.0001，0.001 and 1 mg•L-1 F^-at 48 h（P<0.01，P<0.05)and the level of Smad2/3 elevated in the group of 20 mg•L^-1 F^- at 48 h（P<0.01)；Conclusion TGF-β may play roles in the develop ing of osteogenesitic function in fibroblasts induced by fluoride.