关键词: 人T淋巴细胞白血病细胞株, 小鼠T淋巴瘤细胞株, 细胞周期

Abstract: Abstract:Objective To investigate the effect of X-irradiation on cell cycle progression of Jurkat and EL-4 cell lines. Methods The time- and dose-effects of cell cycle progression in Jurkat and EL-4 lymphoma cell lines after X-irradiation with different doses (1.0,2.0 and 4.0 Gy) were analyzed by flow cytometry (FCM) following staining with propidium iodide (PI). Results In time-effect experiment,it was demonstrated that the percentages of Jurkat cells in G₀/G₁ and S phases were decreased significantly after 2.0 Gy X-irradiation（P<0.01）,whereas G₂+M phases cells were increased significantly at the same time compared with sham-irradiation（P<0.01）.It was also showed that the percentages of EL-4 cells in G₂+M and G₀/G₁ phases were increased（P<0.05,P<0.01）,and S phase cells were decreased significantly（P<0.01）. For dose-effect experiment,the results showed that the percentages of Jurkat cells in G₀/G₁ and S phases were decreased significantly 16 h after X-irradiation with the doses of 1.0,2.0 and 4.0 Gy（P<0.05,P<0.01）,and G₂+M phases cells were increased compared with sham-irradiation（P<0.01）. The percentage of EL-4 cells in G₀/G₁ phase was increased（P<0.01）,and S phase cells were decreased（P<0.01）.However,the percentage of EL-4 cells in G₂+M phases was increased significantly only after 4.0 Gy X-irradiation（P<0.01）. Conclusion The cell cycle progression of Jurkat and EL-4 lymphoma cell lines could be changed by X-irradiation with the doses of 1.0,2.0 and 4.0 Gy. X-irradiation（1.0,2.0 and 4.0 Gy ）could induce G₂ arrest in Jurkat cells,G₂ and G₁ arrest in EL-4 cells.

Key words: Jurkat cells, EL-4 cells, cell cycle