关键词: 非小细胞肺, 遗传学, 基因, p53, 辐射效应, 疾病遗传易感性, 流行病学, 辐射耐受性, 遗传学

Abstract: Objective To study the association of polymorphism in TP53 gene with the susceptibility and radiation sensitivity of non-small-cell-lung cancer (NSCLC) of the population in the North of China. Methods Using RFLP-PCR assays, TP53 genotypes were detected by amplifying DNA fragments with sequence specific primers and digested by FnuDⅡ enzyme in 88 patients with NSCLC as well as 112 healthy controls. Results The C/C allele frequency was significantly higher in NSCLC patients than that in the healthy controls (χ²=5.65,P=0.017) . The C/C genotype frequency was significantly higher in NSCLC patients than that of the healthy controls (χ²=9.33,P=0.0023). The risk of C/C homozygotes in NSCLC patients was about 2.7 times against G/G homozygotes with odds ratio of 2.43 (95% CI=1.32-4.51). Conclusion In the population in the North of China, TP53 C/C genotype is closely associated with the susceptibility of NSCLC. There is no significant relationship between the polymorphism in TP53 gene and radiation sensitivity in NSCLC.

Key words: non-small-cell lung, genetics, genes, p53, radiation effects, genetic predisposition to disease, epidemiology, radiation tolerance, genetics