关键词: 遗传学, 基因, p53, 基因, Bcl-2, Bax, 内皮生长因子, 遗传学, 新生血管密度

Abstract: Objective To study the expressions of p16, Bcl-2, Bax, VEGF and significance of microvessel density(MVD) in human pancreatic cancer. Methods Immunohistochemistry method and morphological semi-quantitative analysis were used to detect the quantity of MVD and the expressions of p16,Bcl-2,Bax, and VEGF in 33 cases of pancreatic cancer and 6 normal pancreas tissue specimen. Results The expression of P16 protein: it was higher in moderately or well differentiated pancreatic adenocarcinoma than poorly one (P<0.05), it was higher in patient with long survial time than those with short survial time (P<0.05);the expression of Bcl-2: it was higher in moderately or well differentiated pancreatic adenocarcinoma than poorly one; the expression of Bax:it was higher in normal pancreatic tissue than pancreatic cancer, as well as moderately or well differentiated than poorly one and survival time≥12 months than <12 months; the expression of VEGF:it was higher in patients with lymph node metastasis than without lymph node metastasis; MVD count: it was lower in normal pancreatic tissue than pancreatic cancer as well as with lymph node metastasis than without lymph node metastasis; the expression of MVD had positive correlation with VEGF expression （r=0.79，P<0.05）；p16 gene expression had negative correlation with VEGF expression and MVD （r=-0.69,P<0.05;r=-0.87,P<0.05），but Bcl-2expression had no correlation with other gene expressions. Bax expression had positive correlation with p16 gene expresion （r=0.54,P<0.05），and had negative correlation with VEGF expression and MVD（r=-0.66,P<0.05）. Conclusion The determination of p16, Bcl-2, Bax, VEGF expressions and MVD in human pancreatic cancer may be used to evaluate the prognosis and judge the differentiation of the adenocarcinoma.

Key words: genetics, genes, p53, genes, Bcl-2, Bax, endothelial growth factors, genetics, microvessel density