吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

单宁酸对高糖和糖化终末产物培养条件下肾小球系膜细胞氧化应激及微炎症状态的改善作用

魏海峰1,李才2,方艳秋1,魏雁虹3,谭岩1   

  1. 1.吉林省人民医院中心实验室,吉林 长春 130021;2.吉林大学转化医学院,吉林 长春 130021;3.吉林省人民医院检验科,吉林 长春 130021
  • 收稿日期:2013-11-14 出版日期:2014-09-28 发布日期:2014-11-24
  • 通讯作者: 谭 岩(Tel:0431-85595141,E-mail:tanyan49@hotmail.com) E-mail:tanyan49@hotmail.com
  • 作者简介: 魏海峰(1978-),男,吉林省长春市人,医学博士,主要从事糖尿病及肿瘤分子病理学基础与临床研究。
  • 基金资助:

    吉林省科技厅重点实验室项目资助课题(20122113)

Ameliorating effect of tannic acid on high glucose or AGEs induced oxidative stress and micro-inflammatory state in glomerular mesangial cells

WEI Hai-feng1, LI Cai2, FANG Yan-qiu1, WEI Yan-hong3, TAN Yan1   

  1. 1. Central Laboratory, People’s Hospital, Jilin Province, Changchun 130021, China;2. Institute for Translational Medicine, Jilin University, Changchun 130021, China;3. Department of  Laboratory, People’s Hospital,Jilin Province, Changchun 130021, China
  • Received:2013-11-14 Online:2014-09-28 Published:2014-11-24

摘要:

目的:探讨单宁酸对肾小球系膜细胞(GMC)的作用,从氧化应激及微炎症角度阐明单宁酸改善糖尿病肾病(DN)的作用机制。方法:体外培养GMC,分别用高浓度葡萄糖(30 mmol/L)及糖化终末产物(AGEs)牛血清白蛋白(BSA,250 mg/L)处理,正常糖培养及不含糖的BSA处理的细胞作为相应对照组,在高糖或AGEs处理的同时采用不同浓度单宁酸(10、20、40和80 μmol/L)进行干预,培养48 h后检测细胞培养上清中丙二醛(MDA)水平及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,ELISA法检测细胞培养上清中8-羟基脱氧鸟苷(8-OHdG)水平,免疫组织化学法检测GMC中细胞间黏附分子1(ICAM-1)蛋白表达,RT-PCR法检测GMC中单核细胞趋化蛋白1(MCP-1)和ICAM-1 mRNA的表达水平。结果:与高糖组和AGEs组比较,单宁酸处理组MDA水平明显降低(P<0.05),GSH-Px、SOD及CAT活性明显升高(P<0.05或P<0.01),GMC培养上清中8-OHdG水平明显降低(P<0.05)。与高糖组和AGEs组比较,40 和80 μmol/L单宁酸组ICAM-1蛋白表达水平明显降低(P<0.05)。与高糖组比较,单宁酸组MCP-1和ICAM-1 mRNA表达水平明显降低(P<0.01)。结论:单宁酸可保护GMC免受氧化及炎症介质的损伤,从而延缓和改善DN的肾小球病变。

关键词: 单宁酸, 糖尿病肾病, 氧化应激, 微炎症, 8-羟基脱氧鸟苷

Abstract:

Abstract:Objective To investigate the effect of tannic acid on glomerular mesangial cells (GMC), and to clarify the mechanism of tannic acid in improving the pathological changes of diabetic nephropathy (DN) from the aspect of oxidative stress and micro-inflammation. Methods   The glomerular mesangial cells were treated with glucose (30 mmol/L) or  advanced glycosylation end-products (AGEs) bovine serum albumin(BSA) (250 mg/L) and then different concentrations of tannic acid (10, 20, 40 and 80 μmol/L) were added into the GMC. The cells cultured by normal glucose or treated with BSA were used as control groups and then the level of malonic dialdehyde(MDA), glutathione peroxidase (GSH-Px ), superoxide Dismutase(SOD), CAT (Catalase) activities and   8-hydroxy-2′-deoxyguanosine(8-OHdG) levels in the culture supernatant 48 h after culture  were determined by colorimetry and ELISA method. The expressions of  intercellular cell adhesion molecule-1(ICAM-1)  protein,monocyte chemotactic protein 1(MCP-1) and  ICAM-1 mRNA in GMC  were detected by immunohistochemical staining and RT-PCR method. Results  Compared with high glucose and AGEs groups, the MDA levels in  tannic acid groups were reduced significantly(P<0.05); the activities of GSH-Px,  SOD and CAT were increased significantly(P<0.05 or P<0.01);the 8-OHdG levels in annic acid groups were significantly reduced (P<0.05). Compared with  high glucose and AGEs groups, the expressions levels of ICAM-1  protein in 40 and 80 μmol/L tannic acid  groups were decreased(P<0.05). The mRNA expressions levels  of MCP-1 and ICAM-1 were significantly lower  than those in  high glucose group (P<0.01).  Conclusion  Tannic acid could protect GMC against the damage of oxidative and inflammatory mediators, thereby delaying and improving the glomerular lesions of DN.

Key words:  tannic acid, diabetic nephropathy, oxidative stress, micro-inflammation, 8-hydroxy-2'-deoxyguanosine

中图分类号: 

  • R-332