NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20160219

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (02): 290-294.doi: 10.13481/j.1671-587x.20160219

NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义

金霖霖¹, 邵立虹¹, 魏威¹, 董卓¹, 袁天阳¹, 高辉¹, 韩海玲¹, 从宪玲², 金顺子¹

1. 吉林大学公共卫生学院卫生部放射生物学重点实验室, 吉林长春 130021;
2. 吉林大学中日联谊医院组织标本库, 吉林长春 130033

收稿日期:2015-07-21 发布日期:2016-03-31
通讯作者: 金顺子,教授,博士研究生导师(Tel:0431-85619443,E-mail:jinsz@jlu.edu.cn);丛宪玲,教授,硕士研究生导师(Tel:0431-89876626,E-mail:1440534286@qq.com) E-mail:jinsz@jlu.edu.cn;1440534286@qq.com
作者简介:金霖霖(1989-),女,内蒙古自治区乌兰浩特市人,在读医学硕士,主要从事辐射肿瘤学方面的研究。
基金资助:
国家自然科学基金资助课题(30870584,81371890)

Expressions of miR-9 and NRP1 in human NSCLC tissue and adjacent carcinoma tissueand their clinical significances

JIN Linlin¹, SHAO Lihong¹, WEI Wei¹, DONG Zhuo¹, YUAN Tianyang¹, GAO Hui¹, HAN Hailing¹, CONG Xianling², JIN Shunzi¹

1. Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;
2. Tissue Samples Library, China-Japan Union Hospital, Jilin University, Changchun 130033, China

Received:2015-07-21 Published:2016-03-31

摘要/Abstract

摘要：

目的: 检测人非小细胞肺癌(NSCLC)组织标本中神经纤毛蛋白1(NRP1)mRNA和miR-9的表达水平,并探讨二者表达与NSCLC患者临床特征之间的关系。方法: 在患者知情同意下,收集2010-2011年吉林大学中日联谊医院匹配的NSCLC患者组织标本,包括45例正常组织、45例癌旁组织及45例肿瘤组织。采用qRT-PCR法检测不同组织中NRP1 mRNA和miR-9表达水平,分析二者表达水平与不同临床病理特征的相关性。结果: 与正常组织比较,癌旁组织中NRP1 mRNA表达水平无明显变化(P>0.05),而肿瘤组织中NRP1 mRNA表达水平明显降低(P<0.05);与正常组织比较,癌旁组织miR-9表达水平无明显变化(P>0.05),而肿瘤组织中miR-9表达水平明显升高(P<0.05)。男性癌旁组织中miR-9表达水平低于女性(P<0.05)。NSCLC患者肿瘤组织中NRP1 mRNA表达水平与性别、年龄、分化程度、TNM分期及临床分期无关联(P>0.05),但与病理分型及淋巴结转移有关联(P<0.05);NSCLC患者肿瘤组织中miR-9表达水平与年龄、肿瘤病理类型、淋巴转移、分化程度、TNM分期及临床分期无关联(P>0.05),而与性别因素有关联(P<0.05)。结论: NSCLC患者肿瘤组织中miR-9表达水平显著升高,而NRP1 mRNA表达水平明显下降,NRP1 mRNA检测有助于判断NSCLC的分型及转移。

关键词: 神经纤毛蛋白1, miR-9, 癌, 非小细胞肺, 临床病理特征

Abstract:

Objective: To detect the expression levels of neuropilin1(NRP1) mRNA and miR-9 in non-small cell lung cancer (NSCLC) tissue samples, and to explore the correlations between the expressions of NRP1 mRNA, miR-9 and the clinicopathological characteristics of the patients with NSCLC. Methods: Informed consent was obtained from each patient before surgery.The tissue samples including 45 NSCLC tissue, 45 adjacent carcinoma tissue and 45 normal lung tissue were collected from China-Japan Union Hospital of Jilin University from 2010 to 2011. qRT-PCR was used to detect the expression levels of NRP1 mRNA and miR-9 in three kinds of lung tissue, and the correlation between the expressions of NRP1 mRNA, miR-9 and clinicopathological characteristics of the patients with NSCLC was analyzed.Results: Compared with normal tissue, the expression level of NRP1 mRNA in adjacent carcinoma tissue had no change(P>0.05), but the expression level of NRP1 mRNA in non-small cell lung cancer tissue was significantly decreased (P<0.05).Compared with normal tissue, the expression level of miR-9 in adjacent carcinoma tissue had no change(P>0.05), but the expression level of miR-9 in non-small cell lung cancer tissue was significantly increased (P<0.05).Furthermore, in adjacent carcinoma tissue, the expression level of miR-9 in the males was lower than that in the females(P<0.05).In NSCLC tissue, the expression level of NRP1 mRNA had no relationship with sex, age, differentiation degree, TNM stage and clinical stage, but was significantly correlated to the histological subtype and lymph node metastasis (P<0.05). In NSCLC tissue, the expression level of miR-9 had no relationship with age, pathological type, lymph node metastasis, differentiation degree, TNM stage, and clinical stage (P>0.05), but was correlated to the sex(P<0.05).Conclusion: The expression level of miR-9 is up-regulated and the expression level of NRP1 mRNA is down-regulated significantly in non-small cell lung cancer tissue.The detection of the expression level of NRP1 mRNA contributes to judge the histological subtype and lymph node metastasis of NSCLC.

Key words: neuropilin1, miR-9, carcinoma, non-small cell lung, clinicopathological characteristics

中图分类号:

R734.2

金霖霖, 邵立虹, 魏威, 董卓, 袁天阳, 高辉, 韩海玲, 从宪玲, 金顺子. NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(02): 290-294.

JIN Linlin, SHAO Lihong, WEI Wei, DONG Zhuo, YUAN Tianyang, GAO Hui, HAN Hailing, CONG Xianling, JIN Shunzi. Expressions of miR-9 and NRP1 in human NSCLC tissue and adjacent carcinoma tissueand their clinical significances[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 290-294.

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NRP1和miR-9在人NSCLC组织和癌旁组织中的表达及其临床意义

Expressions of miR-9 and NRP1 in human NSCLC tissue and adjacent carcinoma tissueand their clinical significances

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