支气管舒张剂治疗无效的咳嗽变异性哮喘患者炎性介质水平和炎性细胞百分比的检测及其意义

doi:10.13481/j.1671-587x.20160225

摘要/Abstract

摘要：

目的: 检测咳嗽变异性哮喘(CVA)患者炎性介质水平和炎性细胞百分比,阐明白细胞介素8(IL-8)和中性粒细胞在支气管舒张剂治疗无效的CVA发病中的作用。方法: 随机选择60例CVA患者,分为支气管舒张剂治疗无效组(n=30)和支气管舒张剂治疗有效组(n=30),同时选取健康人群作为正常对照组(n=30)。检测3组研究对象诱导痰上清液中IL-8水平和嗜酸性粒细胞阳离子蛋白(ECP)水平,观察诱导痰中细胞学分类,记录咳嗽症状评分。结果: 支气管舒张剂治疗无效组患者诱导痰中IL-8水平明显高于支气管舒张剂治疗有效组和正常对照组(P<0.05);诱导痰中ECP水平明显低于支气管舒张剂治疗有效组(P<0.05),但与正常组水平接近(P>0.05);支气管舒张剂治疗无效组患者诱导痰中中性粒细胞百分比明显高于支气管舒张剂治疗有效组和正常对照组(P<0.05);支气管舒张剂治疗无效组患者咳嗽症状评分与诱导痰中IL-8水平呈正相关关系(r=0.764,P<0.01),并与其中性粒细胞百分比呈正相关关系(r=0.889,P<0.01)。结论: IL-8和中性粒细胞参与支气管舒张剂治疗无效的CVA的发病,可加重气道炎症和气道高反应性,使咳嗽症状加重;在临床上IL-8和中性粒细胞检测可作为CVA诊断、严重程度及疗效判定的辅助手段。

关键词: 白细胞介素8, 中性粒细胞, 咳嗽变异性哮喘, 支气管舒张剂

Abstract:

Objective: To determine the inflammatory factor levels and inflammatory cell percentages in the patients with cough variant asthma (CVA), and to clarify their potential role in the pathogenesis of CVA unresponsive to bronchodilator treatment.Methods: 60 patients with CVA were randomly selected and divided into CVA unresponsive to bronchodilator treatment group (n=30) and CVA responsive to bronchodilator treatment group (n=30).As the same time 30 cases of healthy persons were used as normal control group.The levels of interluekin-8(IL-8) and esoinophil cationic protein (ECP) in their induced sputum were detected, the classification of inflammatory cells in their induced sputum were observed, and their scores of cough symptom were recorded.Results: The IL-8 level in the induced sputum of the patients in CVA unresponsive to bronchodilator treatment group was higher than that in CVA responsive to bronchodilator treatment group and normal control group (P<0.05).The ECP level in the induced sputum of the patients in CVA unresponsive to bronchodilator treatment group was lower than that in CVA responsive to bronchodilator treatment group (P<0.05), but it was similar to the level in normal control group (P>0.05).The neutrophil percentages in the induced sputum of the patients in CVA unresponsive to bronchodilator treatment group were higher than those in CVA responsive to bronchodilator treatment group and normal control group (P<0.05).The scores of cough symptom of the patients in CVA unresponsive to bronchodilator treatment group was positively correlated with IL-8 level(r=0.764, P<0.01), and the scores of cough symptom of the patients in CVA unresponsive to bronchodilator treatment group was positively correlated with the neutrophil percentage in induced sputum(r=0.889, P<0.01).Conclusion: IL-8 and neutrophil may be associated with the incidence of CVA unresponsive to bronchodilator treatment.They can aggravate the inflammation and hypersensitivity of airway and cough symptom.The determination of IL-8 and neutrophil can be used as an accessory method in the diagnosis and judgement of severity degree and curative effect of CVA in clinic.

Key words: interleukin-8, neutrophil, cough variant asthma, bronchodilator

中图分类号:

R562.25

胡波, 滕飞, 王红艳, 张璐, 袁红艳. 支气管舒张剂治疗无效的咳嗽变异性哮喘患者炎性介质水平和炎性细胞百分比的检测及其意义[J]. 吉林大学学报(医学版), 2016, 42(02): 321-325.

HU Bo, TENG Fei, WANG Hongyan, ZHANG Lu, YUAN Hongyan. Determination of inflammatory factorlevels and inflammatory cell percentages in cough variant asthma patients unresponsive to bronchodilator treatment and their significances[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 321-325.

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