The Ti-6A1-4V（TC4） implants were made by 3D printed method，and the classic Straumann implants were used as controls.The animal models were established by implanting bilateral femur implants in 6 rabbits； one TC4 implant and one Straumann implant were implanted on each side，and there were 12 T4C implants（（TC4 implant group） and 12 Straumann implants （Straumann implant group） in all. At 2， 4 and 8 weeks after the operation， two rabbits in each group were killed respectively， and the tissue around the implants was taken out； toluidine blue staining was used to observe osteoblasts， new bone formation and bone tissue repair， and methylene blue acid fuchsin staining was used to observe the new bone repair and mineralization.The surface structure changes of TC4 implants after operation were observed by scanning electron microscope（SEM），and the element composition of TC4 implants after operation was analyzed by energy dispersive X-ray spectrometer（EDS）.

The toluidine blue staining results showed that at 2 weeks after the operation， the deep staining of nucleus and linear arrangement of light blue osteoblasts on the edge of bone tissue close to the implants of the rabbits in two groups were found； at 4 weeks after the operation， the existence of light stained new collagen fiber-like structure around the implants in two groups was seen， and the arrangement was not orderly； at 8 weeks after the operation，the light blue new bone formation with continuous structures was found in one side close to the implants of the rabbits in two groups， containing a large number of bone cells， and the boundary between new bone and original mineralized bone was clear. The methylene blue acid fuchsin staining results showed that at 2 weeks after the operation， the implants in two groups showed active osteoblast aggregation around the implants； at 4 weeks after the operation， new and red stained osteoid structures were found between the implants and the original mineralized bone in two groups； at 8 weeks after the operation， new bone formation with certain degree of calcification was seen on the surface of the implants in two groups， which was dark red，and the degree of calcification in Straumann group was slightly better than that in TC4 group. The SEM results showed that the TC4 implants completed in vivo experiment were basically consistent with the preoperative TC4 implants in surface structure， and no obvious structural defects were found.The results of EDS showed that the TC4 implant completed in vivo experiment contained not only the elements consistent with the preoperative TC4 implants， but also the major elements Ca， Mg and trace elements Si.

The 3D printed TC4 implants have good structural stability and biocompatibility， and can achieve the similar bone binding with Straumann implants invivo.