吉林大学学报(医学版) ›› 2021, Vol. 47 ›› Issue (3): 559-565.doi: 10.13481/j.1671-587X.20210304

• 基础研究 • 上一篇    下一篇

PRDX6过表达对肝癌细胞增殖、侵袭和迁移的影响及其分子机制

母润红1,刘馨竹2,林睿2,李雨澎1,王璐瑶2,王春雨2,郭笑2()   

  1. 1.北华大学基础医学院免疫学教研室,吉林 吉林 132013
    2.北华大学药学院药学实验室,吉林 吉林 132013
  • 收稿日期:2020-12-20 出版日期:2021-05-28 发布日期:2021-05-28
  • 通讯作者: 郭笑 E-mail:395798544@qq.com
  • 作者简介:母润红(1975-),女,吉林省吉林市人,副教授,医学博士,主要从事肿瘤免疫学方面的研究。
  • 基金资助:
    国家自然科学基金青年基金项目(3900918);吉林省科技厅科学计划项目(20190303047SF);吉林省卫健委卫生与健康创新项目(2020J024)

Effect of PRDX6 over-expression of proliferation, invasion and migration of liver cancer cells and its molecular mechanism

Runhong MU1,Xinzhu LIU2,Rui LIN2,Yupeng LI1,Luyao WANG2,Chunyu WANG2,Xiao GUO2()   

  1. 1.Department of Immunology,School of Basic Medical Sciences,Beihua University,Jilin 132013,China
    2.Department of Pharmaceutical Laboratory,School of Pharmacy,Beihua University,Jilin 132013,China
  • Received:2020-12-20 Online:2021-05-28 Published:2021-05-28
  • Contact: Xiao GUO E-mail:395798544@qq.com

摘要: 目的

探讨过表达过氧化物氧化还原蛋白6(PRDX6)基因对肝细胞癌增殖、侵袭和迁移的影响,并阐明其可能的作用机制。

方法

人肝癌HepG2和Hep3B细胞分为过表达PRDX6组(转染过表达质粒PIRES-EGFP-PRDX6)和空载体组(转染对照质粒PIRES)。实时荧光定量PCR(RT-qPCR)和Western blotting法检测2组肝癌细胞中PRDX6 mRNA和蛋白表达水平,MTT法检测2组肝癌细胞增殖活性,划痕实验检测2组肝癌细胞划痕愈合率,Transwell小室实验检测2组肝癌细胞的侵袭细胞数。Western blotting法检测2组细胞中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)蛋白表达水平。

结果

RT-qPCR和Western blotting法检测,过表达PRDX6组肝癌细胞中PRDX6 mRNA和蛋白表达水平明显高于空载体组(P<0.05)。MTT法检测,过表达PRDX6组肝癌细胞的增殖活性明显高于空载体组(P<0.05)。划痕实验和Tanswell小室实验检测,与空载体组比较,过表达PRDX6组肝癌细胞划痕愈合率和侵袭细胞数升高(P<0.05)。Western blotting检测,与空载体组比较,过表达PRDX6组肝癌细胞中MMP-2和MMP-9蛋白表达水平升高(P<0.05)。

结论

PRDX6过表达可增强肝癌HepG2和Hep3B细胞体外增殖、侵袭及迁移能力,其作用机制可能与PRDX6过表达调控MMP- 2和MMP-9蛋白表达水平有关。

关键词: 肝肿瘤, 过氧化物氧化还原蛋白6, 细胞增殖, 细胞侵袭, 细胞迁移

Abstract: Objective

To discuss the effect of over-expression of peroxiredoxin 6 (PRDX6) gene on the proliferation, invasion and migration of liver cancer cells, and to clarify its possible mechanism.

Methods

The HepG2 and Hep3B cells were established and divided into PRDX6 over-expression group(tansfected with over-expression vector PIRES-EGFP-PRDX6) and empty vector group(tansfected with control vector PIRES).The expression levels of PRDX6 mRNA and protein in the liver cancer cells in two groups were detected by Real-time fluorescence quantitative(RT-qPCR) and Western blotting methods;the proliferation rates of the liver cancer cells in two groups were detected by MTT method in vitro;scratch assay and Transwell chamber assay were used to evaluate the wound healing rate and the invasion number of liver cancer cells in two groups; Western blotting method was used to detect the expression levels of PRDX6, matrix matalloproteinases-2(MMP-2)and matrix matalloproteinases-9(MMP-9)proteins in the liver cancer cells in two groups.

Results

The RT-qPCR and Western blotting results showed that compared with empty vector group, the expression levels of PRDX6 mRNA and protein in PRDX6 over-expression group were significantly increased (P<0.05).The MTT results showed that compared with empty vector group, the proliferation activities of the liver cancer cells in PRDX6 over-expression group were significantly increased (P<0.05). The scratch assay and Transwell chamber assay results showed that compared with empty vector group, the wound healing rates and the number of invasion liver cancer cells in PRDX6 over-expression group were significantly increased (P<0.05).The Western blotting results showed that that compared with empty vector group, the expression levels of MMP-2 and MMP-9 proteins in the liver cancer cells in PRDX6 over-expression groups were increased(P<0.05).

Conclusion

PRDX6 could significantly promote the proliferation, invasion, and migration of liver cancer cells, and its mechanism may be related to its regulatory effect on the expression levels of MMP- 2 and MMP- 9 proteins.

Key words: liver neoplasms, peroxiredoxin 6, cell proliferation, cell invasion, cell migration

中图分类号: 

  • R735.7