关键词: 黑色素瘤, 实验性, 集落形成单位测定, 细胞周期

Abstract: Objective To investigate the inhibitory effects of scorpion venom (SV) and its separated components (SV-Ⅰ、SV-Ⅱ and SV-Ⅲ) on the growth of B16 melanoma cells. Methods MTT method was used to detect the survival of tumor cells treated with SV and its components in vitro and colony forming assay was applied to measure the proliferation of tumor cells. Moreover, flow cytometry was further adopted to observe the changes of cell cycle of tumor cells. Results SV-Ⅰ, SV-Ⅱ and SV-Ⅲ could inhibit the growth of B16 melanoma cells significantly at the concentration of 200, 400 and 800 mg•L^-1 (P<0.05~P<0.01) and SV could inhibit the growth of B16 melanoma cells at 400 and 800 mg•L^-1 (P<0.01~P<0.05). Furthermore, SV and its components could inhibit colony forming of B16 melanoma cells (P<0.01) at 400 and 800 mg•L^-1. Cells in G₀/G₁ stage were decreased and cells in G₂+M stage were increased obviously (P<0.05~P<0.01) after　SV-Ⅰ, SV-Ⅱ and SV-Ⅲ were given at 400 and 800 mg•L^-1, respectively. Conclusion SV and its components can inhibit the proliferation and survival of B16 melanoma cells. Moreover, the inhibitory effects of components are better than those of SV on B16 melanoma cells. Changes of cell cycle will be an important mechanism of inhibitory effects of SV.

Key words: melanoma, experimental, colony-forming units assay, cell cycle