三焦针法通过激活SDF-1α/CXCR4通路对快速老化模型小鼠学习和记忆能力的改善作用

doi:10.13481/j.1671-587X.20210302

Abstract

Abstract: Objective

To investigate the improvement effect of Sanjiao acupuncture on the neurological function and stromal cell-derived factor-1α/chemokine receptor 4 （SDF-1α/CXCR4） pathway of the rapid aging model （SAMP8）mice， and to explore the mechanism of Sanjiao acupuncture in the SAMP8 mice.

Methods

The experiment was divided into model group （SAMP8 mice）， non-acupuncture group （SAMP8 mice + non-acupuncture）， acupuncture group （SAMP8 mice + acupuncture）， acupuncture + AMD3100 group （SAMP8 mice + acupuncture + AMD3100 treatment），and control group （SAMR1 mice），and there were 10 mice in each group. Morris water maze test was used to detect the learning and memory function indexes of the mice in various groups； enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of amyloid β （Aβ） 1-42 in hippocampus tissue of the mice in various groups； choline acetyltransferase （chAT） and acetylcholinesterase （AchE） kits were used to detect the activities of chAT and AchE in hippocampus tissue of the mice in various groups； Western blotting method was used to detect the expression levels of SDF-1α and CXCR4 proteins in hippocampus tissue of the mice in various groups.

Results

Compared with control group， the escape latency of the mice in model group was prolonged（P<0.05）， the level of Aβ1-42 in hippocampus tissue of the mice was increased （P<0.05）， the number of crossing the platform， the ratio of swimming distance in the original quadrant to total distance， the ratio of swimming time in the original quadrant to total time， the activities of chAT and AchE in hippocampus tissue，and the levels of SDF-1α and CXCR4 proteins in hippocampus tissue of the mice were decreased （P<0.05）； compared with model group， the escape latency of the mice in acupuncture group was shortened（P<0.05）， the level of Aβ1-42 in hippocampus tissue of the mice was decreased （P<0.05）， the number of crossing the platform， the ratio of swimming distance in the original quadrant to total distance， and the ratio of swimming time in the original quadrant to total time， the activity of chAT in hippocampus tissue，and the expression levels of SDF-1α and CXCR4 proteins in hippocampus tissue of the mice were increased （P<0.05）； compared with acupuncture group， the escape latency of the mice in acupuncture + AMD3100 group was prolonged（P<0.05）， the level of Aβ1-42 in hippocampus tissue of the mice was increased （P<0.05），the number of crossing the platform， the ratio of swimming distance in the original quadrant to total distance， and the ratio of swimming time in the original quadrant to total time， the activity of chAT in hippocampus tissue，and the expression levels of SDF-1α and CXCR4 proteins in hippocampus tissue of the mice were decreased（P<0.05）.

Conclusion

Sanjiao acupuncture may reduce the accumulation of Aβ1-42 by activating the SDF-1α/CXCR4 pathway， and improve the neurological function of the SAMP8 mice.

Key words: stromal cell derived factor-1α/chemokine receptor 4 pathway, Sanjiao acupuncture, rapid aging model, neurological function

CLC Number:

R245.3

Yu WANG, Lan ZHAO, Bohong KAN, Huiyan SHI. Improvement effect of Sanjiao acupuncture on learning and memory abilities of rapid aging model mice by activating SDF-1α/CXCR4 pathway[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 545-550.

Figures/Tables 5

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Tab. 3

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Tab. 4

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Group	Escape latency(t/s)	Number of crossing platform	Original quadrant swimming distance/total distance	Original quadrant swimming time/total time
Control	23.16±3.18	7.49±1.38	0.41±0.05	0.43±0.04
Model	57.15±4.18^*	2.38±0.56^*	0.21±0.02^*	0.21±0.02^*
Non-acupoint acupuncture	54.38±5.44^*	2.38±0.21^*	0.21±0.01^*	0.20±0.02^*
Acupoint acupuncture	38.49±4.12^*△#	5.55±0.49^*△#	0.38±0.04^*△#	0.35±0.04^*△#
Acupoint acupuncture+AMD3100	47.98±4.08^*△#○	3.75±0.42^*△#○	0.28±0.02^*△#○	0.26±0.02^*△#○
F	104.751	90.595	87.700	109.659
P	0.000	0.000	0.000	0.000

Group	Aβ1-42
Control	1.86±0.26
Model	7.65±0.57^*
Non-acupoint acupuncture	7.54±0.61^*
Acupoint acupuncture	3.86±0.32^*△#
Acupoint acupuncture+AMD3100	5.28±0.44^*△#○
F	287.909
P	0.000

Group	chAT[λ_B/(IU·g^－1)]	AchE[λ_B/(U·mg^－1）]
Control	163.45±15.89	0.56±0.08
Model	86.34±6.48^*	0.48±0.04^*
Non-acupoint acupuncture	89.64±9.12^*	0.49±0.05
Acupoint acupuncture	134.19±13.48^*△#	0.52±0.04
Acupoint acupuncture+ AMD3100	98.18±8.43^*○	0.50±0.06
F	88.452	3.121
P	0.000	0.024

Group	SDF-1α	CXCR4
Control	0.89±0.06	0.56±0.04
Model	0.18±0.03^*	0.11±0.02^*
Non-acupoint acupuncture	0.16±0.02^*	0.10±0.01^*
Acupoint acupuncture	0.59±0.05^*△#	0.59±0.04^△#
Acupoint acupuncture+ AMD3100	0.37±0.03^*△#○	0.39±0.04^*△#○
F	566.084	526.887
P	0.000	0.000

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Improvement effect of Sanjiao acupuncture on learning and memory abilities of rapid aging model mice by activating SDF-1α/CXCR4 pathway

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