基于HMMR在肺腺癌组织中表达水平及其对LUAD患者预后影响的生物信息学分析

doi:10.13481/j.1671-587X.20210621

Abstract

Abstract: Objective

To investigate the expression of hyaluronan-mediated motility receptor （HMMR） in the lung adenocarcinoma （LUAD） tissue and its impact on the prognosis of the LUAD patients， and to elucidate the role of HMMR in the occurrence and development of LUAD.

Methods

The expression levels HMMR mRNA and protein in the LUAD tissue were assessed by Gene Expression Omnibus （GEO）， the Cancer Genome Atlas （TCGA） and Human Protein Atlas （HPA）. The effect of HMMR expression on the overall survival （OS） and disease-free survival （DFS） of the LUAD patients was analyzed by using the GEPIA2 database. The relationships between HMMR mRNA expression and the clinicopathological parameters and prognosis of the LUAD patients were analyzed by using the TCGA database. The GSEA enrichment analysis was used to explore the pathways significantly enriched for HMMR in the LUAD tissue. The relationship between HMMR mRNA expression and TP53 gene mutations was probed by using the UALCAN database， and the interaction between HMMR and the proteins in the p53 signaling pathway was predicted by the Search Tool for Recurring Instances of Neighbouring Genes （STRING） database.

Results

The expression levels of HMMR mRNA and protein in LUAD tissue were significantly higher than those in normal and adjacent tissues （P<0.01）. The patients with higher HMMR mRNA expression had shorter OS and DFS（P<0.01）. In LUAD tissue， the HMMR mRNA expression was associated with the patients’ TNM stage，T stage， and N stage （P<0.01）.The univariate and multivariate Cox analysis results showed that the OS of the patients with high HMMR mRNA expression was shorter. The GSEA enrichment analysis results showed that cell cycle， homologous recombination， p53 signaling pathway， bladder cancer， pancreatic cancer， small cell lung cancer， and ubiquitin-mediated proteolysis pathways were significantly enriched in the high expression phenotype of HMMR.

Conclusion

The expression of HMMR in LUAD tissue is significantly up-regulated，and the highly expressed HMMR mRNA is an independent evaluation indicator of poor prognosis in the LUAD patients.

Key words: lung adenocarcinoma, hyaluronan-mediated motility receptor, database, prognosis

CLC Number:

R734.2

Shuzhen LI,Yajie CAO,Haiying GENG,Zengxiaorui CAI,Chunmei DAI,Youfeng WEN,Ning LI. Bioinformatics analysis based on expression level of HMMR in lung adenocarcinoma tissue and its impact on prognosis of LUAD patients[J].Journal of Jilin University(Medicine Edition), 2021, 47(6): 1502-1509.

Figures/Tables 9

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References 29

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Bioinformatics analysis based on expression level of HMMR in lung adenocarcinoma tissue and its impact on prognosis of LUAD patients

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