To study the effect of integrin-associated protein （IAP）CD47 knockout on the angiogenesis after traumatic brain injury （TBI） in the mice， and to clarify its possible molecular mechanism.

In the in vivo experiment， the TBI models of adult C57BL/6 mice were prepared by hydraulic craniocerebral percussion instrument， and the experimental mice were randomly divided into sham operation group and TBI group. The brain tissue was collected 24 h after TBI for the detection of water content in brain tissue； Evans blue（EB） injection was used to detect the blood brain barrier permeability（EB level） and HE staining was used to observe the pathomorphology of brain tissue of the mice. Immunofluorescence staining method was performed to detect the expression levels of vascular endothelial growth factor receptor 1（VEGFR1） and platelet-endothelial cell adhesion molecule CD31 proteins in brain tissue of the mice. In the in vitro experiment， the C57BL16 mice with CD47 knockout and endogenous expression of green fluorescent protein（GFP） labeled （CD47KO-GFP group） and wild type （WT） mice （WT group） were selected.The TBI models were prepared with hydraulic craniocerebral percussion.The brain microvascular endothelial cells were extracted and mixedly cultured. The regeneration and tube-forming capacities of cerebral microvascular endothelial cells after TBI were observed by fluorescence microscope； immunofluorescence staining method was used to detect the activation of nuclear factor-κB（NF-κB） in the cerebral microvascular endothelial cells in injured area of the mice.

Compared with sham operation group， the water content in brain tissue and EB level of the mice in TBI group were significantly increased （P<0.05）. The HE staining results showed that compared with sham operation group， the brain tissue structure of the mice in TBI group was unrecognizable and the bleeding spots were obvious. The immunofluorescence staining results showed that compared with sham operation group， the expression levels of VEGFR1 and CD31 proteins in brain tissue of the mice in TBI group were significantly increased（P<0.01）. The fluorescence microscope results showed that compared with WT group，the number of brain microvascular endothelial cells participating in angiogensis of the mice in CD47KO-GFP group was significantly increased（P<0.05）. The immunofluorescence staining results showed that compared with WT group，the activation of NF-κB in the cerebral microvascular endothelial cells in CD47KO-GFP group was decreased significantly.

After TBI， the blood brain barrier permeability and angiogenesis of injured brain tissue are increased significantly， and CD47 knockout can promote the angiogenesis.

To use the mouse macrophage cell line RAW264.7 as the research object and explore the effect of high glucose microenvironment on the polarization of mouse macrophages in vitro，and to clarify the regulatory effect of miR-125b.

The RAW264.7 cells were divided into normal control （NG）group，normal glucose inhibition （NG+ miR-125b inhibitor） group，high glucose stimulation （HG） group and high glucose inhibition （HG+ miR-125b inhibitor） group. The cells in each group were cultured in vitro for 72 h， and the cells and supernatant were collected. RT-qPCR method was used to detect the expression levels of M1/M2 polarization-related genes and the expression levels of miR-125b in the cells in various groups.ELISA method was used to detect the levels of M1/M2 polarization-related cytokines in supernatant of the cells in various groups； flow cytometry was used to detect the positive expression rates of M1/M2 polarization surface markers CD86 （M1 marked） and CD206（M2 marked）.The expression of miR-125b in the cells was silenced through miR-125b inhibitor， and RT-qPCR and Western blotting methods were used to detect the expression levels of key transcription factors interferon regulator 4 （IRF4），interferon regulator 5 （IRF5）， and peroxisome-activated receptor （PPAR） mRNA and proteins associated with M1/M2 polarization in the cells in various groups.

After treated with high glucose for 72 h， compared with NG group， the IL-10 mRNA expression level and the level of IL-10 in the cell supernatant in HG group were significantly decreased （P<0.01）， while the expression levels of IL-1β and TLR4 mRNA were significantly increased（P<0.01）， and the levels of IL-1β，IL-6， and IL-12 in the cell supernatant were increased （P<0.01）.Compared with NG group， the positive expression rate of CD86 in HG group was significantly increased（P<0.05）， while the positive expression rate of CD206 was significantly decreased （P<0.05）； at the same time， the expression level of miR-125b was significantly increased （P<0.01）. The expression level of miR-125b was silenced by miR-125b inhibitor， compared with NG and HG groups respectively， the expression levels of miR-125b after transfection in NG+miR-125b inhibitor group and HG+miR-125b inhibitor were decreased significantly （P<0.01）， the IL-10 mRNA expression levels in the cells and the levels of IL-10 in the cell supernatant were significantly increased （P<0.01）， while the expression levels of IL-1β and TLR4 mRNA and the levels of IL-12， IL-6， and IL-1β in the cell supernatant were decreased （P<0.05 or P<0.01）； the positive expression rates of CD86 in the cells were significantly decreased（P<0.05 or P<0.01）， and the positive expression rates of CD206 were significantly increased（P<0.05）.Compared with NG group， the expression levels of IRF4， PPAR mRNA and proteins in HG group were significantly decreased （P<0.01）， while the expression levels of IRF5 mRNA and protein were increased （P<0.01）； after silencing the expression of miR-125b， compared with NG group， the mRNA and protein expression levels of IRF4 and PPAR in the cells in NG+miR-125b inhibitor group were increased significantly（P<0.01）， while the expression levels of IRF5 mRNA and protein were decreased（P<0.05）.Compared with HG group，the expression levels of IR4 and PPAR mRNA and proteins in the cells in HG+miR-125b inhibitor group were decreased（P<0.01），while the expression level of IRF5 miRNA was significantly decreased（P<0.01）.

High glucose stimulation can induce to the macrophage polarization to the pro-inflammatory M1 phenotype， and the expression level of miR-125b is also significantly increased. Silencing the expression of miR-125b can partially reverse the macrophages induced by high glucose stimulation to the pro-inflammatory type change，and this process may be achieved by up-regulating IRF4， PPAR and down-regulating IRF5.

To investigate the effects of limonin on the lipid metabolism and intestinal microbiota in the rats with nutritional obesity induced by high fat diet， and to clarify its possible mechanism.

The young rats （3 weeks） were randomly divided into control group， model group， low dose of limonin group （12.5 mg·kg^－1）， medium dose of limonin group （25 mg·kg^－1） and high dose of limonin group （50 mg·kg^－1）， with 10 rats in each group. Except for control group， the rats in other groups were fed with high-fat diet to establish the nutritional obesity rat models. After successful modeling， limonin was administered in the rats in low，medium and high doses of limonin groups，once a day for 4 weeks. The body weights of rats in various groups were recorded before and after administration， and the Lee’s index and fat coefficient were calculated. The levels of total cholesterol （TC）， triglyceride （TG）， low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） in serum of the rats in various groups were detected. HE staining was used to observe the pathomorphology of liver tissue and the fatty deposition in liver tissue of the rats in various groups.The structural changes of intestinal microbiota in the rat feces were analyzed by 16S rRNA sequencing.

Compared with control group， the body weight， Lee’s index and adipose coefficient of the rats in model group were significantly increased （P<0.05）， the levels of TC， TG and LDL-C in serum were significantly increased （P<0.05）， and the HDL-C level was significantly decreased （P<0.05）； steatosis and adipose accumulation in liver could be seen； in the feces， the relative abundance of Firmicutes and Spirillae was significantly increased （P<0.05）， while the relative abundance of Bacteroidetes and Lactobacillus was significantly decreased （P<0.05）. Compared with model group， the body weights， Lee’s indexes and adipose coefficients in medium and high doses of limonin groups were significantly decreased（P<0.05）， the levels of TC，TG and LDL-C in serum were significantly decreased （P<0.05）， and the HDL-C levels were significantly increased（P<0.05）； steatosis and adipose accumulation in liver were significantly improved； in the feces， the relative abundance of Firmicutes and Spirillae was significantly decreased （P<0.05），while the relative abundance of Bacteroidetes and Lactobacillus was significantly increased （P<0.05）.There were no significant differences in the above indexes between low dose of limonin group and model group （P>0.05）.

Limonin can improve obesity in the nutritional obesity rats by regulating lipid metabolism and gut microbiota structure.

To study the expression patterns of integrin β1 and laminin receptor 1 （LAMR1） in the mouse molar germs at different stages of molar development， and to explore the possible mechanism and significance of integrin β1 and LAMR1 in mouse molar germ development.

The mice on the embry on days 13.5 （E13.5）， E14.5， E16.5， E18.5 and postnatal days 5 （PN5） were obtained， and used as E13.5，E14.5，E16.5E18.5，and PN5 groups，with 5 mice in each group；their heads were separated， immobilized， decalcified， dehydrated， embedded and sliced， and the morphology of tooth embryo tissue at different stages was observed by HE staining. The expression levels of integrin β1 and LAMR1 in the molar germ tissue at different stages of mouse molar development were observed by immunohistochemical staining.

The HE staining results showed that the tooth germ tissue development of the mice in E13.5 group corresponded to the bud stage， the epithelial cells protruded into the ectodermal mesenchyma and formed epithelial buds like flower buds；the tooth germ tissue development of the mice in E14.5 group corresponded to the cap stage， the epithelial bud volume was increased， which was called the enamel organ at the cap stage， and the density of ectomesenchymal cells around the enamel organ was increased， forming the dental papilla； the tooth germ tissue development of the mice in E16.5 group corresponded to the bell shaped early stage， the enamel organ further grew， resembled a hanging bell， and initially had the shape of the cusp； the tooth germ tissue development of the mice in E18.5 group corresponded to the late bell shaped stage， the ameloblast further developed， the inner ameloblast differentiated forward， and the shape changed from cubic to high columnar； the tooth crown of the mice in PN5 group developed into cervical loop， epithelial root sheath， epithelial septum and other structures， the ameloblasts and odontoblasts differentiated and matured and arranged neatly in high columnar shape， and enamel and dentin were formed. The results of immunohistochemical staining showed that integrin β1 and LAMR1 were expressed in the epithelium and dental papilla of mouse molars at different stages of molar development， and the expression intensities of integrin β1 and LAMR1 in different parts gradually were increased with the process of cell differentiation. The expression levels of integrin β1 protein in the ameloblasts of the mice in PN5 group was higher than those in the inner ameloblasts / preameloblasts in germ tissue of the mice in E14.5， E16.5 and E18.5 groups（P<0.05）； the expression levels of integrin β1 protein in the odontoblasts in tooth germ tissue of the mice in PN5 group were higher than those in the preodontoblasts in dental germ of the mice in E14.5， E16.5 and E18.5 groups（P<0.05）. The expression levels of LAMR1 protein in the inner ameloblasts / preameloblasts in E16.5 and E18.5 groups were higher than that in the inner ameloblasts in E14.5 group（P<0.05）， and were lower than that in the ameloblasts in dental germ of the mice in PN5 group（P<0.05）； the expression level of LAMR1 protein in the preodontoblasts in E18.5 group was higher than that in the preodontoblasts in E16.5 group（P<0.05）， and was lower than that in the odontoblasts in dental germ of the mice in PN5 group（P<0.05）. In PN5 group， the expression levels of integrin β1 and LAMR1 proteins in the mature odontoblasts at the cusp were higher than those in the immature odontoblasts between the apical and the neck （P<0.05）.

Integrin β1 and LAMR1 are expressed in the basement membranes， （pre）ameloblasts and （pre）odontoblasts during molars development in the mice. They may be involved in signal transduction of extracellular matrix and play a promoting role in the differentiation and polarity formation of the ameloblasts and odontoblasts.

To explore the effects of altitude anoxic environment on the hearing of rats that migrated from the plain to the plateau，and to elucidate the molecular mechanism of sound acclimation in the rats.

A total of 72 Wistar rats were divided into plain control group，migration plateau 2 months group，migration plateau 4 months group and migration plateau 10 months group，with 18 rats in each group. The auditory brainstem response evoked by click（ABR）thresholds and latencies of the rats were detected at 2， 4 and 10 months after migration to the plateau. The ultrastructures of neurons and myelin sheath in cochlear tissue of the rats were observed by transmission electron microscope. The expression levels of growth associated protein-43（GAP-43）and myelin basic protein （MBP） mRNA and proteins in cochlear tissue of the rats in various groups were analyzed by real-time fluorescence quantitative PCR （RT-qPCR） method，Western blotting method and immunohistochemistry method.

Compared with control group， the thresholds of ABR of the rats in migration plateau groups were increased for 5－13 dB， but there were no statistical differences（P>0.05）. The lantencies of ABR of rats were detected after stimulated with Crik of 80 dB SPL；compared with control group，the lantencies of wave Ⅰ and Ⅲ of the rats in migration plateau 2 months group and migration plateau 4 months group were prolonged（P<0.05）.The transmission electron microscope results showed that myelin sheath structure appeared intact and lamellar arrangement was close and orderly in control group； the most serious myelin injury occurred at the time of 2 months after the migration to the plateau， appeared the lamellar arrangement separated， the myelin sheaths around neurons were broken， and the space between cells and myelin sheaths was increased； meanwhile， the abnormal mitochondrial structure was also found. At the time of 4 months after the migration to the plateau， the myelin lesion was relieved， and at the time of 10 months， the myelin structure was almost normal. The results of RT-qPCR and Western blotting methods showed that compared with control group， the expression levels of MBP mRNA and protein in cochlear tissue of the rats in migration plateau 2 months group and migration 4 months group were decreased（P<0.01）；but the expression levels of MBP and GAP-43 mRNA and protein in cochlear tissue of the rats in migration plateau 10 months group had no significant differences（P>0.05）.Compared with control group，the expression levels of GAP-43 mRNA and protein in cochlear tissue of the rats in migration plateau 2 months group were decreased（P<0.01）. Compared with plateau migration 2 months group， the expression levels of GAP-43 mRNA and protein in cochlear tissue of the rats in migration plateau 4 months group were increased（P<0.05）.

High altitude hypoxic environment can cause the peripheral auditory nerve myelin injury， lead to the prolonged ABR latency and affect the auditory signal transduction. Long-term hypoxic environment can induce myelin sheath regeneration and induce auditory adaptive repair.

To investigate the biocompatibility of rabbit acellular cartilage matrix particles （ACM）， and to clarify its promotion effect on the endochondral osteogenesis of adipose tissue-derived stem cells （ADSCs） in the SD rats.

The primary ADSCs of SD rabbits were extracted for three-dimensional differentiation and flow cytometry was used to detect the cell dryness.The ear cartilage of 3-month-old New Zealand white rabbits was collected and treated with grinding and soaking method to prepare ACM. The experiment was divided into ACM and ACM+ADSCs co-culture group.The ultrastructures of ACM and the ADSCs in ACM were observed under scanning electron microscope，and the growth status of ADSCs in ADSCs+ACM co-culture group was by Calcein-AM/PI fluorescence staining method. The ADSCs were divided into control group and experimental group（ADSCs and ACM co-culture）.The CCK-8 method was used to detect the proliferation activities of cells in two groups. Real-time fluorescence quantitative PCR （RT-qPCR） was used to detect expression levels of type Ⅱ collagen （COL-Ⅱ），type Ⅹ collagen （COL-Ⅹ）， SRY-related high mobility group-box 9 （SOX-9），vascular endothelial growth factor （VEGF）， alkaline phosphatase （ALP） and Runt-related transcription factor 2 （RUNX-2） mRNA in the ADSCs in two groups at 7 and 14 d of chondrogenic induction.

The rabbit ACM was successfully prepared. The extracted ADSCs could differentiate in three ways and the specific surface markers of stem cells were expressed in the ADSCs. The ADSCs could adhere to ACM. Compared with control group， the proliferation activity of the cells in experimental group was increased （P<0.05）.The RT-qPCR results showed that compared with control group， the expression levels of VEGF，ALP and RUNX-2 mRNA in the ADSCs in experimental group were increased at 7 d of chondrogentic induction （P<0.05）， and the expression levels of COL-2， SOX-9， and COL-Ⅱ mRNA had no significant differences（P>0.05）. At 14 d of chondrogentic induction， compared with control group， the expression levels of VEGF， ALP， and RUNX-2 mRNA in the ADSCs in experimental group were increased （P<0.05），and the expression level of COL-Ⅹ mRNA was decreased （P<0.05）. Compared with 7 d of chodrogenic induction， the expression levels of VEGF， ALP， and RUNX-2 mRNA in the ADSCs in control group at 14 d of chodrogenic induction were decreased （P<0.05）， and the expression levels of COL-Ⅱ and COL-Ⅹ mRNA were increased （P<0.05）. Compared with 7 d of chondrogenic induction， the expression levels of COL-Ⅱ and COL-Ⅹ mRNA in the ADSCs in experimental group were increased at 14 d of chondrogenic induction （P<0.05）， while the expression levels of VEGF， ALP， and RUNX-2 mRNA were decreased（P<0.05）.

The rabbit ACM lack immunogenicity and have good biocompatibility and chondrogenic induction ability， which is beneficial to the occurrence of endochondral osteogenesis.

To study the effects of genistein chromium complex genistein chromium on the glucose metabolism and lipid metabolism in the obese rats induced bymonosodium L- glutamate （MSG）， and to provide the evidence for developing new drugs for obese type 2 diabetes.

MSG was used to estblish the obese rat models. Fifty female modeling rats （whose body mass exceeded 20% of the average body mass of the rats in control group） were randomly divided into model group， metformin group （95 mg·kg^－1）， low dose （15 mg·kg^－1）， medium dose （30 mg·kg^－1） and high dose （60 mg·kg^－1） of genistein chromium groups， another 10 normal rats were used as control group； the test drug was administered by gavage once a day for 4 weeks. After administration， the vein blood was obtained，and the levels of fasting blood glucose （Glu），triglyceride （TG） and total cholesterol （TC） in serum of the rats were measured by oxidase method；the levels of serum glycosylated hemoglobin （GHb） and insulin （INS）， nitric oxide synthase （NOS）， angiotensin Ⅱ （AngⅡ）， endothelin-1（ET-1）， prostacyclin（PGI2）， tumor necrosis factor-α （TNF-α），interleukin-6 （IL-6） were measured by enzyme-linked immunosorbent assay.

Compared with control group， the body masses of rats in the other groups were increased significantly （P<0.05） before and after administration， which proved that metformin group and the models were constructed. After 4 weeks， compared with model group， the body masses of the rats in metformin group and medium and high doses of genistein chromium groups were decreased significantly （P<0.05）；the levels of Glu， GHB and INS in serum of the rats in metformin group and different doses of genistein chromium groups were decreased significantly （P<0.05）； the insulin sensitivity index （ISI） of the rats in metformin group and medium and high doses of genistein chromium groups were increased significantly （P<0.05）； the levels of TG， TC， NOS， AngⅡ， ET-1，TNF-α，IL-6 in serum of the rats in medium and high doses of genistein chromium groups were decreased significantly （P<0.05），and the serum PGI2 levels were increased（P<0.05）； the levels of TC， AngⅡ，TNF-α，IL-6 in serum of the rats in metformin group were decreased significantly （P<0.05）.

Genistein chromium can significantly improve glucose metabolism and lipid metabolism in the MSG-induced obese rats. This effect is related to increasing the insulin sensitivity， improving the endothelial factor and inhibiting the inflammatory factor function.

To investigate the effects of platelet-derived growth factor D （PDGF-D） on the proliferation， migration and invasion of lung cancer H1299 cells， and to elucidate their possible mechanisms.

The lung cancer H1299 cells were divided into control group （transfected with empty plasmid） and PDGF-D group （transfected with GV230-PDGF-D plasmid）； meanwhile，blank group was set up.Real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods were used to detect the expression levels of PDGF-D mRNA and the expression amounts of PDGF-D protein in the cells in various groups.The H1299 cells were divided into control group（transfected with empty vector），PDGF-D group（transfected with GV230-PDGF-D plasmid），PDGF-D+PD98059（transfected with GV230-PDGF-D plasmid and treated with PD98059，an inhibitor of ERK）， and PD98059 group；the final concentration of PD98059 was 10 μmol·L^－1. MTT assay was used to detect the proliferation activities of cells in various groups. The migration rates of cells in various groups were detected by scratch test.The number of invasion cells in various groups was detected by Transwell assay. The expression levels of phosphorylated signal extracellular regulatory enzyme （p-ERK）， extracellular signal regulatory enzyme （ERK）， zinc finger transcription factor Snail，matrix metalloproteinases-1 （MMP-1） and transmembrane adhesion glycoproteins CD44 proteins in various groups were detected by Western blotting method.

The results of RT-qPCR method showed that compared with blank group and control group， the expression levels of PDGF-D mRNA in the cells in PDGF-D group were significantly increased （P<0.01）. The Western blotting results showed that there was no PDGF-D protein expression in the cells in blank group and control group；compared with blank group and control group，the expression amount of PDGF-D protein in PDGF-D group was siginificantly increased.Compared with control group， the proliferation activity of the cells and the migration rate in PDGF-D were significantly increased （P<0.05 or P<0.01）， the number of invasion cells was significantly increased （P<0.01）， and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were increased （P<0.05）.Compared with PDGF-D group， the proliferation activity of the cells and the migration rate in PDGF-D+PD98059 group were significantly decreased （P<0.05），the number of invasion cells was decreased （P<0.05），and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were decreased （P<0.05）.Compared with PDGF-D+PD98059 group， the proliferation activity of the cells and the migration rate in PD98059 group were significantly decreased （P<0.05 or P<0.01）， the number of invasion cells was decreased （P<0.01）， and the expression levels of p-ERK， Snail， MMP-1 and CD44 proteins in the cells were decreased （P<0.05 or P<0.01）.

PDGF-D can promote the proliferation， migration and invasion of lung cancer H1299 cells， and their mechanisms may be related to the up-regulation of Snail， MMP-1 and CD44 protein expressions through ERK signaling pathway.

To investigate the intervention effect of tanshinone ⅡA（Tan ⅡA） in the neonatal rats with hypoxic-ischemic encephalopathy， and to clarify its mechanism.

Forty SD rats were randomly divided into sham operation group， model group， Tan ⅡA group and miR-132 antagomir group， with 10 rats in each group. The rat models of hypoxic-ischemic encephalopathy were established by double ligation of the common carotid artery and being put into anoxic chamber. The rats in TanⅡA group were intrapeitoneally injected with Tan ⅡA injection （24 mg·kg^－1）， and 2 μg miR-132 negative control （NC） was injected into the bilateral ventricles. The rats in sham operation group and model group were intraperitoneally injected with the same amount of normal saline. After modeling， the rats in miR-132 antagomir group were intraperitoneally injected with tanshinone ⅡA injection （24 mg·kg^－1）， and 2 μg miR-132 antagomir was injected into the bilateral ventricles； the tars in various groups were injected for 7 d. The expression levels and fluorescence intensities of miR-132 in hippocampus tissue of the rats in various groups were detected by real-time fluorescence quantitative PCR（RT-qPCR） and fluorescence in situ hybridization. Modified neurological deficit score was used to evaluate the neural function of the rats in various groups. HE staining was used to observe the pathomorphology of hippocampus tissue of the rats in various groups. TUNEL assay was used to detect the apoptotic rates of neurons in hippocampus tissue of the rats in various groups. The levels of monoamine neurotransmitters in hippocampus tissue of the rats in various groups were detected by enzyme-linked immunosorbent assay （ELISA）. The densities of dendritic spines in hippocampus tissues of the rats in various groups were detected by Golgi staining. The protein expression levels of postsynaptic density-95（PSD-95），growth associated protein-43（GAP-43），microtubule-associated protein-2 （MAP）-2 and brain-derived neurotrophic factor （BDNF） in hippocampus tissue of the rats in various groups were detected by Western blotting method.

Compared with sham operation group， the expression level of miR-132 in the hippocampus tissue and the fluorescence intensity of the rats in model group were significantly decreased （P<0.05）； compared with model group， the expression level of miR-132 in hippocampus tissue and the fluorescence intensity of the rats in Tan ⅡA group were significantly increased （P<0.05）； compared with Tan ⅡA group， the miR-132 expression level in hippocampus tissue and the fluorescence intensity of the rats in miR-132 antagomir group were significantly decreased （P<0.05）. Compared with sham operation group， neurological deficit score of the rats in model group were significantly increased （P<0.05）； compared with model group， the neurological deficit score of the rats in Tan ⅡA group was significantly decreased （P<0.05）； compared with Tan ⅡA group， the neurological deficit score of the rats in miR-132 antagomir group was significantly increased （P<0.05）.The HE staining results showed that the hippocampus tissue of the rats in sham operation group was not damaged；compared with sham operation group， the hippocampus tissue of the rats in model group was seriously damaged；compared with model group， the damage of hippocampus tissue of the rats in Tan ⅡA group was improved；compared with Tan ⅡA group， the hippocampus tissue of the rats in miR-132 antagomir group was seriously damaged. Compared with sham operation group， the apoptotic rate of cells of hippocampal tissue of the rats in model group was significantly increased （P<0.05）； compared with model group， the apoptotic rate of cells of hippocampus tissue in the Tan ⅡA group was significantly decreased （P<0.05）； compared with Tan ⅡA group， the apoptotic rate of cells of hippocampus tissue in miR-132 antagomir group was significantly increased （P<0.05）. Compared with sham operation group， the levels of norepinephrine， dopamine and 5-hydroxytryptamine in hippocampus tissue of the rats in model group were significantly decreased （P<0.05）； compared with model group， the levels of norepinephrine， dopamine and 5-hydroxytryptamine in hippocampus tissue of the rats in Tan ⅡA group were significantly increased （P<0.05）； compared with Tan ⅡA group， the levels of norepinephrine， dopamine and 5-hydroxytryptamine in hippocampus tissue of the rats in miR-132 antagomir group were significantly decreased （P<0.05）. Compared with sham operation group， the density of dendritic spines in hippocampus tissue of the rats in model group was significantly decreased （P<0.05）； compared with model group， the density of dendritic spines in hippocampus tissue of the rats in Tan ⅡA group was significantly increased （P<0.05）； compared with Tan ⅡA group， the density of dendritic spines in hippocampus tissue of the rats in miR-132 antagomir group was significantly decreased （P<0.05）. Compared with sham operation group， the expression levels of PSD-95， GAP-43， MAP-2 and BDNF proteins in hippocampus tissue of the rats in model group were significantly decreased （P<0.05）； compared with model group， the expression levels of PSD-95， GAP-43， MAP-2 and BDNF proteins in hippocampus tissue of the rats in Tan ⅡA group were significantly increased （P<0.05）； compared with Tan ⅡA group， the expression levels of PSD-95， GAP-43， MAP-2 and BDNF proteins in hippocampus tissue of the rats in miR-132 antagomir group were significantly decreased （P<0.05）.

Tan ⅡA can up-regulate the expression of miR-132 in the hippocampus tissue of the neonatal rats with hypoxic-ischemic encephalopathy， and improve the neurological function and pathological damage of hippocampus tissue； its mechanism may be related to inhibiting the apoptosis of hippocampal neurons， increasing the level of neurotransmitters， the density of dendrite spines， preprotrusional proteins and the expressions of MAP-2 and BDNF proteins.

To explore the effects of histone deacetylase inhibitor （HDACi） sodium butyrate （NABT） alone or combined with X-ray irradiation on the apoptosis of human non-small cell lung cancer A549 cells， and to clarify its mechanism.

The A549 cells in logarithmic growth phase were divided into control group， NaBt group， 4 Gy X-ray irradiation group and NaBt combined with 4 Gy X-ray irradiation group. Flow cytometry was used to detect the apoptotic rates at 24 and 48 h after treatment. After 6 h of irradiation， the mitochondrial membrane potential （Δψm） and ROS （reative oxygen species） levels in the cells of various groups were analyzed by flow cytometry. After 24 h of irradiation， the expression levels of caspase-3 and p21 mRNA in the cells in various groups were detected by real-time quantitative PCR （RT-qPCR）.

After 4 Gy irriadiation for 24 and 48 h， compared with control group， 4 Gy X-ray irradiation group and NaBt group， the apoptotic rate of cells in NaBt combined with 4 Gy X-ray irradiation group was significantly increased （P<0.05 or P<0.01）. Compared with control group， the apoptotic rate of cells in NaBt group was significantly increased after 48 h of irradiation （P<0.01）. After treated with NaBt alone or combined with 4 Gy X-ray for 6 h， compared with control group， 4 Gy X-ray irradiation and NaBt group， the Δψm of A549 cells in NaBt combined with 4 Gy X-ray irradition group was decreased （P<0.05）. Compared with control group， the ROS levels in NaBt group and NaBt combined with 4 Gy X-ray irradiation group were increased （P<0.05）. Compared with 4 Gy X-ray irradiation group， the ROS level in NaBt combined with 4 Gy X-ray irradiation group was increased （P<0.05）. After treated with NaBt alone or combined with 4 Gy X-ray irradiation for 24 h， the mRNA levels of caspase-3 and p21 in the cells in NaBt combined with 4 Gy X-ray irradiation group were significantly higher than those in control group， 4 Gy X-ray irriadiation group and NaBt group （P<0.01）. Compared with control group， the p21 mRNA levels in NaBt group and 4 Gy X-ray irriadiation group were significantly increased （P<0.01）.

NaBt can induce the apoptosis of A549 cells， and its mechanisms may be related to the regulation of mitochondrial apoptosis pathway.

To investigate the changes in the expressions of solute carrier family 7 member 11 （SLC7A11） and glutathione peroxidase 4 （GPX4） in the human periodontal ligament fibroblasts（hPDLFs） under the inflammatory environment stimulated by Porphyromonas gingivalis lipopolysaccharide（P.g-LPS）， and to clarify the possible mechanism of P.g-LPS in inducing periodontitis.

The hPDLFs were extracted and cultured in vitro， and they were identified by immunohistochemical staining. The hPDLFs were divided into control group （0 mg·L^－1 P.g-LPS）， 1 mg·L^－1 P.g-LPS group， and 10 mg·L^－1 P.g-LPS group； the cells were treated for 24 h. Real-time fluorescence quantitative PCR （RT-qPCR） method was used to detect the expression levels of tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6）， interleukin-1β （IL-1β）， SLC7A11， and GPX4 mRNA in the cells in various groups； Western blotting method was used to detect the expression levels of SLC7A11 and GPX4 proteins in the cells in various groups； 2'，7'-dichlorodi-hydrofluorescein diacetate （DCFH-DA） fluorescence probe method was used to detect the levels of reactive oxygen species（ROS） in the cells in various groups.

The results of RT-qPCR method showed that compared with control group， the expression levels of TNF-α， IL-6， and IL-1β mRNA in the cells in 1 and 10 mg·L^－1 P.g-LPS groups were significantly increased（P<0.05）， while the expression levels of SLC7A11 and GPX4 mRNA were significantly decreased（P<0.05）.The results of Western blotting method showed that compared with control group， the expression levels of SLC7A11 and GPX4 proteins in the cells in 1 and 10 mg·L^－1 P.g-LPS groups were decreased（P<0.05）. The results of ROS assay showed that compared with control group， the levels of ROS in the cells in 1 and 10 mg·L^－1 P.g-LPS groups were increased（P<0.05）. Compared with 1 mg·L^－1 P.g-LPS group， the indicators mentioned above in 10 mg·L^－1 P.g-LPSgroup had significant differences.

The expressions of SLC7A11 and GPX4 in the hPDLFs under inflammation are down-regulated， suggesting that the down-regulation of SLC7A11 and GPX4 expressions may be related to the pathogenesis of periodontitis.

To investigate the optimum doping concentrations of zinc（Zn） and nitrogen（N） in co-doping of titanium dioxide nanoparticles（TiO₂NPs） and the inhibitory effect of co-doping of Zn， N and TiO₂NPs（Zn-N-TiO₂NPs） on the Streptococcus mutans， and to clarify its mechanism.

The TiO₂NPs and Zn-N-TiO₂NPs with different doping concentrations were synthesized by sol-hydrothermal method using butyl titanate， zinc nitrate and ammonia as titanium source，Zn source and N source，respectively.X-ray diffraction（XRD），Raman spectra，scanning electron microscope（SEM），UV-visible absorption spectroscope（UV-vis） were used to characterize the nanoparticles in various groups.Cure Rite radiometer was used to characterize the dental light emitting diode curing lamp.The Streptococcus mutans were divided into blank control group，LED light irradiation group，TiO₂NPs group，1%Zn-3%N-TiO₂NPs group（Zn1 group），3%Zn-3%N-TiO₂NPs group（Zn3 group），5%Zn-3%N-TiO₂NPs group（Zn5 group），and 7%Zn-3%N-TiO₂NPs group（Zn7 group）.The nanoparticles in each group were mixed with Streptococcus mutans bacteria solution（the final concentration of nanoparticles was 2 g·L^－1） and were shaken well.The nanoparticles groups and the LED light irradiation group were exposed to dental LED light for 1， 3，and 5 min，the blank control group was treated with culture medium only，and the number of colonies in each group was recorded by plate colony counting method.The group with the best antibacterial effect（Zn3 group） was selected.The reactive oxygen species（ROS） release amount was detected using DPBF probe，and the experiment was divided into 0 min group， 1 min group， 3 min group and 5 min group.A total of 100 μL Zn3-DPBF solution at the concentration of 2 g·L^－1 in each group was selected for dental LED light irradiation for 0， 1， 3 and 5 min， respectively， and the changes in absorption peaks at 410 nm were observed，indicating the ROS release amount.In the experiment exploring the influence of N-acetyl cystethione（NAC） in the inhibitory effect of Zn3 combined with LED light irradiation on Streptococcus mutans，Streptococcus mutans were divided into control group， 5 mmol·L^－1NAC group，Zn3 group and NAC +Zn3 group.After LED light irradiation for 5 min and culture avoiding light for 24 h，plate colony counting method was used to record the number of colonies in each group.

Zn-N-TiO₂NPs was successfully synthesized and its crystal structure was anatase.The average sizes of nanoparticles in TiO₂NPs group，Zn3 group，Zn5 group and Zn7 group were 15.6， 11.3， 9.8， 9.4 and 7.3 nm， respectively.The SEM results showed that the nanoparticles were spherical particles with uniform distribution.The Uv-vis results showed that the absorbance（A） value of Zn3 was increased significantly in the wavelength range of 400－500 nm.Compared with blank control group， the colony count in Zn3 group was the least when LED irradiation was 5 min（P<0.05）.Compared with 0 min group，the absorption peaks of Zn3-DPBF solution at 410 nm in 1 min，3 min and 5 min groups were decreased successively， indicating the ROS relaease amount of nanoparticle solution in various were increased with the prolongation of illumination time.Compared with Zn3 group，the number of colonies in NAC+Zn3 group was significantly increased（P<0.05）.

3%Zn-3%N-TiO₂NPs combined with LED light irradiation can effectively inhibit the growth of the Streptococcus mutans through photocatalysis.

To investigate the improvement effect of Rubus root polysaccharide （RRP） on the pancreatic mitochondrial function in the type 2 diabetes mellitus （T2DM） mice， and to clarify its mechanism.

A total of 70 healthy male C57BL/6J mice were adaptively fed for 1 week， 10 mice were randomly selected as control group， and the remaining 60 mice were used to establish T2DM mouse model according to the method of high-fat diet combined with intraperitoneal injection of low dose streptozotocin （STZ）. Fifty mice were randomly selected from 52 mice that were successfully modeled and divided into model group， low， medium， and high doses of RRP groups， and metformin group； with 10 mice in each group.The mice were administered once a day for 10 weeks； the mice in control group and model group were given equal volumes of normal saline by gavage. During the experiment， the general state of mice was observed every day， the fasting blood glucose （FBG） level was monitored weekly， and oral glucose tolerance test （OGTT） was performed every 4 weeks. After continuous treatment for 10 weeks，the area under time-glucose curve（AUC） of OGTT and the insulin resistance index（HOMA-IR） of the mice in various groups were calculated.The levels of serum fasting insulin （FINS）， C-peptide， and glucagon （GC） and the pancreatic mitochondrial function-related indicators were detected. The pancreas tissue of mice in various groups was obtained，the pancreatic indexes were calculated，and the pathomorphology of pancreas tissue of mice in various was observed by HE staining.

Compared with control group， the mice in model group were apathetic， with rough hair and decreased voluntary activity； compared with model group， the mice in different doses of RRP groups and metformin group had better mental state， with shiny hair and increased voluntary activities. Compared with control group， the body mass of the mice in model group was significantly decreased （P<0.05）， the amount of drinking water and food intake were significantly increased （P<0.05）， and the serum FBG level， AUC of OGTT and HOMA-IR were significantly increased （P<0.05）； compared with model group， the body mass of the mice in medium and high doses of RRP groups was increased significantly （P<0.05）， the amounts of drinking water and food intake were decreased significantly （P<0.05）， and the AUC of OGTT was significantly decreased （P<0.05）， the serum levels of FBG and HOMA-IR of mice in different doses of RRP groups were significantly decreased （P<0.05）. Compared with control group， the serum levels of FINS and C-peptide of the mice in model group were significantly decreased （P<0.05）， the GC level was significantly increased （P<0.05）， and the pancreatic index， respiratory control rate（RCR）， and basal respiratory oxygen consumption and ADP/O were significantly decreased（P<0.05）； compared with model group， the serum FINS and C-peptide levels of the mice in medium and high doses RRP groups and metformin group were significantly increased （P<0.05）， the GC levels were significantly decreased （P<0.05）， ADP/O and RCR were significantly increased （P<0.05）， and the pancreatic indexes and basal respiratory oxygen consumption of the mice in different doses of RRP groups and metformin group were significantly increased （P<0.05）. Compared with control group， the shape of islets in the pancreas tissue of the mice in model group was irregular， and the size and number of islet cells were significantly reduced， accompanying with by inflammatory infiltration and necrosis； compared with model group， the morphology of islets in the pancreas tissue of the mice in different doses of RRP groups was complete， the sizes and number of islets were increased， and the areas of necrosis were decreased.

RRP can improve the function of pancreatic mitochondria and increase the secretion of insulin， thereby regulating the blood glucose of T2DM mice.

To investigate the effects of tumor associated macrophages（TAMs）-derived interleukin-6 （IL-6） on the leukemia inhibitory factor （LIF） expression in oral squamous cell carcinoma （OSCC） Cal27 cells and the invasion and migration of OSCC Cal27 cells，and to clarify their possible mechanisms.

The human OSCC Cal27 cells and mouse peritoneal macrophages（Raw264.7 cells ）were cultured in vitro. The Raw264.7 cells were divided into control and experimental groups. The cells in control group were incubated with DMEM， and the cells in experimental group were incubated with Cal27 conditioned medium （Cal27-CM） to obtain the TAMs. The expression levels of IL-6 mRNA and IL-6 levels in the cells in two groups were detected by real-time fluorescence quantitative PCR （RT-qPCR） and enzyme-linked immunosorbent assay （ELISA） methods. The Cal27 cells were divided into control group and experimental group （5 μg·L^－1 Tocilizumab）.The Cal27 cells in control group were directly treated with TAMs conditioned medium， and the Cal27 cells in experimental group were treated with interleukin 6 receptor （IL-6R） inhibitor Tocilizumab in advance and then treated with TAMs conditioned medium. Transwell chamber experiment was used to detect the migration and invasion abilities of Cal27 cells in two groups. Cell scratch assay was used to detect the scratch healing rates of Cal27 cells in two groups. RT-qPCR and Western blotting methods were used to detect the expression of levels LIF mRNA and protein in Cal27 cells in two groups.

The results of RT-qPCR and ELISA showed that the mRNA expression level of IL-6 and the IL-6 level in the TAMs in experimental group were significantly higher than those in the RAW264.7 cells in control group （P<0.01）. The results of Transwell chamber experiment showed that compared with control group， the number of migration and invasion cells in experimental group was significantly reduced （P<0.01）. The results of cell scratch experiment showed that compared with control group， the scratch healing rate of the Cal27 cells in experimental group was significantly decreased （P<0.01）. The RT-qPCR and Western blotting results showed that compared with control group， the expression levels of LIF mRNA and protein in the Cal27 cells in experimental group were decreased down-regulated （P<0.01）.

TAMs-derived IL-6 promotes the invasion and migration of OSCC Cal27 cells by up-regulating the expression of LIF in tumor cells.

The hDPSCs were extracted and cultured in vitro and identified by flow cytometry. A light-cured HepMA hydrogel was synthesized， and the surface morphology of the material was observed by scanning electron microscope.The HepMA loaded with TGF-β3 （TGF-β-HepMA） at different concentrations （20， 40， 60， 80 and 100 μg?L^－1） was synthesized and its extract was prepared to culture the hDPSCs for 1， 3 and 5 d； meanwhile， control group was established， and the optimal concentration of TGF-β3 loaded was screened out by CCK-8 method. Enzyme-linked immunosorbent assay （ELISA） was used to detect the cumulative release amounts of TGF-β3 in TGF-β-HepMA at different time points and the drug release curve was drawn.The HDPSCs were divided into control group， HepMA group and HE PMA group loaded with the optimal concentration of TGF-β3 （TGF-β3-HepMA）. After being cultured for 24 h in the medium containing the corresponding extracts， the osteogenic induction solution was prepared to induce hHDPSCs for 7， 14 and 21 d， respectively. ALP staining and Alizarin red staining were used to detect the cell ALP staining and calcium nodule formation in each group. Real-time fluorescentce quantitative PCR（RT-qPCR） was used to detect the mRNA expression levels of osteogenic related factors in the cells in each group.

The hDPSCs isolated and cultured were spindle-shaped under light microscope.The flow cytometry results showed positive expressions of CD105 and CD90 and negative expressions of CD34 and CD45 in the cultured hDPSCs， confirming that the cells isolated and cultured in this experiment were the hDPSCs. The scanning electron microscope （SEM） observation showed that the average pore size of HepMA was 50－70 μm. The ELISA results showed that TGF-β3 released slowly after a sudden release phase within 7 d and reached the equilibrium around 21 d.The CCK8 method results showed that compared with 0 μg?L^－1 TGF-β3-HepMA group， the proliferation rate of hDPSCs in 60 μg?L^－1 TGF-β 3-HEPMA group was increased（P<0.05）， so the optimal concentration of TGF-β3 loaded was 60 μg?L^－1. Compared with control group and HepMA group， the ALP staining area in tTGF-β3-HepMA group was the largest and the most stained in color when osteogenesis induction for 7 and 14 d， and the ALP staining in cells on the 14th day was more significant than that on the 7th day. Compared with control group and the HepMA group， the mineralized calcium nodule area stained with Alizarin red was the largest in TGF-β3-HepMA group at 21 d of osteogenic induction. The RT-qPCR results showed that compared with control group， the mRNA expression levels of Runx2， ALP， OCN and COL-Ⅰ in the cells in HepMA and TGF-β3-HepMA groups were significantly increased after 7 and 14 d of culture（P<0.05 or P<0.01）； compared with HepMA group， the mRNA expression levels of Runx2， ALP， OCN and COL-Ⅰ in the cells in TGF-β3-HepMA group were significantly increased （P<0.05）.

HepMA loading TGF-β3 can improve the osteogenic differentiation of hDPSCs， and its mechanism may be related to the up-regulation of the expressions of osteogenic related factors.

The expressions of YTHDF2 protein in 113 cases of ESCC tissue and 95 cases of normal tissue adjacent to esophageal carcinoma were detected by immunohistochemical method. The relationships between the expression of YTHDF2 protein and the clinicopathological features and prognosis of the ESCC patients were analyzed.The Eca109 and EC9706 cells cultured in vitro were divided into control group （transfected with si-NC） and si-YTHDF2 groups （transfected with si-YTHDF2#1 and si-YTHDF2#2）. The expressions of YTHDF2 protein in the cells in various groups were detected by Western blotting method. The proliferation abilities of cells in various groups were detected by CCK-8 method， the number of colony formation in various groups was detected by colony formation experiment， and the number of migration cells in various groups were detected by Transwell chamber assay.

The YTHDF2 protein mainly expressed in the cytoplasm of ESCC cells and a small amount of YTHDF2 protein expressed in the nucleus.The expression amount of YTHDF2 protein in ESCC tissue was significantly higher than that in paracancerous normal tissue （P<0.01）. There were significant differences in the expression intensities of YTHDF2 protein in the ESCC patients with different ages of onset and TNM stages （P=0.008，P=0.041）. The Kaplan-Meier survival analysis showed that compared with YTHDF2 low expression group，the survival time of the patients in YTHDF2 high expression group was significantly shortened（P<0.05）. The CCK-8 method and colony formation experiment results showed that the proliferation activities and the number of colony formation of esophageal carcinoma cells in si-YTHDF2 transfection groups were significantly lower than those in control group （P<0.01）. The Transwell chamber assay results showed that the number of migration esophageal carcinoma cells in si-YTHDF2 transfection groups was significantly lower than that in control group （P<0.01）.

The expression of YTHDF2 in ESCC tissue is significantly higher than that in the normal tissue. YTHDF2 knockdown contributes to inhibiting the proliferation， colony formation and migration of esophageal carcinoma cells.

To investigate the antibiotic resistance，genotyping and biofilm formation ability of panton-valentine leukocidin （pvl） negative Staphyloccocus aureus.

A total of 100 strains of Staphylococcus. aureus were isolated from patients in the Affiliated Hospital of Inner Mongolia Medical University ［methicillin resistant Staphylococcus aureus（MRSA） and methicillin sensitive Staphylococcus aureus（MSSA）］，and the bacterial identification and antibiotic susceptibility test to 12 kinds of antibiotics were performed.The gene typing was performed by adopting accessory gene regulator （agr） typing method.The pvlgene and 31 virulence genes were analyzed by PCR method. The biofilm-forming ability was assessed using the crystal violet staining method.

Among 100 pvl negative Staphylococcus aureus clinical isolates， 38 isolates were MSSA， while 62 isolates were MRSA. The Staphylococcus aureus isolates were all sensitive to vancomycin， linezolid and quinupristin/dalfopristin， but the resistance rates of MRSA to the other antimicrobials were higher than those of MSSA isolates. The agr genotyping results showed that agr-Ⅰ to agr-Ⅳ types were all found among the Staphylococus aureus isolates， and agr-Ⅰ type was found to be the dominant type， accounting for 79.0%， followed by agr-Ⅱ type （16.0%）. The carriage rate of agr-Ⅱ type among MSSA isolates was significantly higher than that of MRSA isolates （81.3% vs 19.7%，P<0.05）. Moreover， the carriage rate of agr-Ⅰ type among MRSA isolates was significantly higher than that of MSSA isolates （72.2% vs 27.8%，P<0.05）. Among 32 virulence genes detected in this study， the positive rates of hla and hld genes were all 100%，while no isolate carried edin， etb and pvl gene. The carrige rates of sdrE， sea， seb， seH， tsst， lukDE， lukM， hlb， mpHLG2-1 among MRSA isolated were higher than those of MSSA isolates， when carrige rates of sea and seh（56.5% and 79.0%） were higher than those of MSSA （7.9 and 60.5%）（P<0.05 or P<0.01）； the carriage rates of the other 18 virulence related genes of MRSA were all lower than those of MSSA isolates， and the carriage rates of mpHLG， seo， sed and sej among MRSA isolates were significantly lower than those of MSSA isolates （P<0.05 or P<0.01）； 23.7% （9/38） MSSA isolates carried at least 15 virulence genes， which was significantly higher that of MRSA isolates［14.5%（9/62）］. Furthermore， the biofilm producing isolates among MSSA and MRSA isolates accounted for 100%（38/38） and 85.5%（53/62） （P<0.05），respectively.

Among 100 pvl-negative Staphylococcus aureus clinical isolates， agr-Ⅰ type is the most predominant. The MRSA isolates show higher prevalence of resistance to multiple antimicrobials， while the carrying rates of virulence genes among MSSA isolates are higher than those of MRSA isolates. The biofilm forming ability of MSSA isolates is higher than that of MRSA isolates.

To explore the influencing factors of recurrence and survival of the patients with cholangiocarcinoma （CCA） after radical resection， and to establish the nomogram to predict the postoperative survival time.

The clinical and pathological data of 89 patients who underwent cholangiocarcinoma resection in our hospital were retrospectively collected. The median time of disease-free survival （DFS） and overall survival （OS） of the patients were 10 and 13 months， respectively. Kaplan Meier and Cox regression models were used to analyze the effects of clinical and pathological characteristics on DFS and OS. LASSO regression was performed by R software to evaluate the related influencing factors of recurrence of the patients after surgery. A nomogram was established to predict the OS of patients after operation， and the prediction efficiency was evaluated by the consistency index （C-index）， calibration curve and area under receiver operaing characteristic （ROC） curve （AUC）.

The postoperative univariate analysis showed that diabetes （χ²=5.204， P=0.023）， CA19-9 （χ²=7.694， P=0.006）， CA125 （χ²=6.908， P=0.009）， CA242 （χ²=10.712， P=0.001）， tumor size （χ²=4.310， P=0.038）， lymph node metastasis （χ²=16.883， P<0.001） were the independent risk factors of DFS after operation in the CCA patients. The Kaplan-Meier survival analysis results showed that CEA （χ²=5.188， P=0.023）， CA19-9 （χ²=9.324， P=0.002）， CA125 （χ²=9.568， P=0.002），CA242 （χ²=19.119， P<0.001）， prealbumin （χ²=4.370， P=0.037）， ALT （χ²=4.072， P=0.045），AST （χ²=6.401， P=0.011）， ALP （χ²=4.682， P=0.045）， lymph node metastasis （χ²=11.739，P<0.001），portal carcinoma （χ²=5.940， P=0.015）， vascular invasion （χ²=4.892， P=0.027）， postoperative adjuvant therapy （χ²=4.011， P=0.045） were the risk factors affecting postoperative survival of the CCA patients. The LASSO regression analysis and COX multivariate analysis showed that lymph node metastasis was an independent risk factor of postoperative recurrence of the patients（OR=3.067，95%CI=1.192－8.252，P=0.022）， lymph node metastasis（HR：2.094，95%CI=1.074－4.083，P=0.030） and postoperative adjuvant therapy（HR=0.374，95%CI=0.190－0.736，P=0.004） were the independent factors of survial of the CCA patients. A nomogram was established to predict the OS of CCA patients， Tte C-index was 0.697， and the AUC of the 1-year， 2-year and 5-year survival rates were 0.72 （95%CI： 0.68－0.88），0.65 （95%CI： 0.63－0.81），and 0.84（95%CI： 0.77－0.91），respectively.

The prognosis of CCA patients is poor and the postoperative survival rate is low. Lymph node metastasis is a common independent risk factor for both recurrence and survival. Combined with postoperative adjuvant therapy， a nomogram is constructed， which has a good predictive efficiency in survival in the CCA patients after radical resection.

To explore the relationships between the serum iodine and iron levels and the thyroid function and thyroid peroxidase antibodies（TPOAb） in the pregnant women during the early pregnancy， and to provide a scientific basis for guiding the individualized iodine and iron supplementation.

A total of 404 pregnant women during early pregnancy in our hospital were enrolled. The general information of patients was collected；the levels of serum ferritin （SF）， haemoglobin （Hb）， free thyroxine （FT4），thyroid-stimulating hormone（TSH），TPOAb， urinary creatinine （UCr） and urinary iodine concentration（UIC） were measured in the pregnant women.The pregnant women were divided into iodine deficiency group（UIC/UCr<150 μg·g^－1），idodine adequate group（150 μg·g^－1 ≤UIC/UCr<250 μg·g^－1）and more-than-adequate/excessive iodine group（UIC/UCr≥250 μg·g^－1） according to the different idodine levels.The pregnant women were divided into iron deficiency group（SF<20 μg·L^－1），iron adequate group（20 μg·L^－1≤SF<150 μg·L^－1） and iron excess group（SF≥150 μg·L^－1） according the different iron levels.The thyroid function，the serum TPOAb positive rates and the prevalence of subclinical hypothyroidism and subclinical hyperthyroidism of the pregnant women in various groups were compared.

The median of TSH， FT4， UIC/UCr and SF of 404 pregnant women were 1.15 mIU·L^－1 （0.62 mIU·L^－1，1.63 mIU·L^－1）， 12.96 pmol·L^－1（12.06 pmol·L^－1，13.96 pmol·L^－1），158.66 μg·g^－1（106.93 μg·L^－1，239.41 μg·L^－1） and 60.93 mg·L^－1（33.86 μg·L^－1，89.61 μg·L^－1）.The serum TSH levels of pregnant women in iodine deficiency group and more-than-adequate/excessive iodine group were higher than that in iodine adequate group （P<0.05）. The TPOAb positive rate in more-than-adequate/excessive iodine group was higher than those in iodine deficiency group and iodine adequate group （P<0.05）； the FT4 levels had no significant differences among various groups （P>0.05）.The serum TSH levels and the TPOAb positive rate of pregnant women in iron deficiency group were higher than those in iron adequate group and iron excess group （P<0.05）. The FT4 level of pregnant women in iron deficiency group was lower than that in iron excess group （P<0.05）. The prevalence of subclinical hypothyroidism in the pregnant women in iodine deficiency group and more-than-adequate/excessive iodine group was higher than that in iodine adequate group （P<0.05）. However， the prevalence of subclinical hypothyroidism in the pregnant women in iron deficiency group was higher than those in iron adequate group and iron excess group（P<0.05）， and the prevalence of subclinical hyperthyroidism in iron excess group was higher than those in iron deficiency group and iron adequate group（P<0.05）. The further correlation analysis results revealed that the UIU/UCr ratio had no correlations with the TSH and FT4 levels of the pregnant women（P>0.05））；the SF level was negatively correlated with the TSH level （r=－0.148，P<0.05）， and positively correlated with the TSH level （r=－0.150，P<0.05）.

The nutritional levels of iron and iodine of the pregnant women in early pregnancy is related to thyroid function and thyroid autoimmunity， and the nutritional condition of iron and iodine should be assessed promptly， the individualized supplement of iodine and iron should be done， at the same time， the excessive iodine supplement should be avoided.

The PTC patients underwent surgical therapy in our hospital were selected；the patients’ clinical data were retrospectively analyzed， including age， sex， side， number， metastatic lymph nodes and location of the PTC patients， and the pathologic data were also analyzed， including location， diameter， number， capsular invasion， peri-tumor fibrosis， psammoma bodies， vascular and nervous invasion. The BRAF V600E mutation detection was performed in PTC tissue， the correlation of cervical lymph node metastasis with the clinical characteristics，pothological feateres and BRAF V600E gene mutation of the PTC patients were analyzed，and Logistic regression analysis was performed to analyze the influencing factors of cervical lymph node metastasis in the PTC patients.

In the 321 PTC patients， 129 patients （40.2%） were found with cervical lymph node metastatasis. BRAF V600E gene mutation were found in 279 （86.9%） patients； there was no correlation of BRAF V600E gene mutation with cervical lymph node metastasis in the PTC patients（P>0.05）.Cervical lymph node metastasis had correlations with age， maximal diameter， multiple tumors in bilateral sides， psammoma bodies and vascular invasion（P<0.05 or P<0.01）. The results of Logistic regression analysis demonstrated that age （OR=0.729，95%CI：0.600－0.885）， maximal tumor diameter （OR=1.796，95%CI：1.326－2.433）， tumor number（OR=1.947，95%CI：1.225－3.096），psammoma bodies （OR=2.578，95%CI：1.037－6.409）， and vascular invasions （OR=8.856，95%CI：1.929－40.656） were the influencing factors of cervical lymph node metastasis in the PTC patients.

The cervical lymph node metastasis of the PTC patients shows the correlations with age， maximal tumor diameter， tumor number， psammoma bodies and vascular invasion， and shows no correlation with BRAF V600E gene mutation.

To investigate the role of interleukin-17A （IL-17A） in the occurrence and development of non-small cell lung cancer （NSCLC） and its regulation on vascular endothelial growth factor （VEGF） through the nuclear factor-κB （NF-κB） signaling pathway， and to elucidate their related mechanisms.

A total of fifty-five postoperative paraffin-embedded specimens of the patients with NSCLC were collected. The expressions of IL-17A and CD31 in normal lung tissue and NSCLC tissue were detected by immunohistochemical staining， the correlations between the positive expression rate of IL-17A and the clinicopathological parameters of NSCLC were analyzed， and the correlation between the expressions of IL-17A and CD31-marked microvessel density （MVD） was analyzed by Pearson correlation analysis. The human lung adenocarcinoma A549 cells at logarithmic growth stage were selected and divided into control group （A549 cells）， exogenous recombinant human IL-17A （rhIL-17A） group， BAY11-7082 （NF-κB signaling pathway inhibitor） group and combination group （rhIL-17A+BAY11-7082）. Western blotting method was performed to detect the expression levels of IL-17 receptor A （IL-17RA）， P65，p-P65 and VEGF proteins in the cells in various groups. The MTT method was used to detect the proliferation abilities of human umbilical vein endothelial cells （HUVECs） in various groups after treated with the A549 cell culture supernatant for 48 h.

The positive expression rate of IL-17A in NSCLC tissue was significantly higher than that in normal lung tissue （P<0.05），the positive expression rate of IL-17A in poor-differentiation group was significantly higher than that in well-moderate differentiation group （P<0.05）， and the expression of IL-17A was positively correlated with the MVD in NSCLC tissue （r=0.329， P<0.05）.Compared with control group， the expression levels of IL-17RA， p-P65 and VEGF proteins in the cells in rhIL-17A group were increased （P<0.05）， and the expression levels of IL-17RA， p-P65 and VEGF proteins in the cells in BAY11-7082 group were decreased （P<0.01）. Compared with rhIL-17A group， the expression levels of IL-17RA， p-P65 and VEGF proteins in the A549 cells in combination group were significantly decreased （P<0.05）. After treated with A549 cell culture supernatant， compared with control group， the proliferation ability of the HUVEC in rhIL-17A group was increased （P<0.05），and the proliferation ability in the cells in BAY11-7082 group was decreased （P<0.05）. Compared with the rhIL-17A group， the proliferation ability of the HUVECs in combination group was significantly decreased （P<0.05）.

IL-17A is highly expressed in poorly differentiated NSCLC cells， and it can promote the proliferation of HUVECs by regulating the expression of VEGF via NF-κB signaling pathway.

To investigate the expression of CC chemokine ligand 20 （CCL20） in liver hepatocellular carcinoma（LIHC） tissue and explore its influence in the prognosis in the patients with LIHC， and to elucidate the mechanism of CCL20 affecting the malignant behavior of LIHC. Methods The mRNA expression levels and clinical data in cancer tissue and para-cancer tissue of the LIHC patients were obtained from The Cancer Genome Atlas （TCGA） and UCSC Xena platforms using Rstudio （4.03） software， and the differences in the CCL20 mRNA expression between the two kinds of tissues were compared，and these results were validated using GEPIA combined with GTEx data. Kaplan-Meier survival analysis and Cox regression analysis methods were used to analyze the relationship between the CCL20 mRNA expression level and the prognosis of LIHC patients. The correlation between the CCL20 expression level and alpha fetoprotein （AFP） level in the LIHC patients was analyzed by Pearson correlation analysis， and the area under receiver operating characteristic （ROC） curve（AUC）， Nomogram and correction curve were used to analyze the correlations between the CCL20 mRNA expression level and the clinical diagnosis and survival prediction in the patients with LIHC. GSEA enrichment analysis of the CCL20 mRNA correlation between the expression level expression in cancer tissue of the LIHC patients was performed using the TCGA database， and the correlation between the expression level of CCL20 mRNA and DNA copy-number alterations（CNA） and DNA methylation level were analyzed.

Compared with para-cancer tissue，the CCL20 mRNA expression level in cancer tissue of the LIHC patients was significantly increased（P<0.01）.The Kaplan-Meier survival analysis results showed that the patients with low expression of CCL20 mRNA had a significantly better prognosis than those with high expression of CCL20 mRNA （HR=1.789， P<0.01）. The Cox univariate and multifactorial regression analysis results showed that the CCL20 mRNA expression level was an independent prognostic factor of the LIHC patients（P<0.05）； the Pearson correlation analysis showed a positive correlation between the CCL20 mRNA expression levels and AFP levels in cancer tissue of the LIHC patients （P<0.01）. The CCL20 mRNA expression level had certain reference value in assessment of clinical diagnosis of the LIHC patients（AUC=0.69，P<0.01）， and could effectively predict the survival rate of the LIHC patients. The GSEA analysis showed that CCL20 could be involved in LIHC cell adhesion， cytokine-cytokine receptor interaction and ribosome function implementation through chemokine， NOD-like receptor（NLR）， PPAR and Toll-like receptor（TLR） tumor-related signaling pathways. The genetic and epigenetic analysis showed that compared with normal diploid sample group， the expression levels of CCL20 mRNA in copy number loss group and copy number amplification group had no significant differences（P>0.05）；compared with copy number loss group，the CCL20 mRNA expression level in copy number amplification group was significantly increased（P<0.05）；the expression level of CCL20 mRNA was negatively correlated with its DNA methylation level （cor=－0.11，P<0.05）.

The CCL20 expression upregulation in LIHC tissue is significantly associated with the poor prognosis of LIHC patients， which can be involved in the malignant process of LIHC through multiple signaling pathways， and it can be used as one of the indicators for prognostic assessment and prediction of survival of the LIHC patients.

To investigate the expression of miR-27a in villus tissue of the patients with recurrent abortion （RM）， and to clarify its effects on trophoblast cell proliferation and apoptosis and their mechanisms.

The villus tissues of 17 RM patients （RM group） and normal pregnant women （healthy control group） were collected. The expression levels of miR-27a in villus tissue of the subjects were detected by real-time fluorescence quantitative PCR （RT-qPCR）. The human chorionic trophoblasts HTR-8/SVneo were cultured in vitro. The miR-27a mimic or inhibitor or negative control sequence （miR-NC） were transfected into the trophoblasts by liposome transient transfection；at the same time，control group（without transfection） was set up. After RT-qPCR method was used to detect the transfection efficiency， CCK-8 method and EdU experiment were used to detect the proliferation activities of the cells and the rates of EdU positive cells in various groups， and flow cytometry was used to detect the percentages of trophoblasts at different cell cycles in various groups； Annexin Ⅴ-FITC/PI method was used to detect the apoptotic rates of cells in various groups. The target genes of miR-27a were analyzed by bioinformatics， Cyclin D1 and insulin-like growth factor 1 （IGF1） were screened for verification， and luciferase target gene reporting experiment， RT-qPCR method and Western blotting method were used to detect the regulatory relationship between miR-27a and Cyclin D1 IGF1； RT-qPCR method and Immunohistochemistry were used to detect the expressions of Cyclin D1 and IGF1 mRNA and proteins in villi tissue of subjects in RM group and healthy control group. Pearson’s correlation analysis was used to analyze the correlation between miR-27a and the expression levels of Cyclin D1 and IGF1 mRNA.

Compared with healthy control group， the expression level of miR-27a in villus tissue of the patients in RM group was increased significantly （P<0.01）.Compared with control group， the expression level of miR-27a in the cells in miR-27a mimic group was significantly increased （P<0.01）， while that in the miR-27a inhibitor group was significantly decreased （P<0.01），and the expression level of miR-27a in the cells in miR-NC group had no significant difference（P>0.05）.Compared with control group， the proliferation activity and percentage of cells in S phase in miR-27a mimic group were decreased significantly（P<0.05）， while the percentage of cells in G₀/G₁ phase and apoptosis rate were increased significantly （P<0.05）；the proliferation activity and percentage of cells in S phase in miR-27a inhibitor group were significantly increased（P<0.05）， and the percentage of cells in G₀/G₁ phase and apoptotic rate were significantly decreased （P<0.05），and the above indicators in miR-NC group had no significant differences（P<0.05）. The results of luciferase target gene reporter assay， RT-qPCR and Western blotting confirmed that miR-27a could targetedly inhibit the expressions of Cyclin D1 and IGF1. Compared with healthy control group， the expression levels of Cyclin D1 and IGF1 mRNA and proteins in villi tissue of the patients in RM group were significantly reduced （P<0.05）， and there were significantly negative correlations between the mRNA expression levels of Cyclin D1 and IGF1 and the expression level of miR-27a in villus tissue of the RM patients （r=－0.214， P<0.05； r=－0.307， P<0.05）.

Abnormally elevated miR-27a in villus tissue of the RM patients may block the cycle progression of trophoblasts， inhibit their proliferation， and promote apoptosis by targeted regulating the expression of Cyclin D1 and IGF1.

To analyze the clinical manifestations and diagnosis and treatment process of a patient with dermatomyositis complicated with Sjogren’s syndrome with coexistence of anti-Jo-1 and anti- SRP antibodies， and to provide reference for the diagnosis and treatment of this rare case.

The clinical materials of the patient with dermatomyositis complicated with Sjogren’s syndrome with coexistence of anti-Jo-1 and anti-SRP antibodies were collected，all kinds of information， including gender， age of onset， clinical manifestations， laboratory data and follow-up information were recorded， and the clinical data were retrospectively analyzed and the related literature were reviewed.

The female patient aged 52 years old was admitted to hospital because of “interstitial pneumonia” due to intermittent cough， expectoration， shortness of breath and general weakness for 2 months. The patient’s hands had mild “mechanic’s hands” like changes， and the limb muscle strength was grade 3. The laboratory examination showed that the creatine kinase level was increased significantly， and anti-Jo-1 antibody and anti-SRP antibody were positive； the imaging results showed the pulmonary interstitial changes；the muscle pathological results showed atrophy， degeneration and inflammatory cell infiltration， and the labial gland pathology showed xerostomia； the clinical diagnosis was DM complicated with SS. After treatment with glucocorticoid combined with mycophenolate mofetil， the condition was improved and the patient was discharged from the hospital. The patient was followed up for 5 months， the creatine kinase level was completely decreased to normal， and the clinical symptoms of DM were significantly improved.

Double myositis-specific autoantibodies positive DM complicated with SS has a low incidence， and its clinical manifestations are atypical and easily involve the lung； the clinical manifestations， pathological examination and muscle zymography test are the mainstays for the diagnosis of this disease， and in this patient glucocorticoids combined with immunosuppressive regimens are effective. The patients should be followed up regularly during treatment process.

To analyze the clinical manifestations， diagnosis and treatment method of the patients with familial multiple odontogenic keratocysts （OKC）， and to improve the clinicians’ awareness of the disease.

The clinical data and follow-up results of 2 cases of OKC patients were collected， the disease was analyzed and the related literatures were reviewed； the diagnosis and treatment progress of multiple OKC related to nevus basal cell carcinoma syndrome（NBCCS） were analyzed Results： Patient 1 was a 32-year-old female， presented with wide orbital space with multiple OKC； the left mandibular bone cyst received curettage， marsupialization and curettage+grinding treatment of the bone wall， respectively； the left maxillary lesion was removed by curettage with grinding treatment of the bone wall before 7 years.and no recurrence was found during follow-up； a recent follow-up showed radiolucency in the right maxilla and mandible， considering the occurrence of new cysts. Patient 2 was the 9-year-old daughter of patient 1； the clinical manifestations were prominent forehead， severe hypertelorism， a large number of melanin nevus on the left canthus and left neck with multiple OKC； the imageological examination was taken when the child was 6 years old and no abnormalities were found in her jaw bone. Before half a month， the radiolucent areas were found in the left and right mandible by panoramic radiography due to routine examination for dental treatment； curettage of cyst of both mandiblar bones was performed， the teeth involved in the cyst 33 and 47 were simultaneously removed，and part of bone was ground outside the cyst；half a month after surgery， the child took follow-up and wound healing was well.

As a systemic disease with familial genetic predisposition， multiple OKC is usually the primary manifestation of NBCCS. Therefore， if multiple OKC patients have been found clinically， it is recommended to screen the possibility of familial multiple OKC and NBCCS， and early diagnosis and treatment should be performed.

To analyze the clinical manifestations， imaging and pathological features， treatment methods and prognosis of pulmonary mucoepidermoid carcinoma，and to provide the basis for the diagnosis and treatment of this disease.

The clinical data of a patient with pulmonary mucoepidermoid carcinoma were collected， and the clinical characteristics， diagnosis and treatment methods of pulmonary mucoepidermoid carcinoma were discussed based on the review of relevant literatures.

The male patient aged 59 years old was admitted to the hospital because of “lung cancer discovered for more than 1 year and fatigue for 1 week”.The physical examination showed bilateral thoracic symmetry， no enhancement of speech tremor in both lungs， clear voice on percussion of both lungs， clear breath sounds on auscultation of both lungs， and no dry or wet rales. Based on the past history， a definitive diagnosis of pulmonary mucoepidermoid carcinoma was made. On admission， the chest CT results showed a mass shadow next to the mediastinum of the right upper lung with reduced density，the CT value was 29－43 Hu， blurred pleural margin， the size was 68 mm × 40 mm， the CT value after enhancement was about 73Hu in arterial phase， and the CT value was about 83 Hu in venous phase. Lymph node shadows were seen in the right hilum and mediastinum. Due to the previous operation， recurrence after chemotherapy， and unable to tolerate continued chemotherapy and targeted therapy， CT-guided argon-helium knife cryoablation was performed for the tumor in the right lung. The chest CT re-examination 1 month after the operation showed that the lesion was significantly reduced and the density was reduced. The value of CT was about 34 Hu， the size was 23 mm×26 mm， no obvious enhancement was seen after enhancement， and the right hilar and mediastinal lymph nodes were also smaller than before.

Pulmonary mucoepidermoid carcinoma is a special type of lung cancer with no obvious positive signs. High-grade pulmonary mucoepideromoid is prone to recurrence after surgery； the chemotherapy effect is poor， and there is no effective targeted drug therapy. Argon-helium knife cryotherapy can locally ablate the lesions， prolong the survival period，and improve the quality of life.

To analyze the efficacy and safety of Del Nido cardioplegic in coronary artery bypass grafting under extracorporeal circulation alone， and to compare the myocardial protection effect of Del Nido cardioplegic and 4∶1 blood containing cardioplegic in coronary artery bypass grafting， and to provide the reference for the selection of cardioplegic.

A total of 51 patients underwent coronary artery bypass grafting under cardiopulmonary bypass in our hospital were selected， including 30 patients received Del Nido cardioplegia （DN group） and 21 patients received blood containing cardioplegia （hemoperfusion group）.The intraoperative aortic cross-clamp （ACC） time， cardiopulmonary bypass （CPB） time， the number and total amount of pleural fluid perfusion， automatic repulse， introoperative blood loss，blood biochemical index，operation time， postoperative mechanical ventilation time and other indicators of the patients in two groups were collected， and the postoperative complications such as atrial fibrillation， myocardial infarction and retrosternal infection of the patients in two groups were observed.

There were no statistical differences in radix data of the patients between DN group and hemoperfusion group （P>0.05）. Compared with hemoperfusion group，the number and volume of intraoperative cardioplegia and intraoperative blood transfusion demand of the patients in DN group were significantly decreased （P<0.01）， and the rate of automatic resuscitation was increased（P<0.01）. There were no significant differences in potassium ion， blood glucose， hemoglobin， lactic acid level， hematocrit， postoperative mechanical ventilation time， time of ICU stay， postoperative creatinine level and postoperative complications of the patients between two groups before and after transfer （P>0.05）.

Del Nido cardioplegia is safe and effective in coronary artery bypass grafting， and has a clear myocardial protection effect. In addition， compared with conventional blood-containing cardioplegia， Del Nido cardioplegia can reduce the total amount and times of cardioplegia， have a higher repulse rate， and reduce the amount of perioperative blood transfusion.

To analyze the clinical efficacy of neodymium yttrium aluminum garnet （Nd∶YAG） laser assisted periodontal regeneration in the treatment of the patients with periodontitis and molar furcation lesions，and to provide basis for the treatment of this disease with ND∶YAG laser.

A total of 76 patients with periodontitis and molar root bifurcation who were treated by periodontal regenerative surgery were selected as the subjects. They were divided into simple periodontal tissue regeneration group （control group） and Nd∶YAG laser irradiation-assisted periodontal tissue regeneration surgery group（experimental group）， with 38 cases in each group. The patients in two groups underwent conventional guided tissue regeneration， and the patients in experimental group received Nd∶YAG laser to treat the root bifurcation area during the operation. The periodontal probing depth （PPD）， vertical attachment loss （CAL-V）， horizontal attachment loss （CAL-H）， bleeding index （BI），and the horizontal penetration depth of root bifurcation （HPD） before surgery， 6 months after surgery and 12 months after surgery of the patients were compared between two groups； the values of vertical bone loss （BL-V） and horizontal bone loss （BL-H） were measured by cone beam CT （CBCT）.

There were no significant differencs in PPD， CAL-V， CAL-H， BI， BL-V and BL-H of the patients between two groups before surgery （P>0.05）；compared with before surgery，PPD， CAL-V， CAL-H， BI， HPD， BL-V， BL-H of the patients in two groups all showed a downward trend at 6 and 12 months after surgery； the above indicators in experimental group at 6 and 12 months after surgery were significantly lower than those in control group （P<0.05）. There was no statistical difference in the chewing efficiency of the patients between two groups before surgery （P>0.05）.Compared with before surgery， at 6 and 12 months after surgery， the chewing efficiencise of the patients in two groups had a increasing trend； the chewing efficiencies in experimenyal group were higher than those in control group at 6 and 12 months after surgery （P<0.01）.

In the periodontal regeneration surgical treatment of the patients with periodontitis and molar root bifurcation lesions， the use of Nd∶YAG laser adjuvant treatment can effectively enhance the treatment effect of periodontal regenerative surgery， reduce the periodontal inflammation，promote the periodontal tissue healing，and improve the chewing function of the patients.

To explore the difficulties and solutions of aesthetic restoration of anterior teeth and clarify the significance of multidisciplinary therapy， and to provide the experience and reference for the diagnosis and treatment of similar difficult cases.

A case of aesthetic restoration in anterior tooth area successfully completed by multidisciplinary therapy after failing bridge crown restoration was selected and followed up for 3 years. Combined with the relevant literature review， the effect of multidisciplinary therapy on the aesthetic restoration of consecutive affected teeth in the anterior area was analyzed from the perspective of “pink and white aesthetics”.

One 30-year-old female patient was admitted due to repeated loss of 12—22 bridge crown. The specialist examination showed that 12—22 tooth crowns were in the state of preparation； the X-ray image showed lamina dura of 12， 21， 22 was the alveolar bone crest - enamel cemental junction was <2 mm， the alveolar bone on the distal side of 11 was absorbed into 1/3 of the neck in angular shape， 11， 21， 22 had secondary caries， and the root canal treatment had been completed； the periodontal probing depth of 11 was 8 mm on the labial side， 5 mm on the distal side， 3 mm on the proximal and palatal side， which presented a narrow and deep bone defects in the two walls of the labial and distal sides， tooth mobility （Degree 3）；the gingival margin of 12， 21， 22 were slight swelling， the periodontal probing depth was <3 mm， tooth mobility （－）. Crown lengthening （12， 21， 22）， extraction （11） and site preservation simultaneously were planned， and 12—22 fixed bridge repair was performed 3 months later. Due to the personal reasons， the patient failed to perform the fixed bridge repair on time， and 11 months later， the patient proposed switching to implant repair 11. The CBCT results showed that the width of alveolar bone in the lip-lingual direction of 11 was<5 mm， and the gingival margin of 12， 21， and 22 were enlarged in the coronal direction and at the same level as the shoulder. Therefore， 12， 21， 22 single-crown restoration after gingivoplasty was planned， implantation were performed in 11 and guided bone regeneration （GBR） was performed simultaneously on its labial side. Finally， the position of the gingival margin was stable and the bone mass was sufficient. The color and shape of 12—22 all-ceramic single crown are similar to natural teeth， and the alveolar ridge fullness was good， the gingival was pink and tough without inflammation， and the gingival margin was continuous conch shape with left and right symmetry.

Multidisciplinary therapy provides an effective solution to the aesthetic repair problem of continuous multiple teeth in the anterior area， which not only better restores the patients’pronunciation and mastication function， but also achieves the satisfactory aesthetic effects.

To investigate the cognitive status of first-degree relatives （FDRs） of the patients with colorectal cancer （CRC） on the CRC screening， and to reveal the problems in the CRC screening.

Forty-nine FDRs aged 40－75 years from the CRC patients who underwent the colorectal cancer surgery in the Department of Colorectal Surgery of our hospital from January 2019 to December 2019 and were pathologically diagnosed as CRC after the surgery were selected by target sampling method.The questionnaire survey was conducted in the FDRs and the survey data were collected to analyze the cognitive status of the FDRs on CRC screening.

Among the FDRs of CRC patients， 21 cases were 40－50 years old and 28 cases were 50－75 years old；27 males and 22 females；31 children， 10 siblings， and 8 parents. Only 26.5% （13/49） of the FDRs were aware of the “increased risk of CRC in FDRs” and only 22.4% （11/49） of participants had “had a colonoscope in the past 5 years”.75.5%（37/49） of the FDRs did not have “medical staff recommended colonoscope over 40 years old”. The awareness rate of “increased risk of CRC for FDRs” and “had a colonoscope in the past 5 years” among the FDRs aged 40－50 years old， living in urban， college degree or above were higher than those aged 51－75 years old，with below college and rural residents. Among the 38 FDRs of CRC patients who had not undergone colonoscope in the past 5 years， the main reasons which affected the FDRs to do the colonoscopy screening were “no physical symptoms， no need to undergo colonoscope”， fear of colonoscope process， and the tedious and time-consuming colonoscope.Of the 38 FDRs of CRC patients，22（57.9%） cases changed their cognition and were willing to undergo colonoscopy after the communication.

The cognition of CRC patients’ FDRs on the CRC screening is seriously insufficient. It is an important measure to increase the CRC screening rate to strengthen the health education of CRC screening with the support of medical insurance policy.