Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (6): 1403-1410.doi: 10.13481/j.1671-587X.20220605
• Research in basic medicine • Previous Articles Next Articles
Donghui LIU,Mingxi ZHANG,Wenliang FU,Xiumei FU,Chengjun SONG(),Zhihong CHEN()
Received:
2022-02-18
Online:
2022-11-28
Published:
2022-12-07
Contact:
Chengjun SONG,Zhihong CHEN
E-mail:songchengjun@126.com;czh1971@126.com
CLC Number:
Donghui LIU,Mingxi ZHANG,Wenliang FU,Xiumei FU,Chengjun SONG,Zhihong CHEN. Effects of sericin on injury of podocytes induced by high glucose and JNK signaling pathway[J].Journal of Jilin University(Medicine Edition), 2022, 48(6): 1403-1410.
Tab.1
Expression levels of Nephrin, MEKK1, MKK4, JNK1, and c-Jun mRNA and proteins in podocytes in various groups"
Group | mRNA | ||||
---|---|---|---|---|---|
Nephrin | MEKK1 | MKK4 | JNK1 | c-Jun | |
Normal control | 1.000±0.000 | 1.000±0.000 | 1.000±0.000 | 1.000±0.000 | 1.000±0.000 |
Hypertonic control | 0.972±0.007 | 1.025±0.011 | 1.032±0.027 | 1.033±0.026 | 1.011±0.006 |
High glucose | 0.551±0.008* | 2.382±0.083* | 2.147±0.015* | 2.845±0.040* | 2.949±0.020* |
Sericin | |||||
Low concentration | 0.661±0.007△ | 1.650±0.051△ | 1.768±0.032△ | 1.847±0.032△ | 1.948±0.021△ |
Medium concentration | 0.746±0.004△# | 1.449±0.013△# | 1.439±0.082△# | 1.628±0.009△# | 1.696±0.012△# |
High concentration | 0.859±0.008△#○ | 1.290±0.037△#○ | 1.361±0.101△#○ | 1.344±0.026△#○ | 1.289±0.009△#○ |
Group | Protein | ||||
Nephrin | MEKK1 | MKK4 | JNK1 | c-Jun | |
Normal control | 1.200±0.097 | 0.692±0.010 | 0.508±0.048 | 3.886±0.055 | 1.629±0.085 |
Hypertonic control | 1.197±0.040 | 0.727±0.029 | 0.498±0.045 | 4.034±0.193 | 1.574±0.065 |
High glucose | 0.537±0.016* | 1.273±0.080* | 1.141±0.092* | 4.840±0.141* | 2.186±0.020* |
Sericin | |||||
Low concentration | 0.688±0.083△ | 1.080±0.086△ | 0.995±0.039△ | 4.566±0.100△ | 1.970±0.020△ |
Medium concentration | 0.803±0.041△# | 0.928±0.025△# | 0.839±0.027△# | 4.230±0.100△# | 1.831±0.032△# |
High concentration | 1.031±0.029△#○ | 0.824±0.053△#○ | 0.382±0.028△#○ | 3.286±0.200△#○ | 1.437±0.075△#○ |
*P<0.05 compared with normal control group;△P<0.05 compared with high glucose group;#P<0.05 compared with low concentration of sericin group;○P<0.05 compared with medium concentration of sericin group. |
Tab.2
Apoptotic rates of podocytes in various groups"
Group | Apoptotic rate |
---|---|
Normal control | 6.900±0.625 |
Hypertonic control | 11.657±1.116* |
High glucose | 37.530±0.488* |
Sericin | |
Low concentration | 28.343±1.029△ |
Medium concentration | 21.167±0.839△# |
High concentration | 16.267±0.451△#○ |
*P<0.05 compared with normal control group;△P<0.05 compared with high glucose group;#P<0.05 compared with low concentration of sericin group;○P<0.05 compared with medium concentration of sericin group. |
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