Objective： To investigate the effect of lateral parabrachial （LPB） chemical lesion on the fever induced by lipopolysaccharide （LPS），and to clarify whether LPB was involved in the fever induced by LPS. Methods Twelve adult male SD rats were randomly divided into control group and LPB-lesion group（n=6）.The rats in LPB-lesion group were bilaterally injected with ibotenic acid into LPB by nuclear injection to induce chemical lession， and the rats in control group were injected with the equal amount of saline into LPB. The rats were survived for at least 1 week and then were implantated with telemetry transmitter for monitoring the body core temperature （T_core） in the abdominal cavity. The T_core and activities of the rats in two groups at different time points after intraperitoneal injection of normal saline or LPS were monitored by radiotelemetry，and the distributions of NeuN and Nissl bodies in LPB of the rats in two groups were detected by immunofluorescence staining and Nissl staining. Results The rats in control group were induced the typically biphasic fever after intraperitoneal injection of LPS （100 μg·kg^－1），and the T_core started to rise 1.5 h after intraperitoneal injection of LPS， firstly reached its peak at 2.5 h （the first phase）， started to rise again at 3.5 h and reached its peak again at 5.5 h （the second phase） after injection of LPS； the rats in LPB-lesion group also had fever reaction after intraperitoneal injection of LPS.At 5.5 h， the T_core was significantly higher than the basic T_core （P<0.05）；compared with control group， the fever reaction of the rats in LPB-lesion group was significantly weakened， and the increasing of T_core at 2.5 and 5.5 h after intraperitoneal injection of LPS was lower than that in control group （P<0.05）.After intraperitoneal injection of normal saline or LPS， the activities of the rats in control group and LPB-lesion group were increased temporarily compared with those before injection， but there were no significant differences between two groups （P>0.05）.A large number of NeuN immunopositive neurons were distributed in LPB of the rats in control group， with large number of Nissl bodies and the shape was big，and the morphology of the cells was complete and the margin was clear； in LPB-lesion group， there were almost no NeuN immunoreactive neurons in the LPB lesion area at the rostral， middle，and caudal levels in the rostrocaudal directions，the neuronal soma was missing， and the Nissl bodies were largely disappeared. Conclusion LPB chemical lession model partially eliminate the LPS-induced fever，indicating that LPB plays a role in the LPS-induced fever.

Objective To investigate the effect of mediodorsal thalamic nucleus（MD） lesions on the electrical activity of the pyramidal neurons in the medial prefrontal cortex （mPFC） of the rats with Parkinson’s disease（PD）， and to clarify the role of MD in the pathophysiological process of PD. Methods The rat model of PD was established by injecting 6-hydroxydopamine（6-OHDA） into the substantia nigra pars compacta （SNc） of the rats，and MD was damaged by intracerebral injection of ibotenic acid（IBO）. A total of 24 male SD rats were divided into normal group， SNc lesion group （PD model group）， MD lesion group （injected with IBO in MD）， and SNc+MD combined lesion group （ipsilateral MD lesion 1 week after SNc lesion），and there were 6 rats in each group. The numbers of dopamine-positive neurons in SNc and ventral tegmental area（VTA） of the rats in various groups were detected by tyrosine hydroxylase immunohistochemical staining，and Nissl staining was used to determine the location of the electrophysiological recording points and extent of the MD lesion. The firing frequencies and coefficients of variation of average interspike interval（ISI），and discharge patterns of pyramidal neurons in mPFC of the rats in various groups were observed. Results After injection with 6-OHDA， the dopamine neurons in the damaged SNc of the rats in PD model was completely disappeared， and the number of dopaminergic neurons in ipsilateral VTA in the contralateral side was significantly decreased （P<0.01）； compared with normal group， the firing frequency of mPFC pyramidal neurons of the rats in SNc lesion group was significantly increased （P<0.05）， the firing pattern tended to bursting activity （P=0.001）， and the coefficient of variation of average ISI was increased （P<0.01）； two weeks after MD lesion， the firing frequency of mPFC pyramidal neurons of the rats in SNc lesion group was significantly higher than that in normal group （P<0.01）， the bursting firing was increased （P<0.01）， and the coefficient of variation of average ISI was increased （P<0.01）；compared with normal group，the firing frequency of mPFC pyramidal neurons of the rats in SNc+MD combined lesion group had no significant difference（P>0.05），the bursting firing was increased （P<0.01）， and the coefficient of variation of avarage ISI was increased （P<0.01）. Conclusion MD plays an important role in regulating the activity of mPFC neurons， and the change of MD function may be one of the important reasons for the change of mPFC neural activity in the pathophysiological process of PD.

Objective To compare the changes of virulence of Enterobacter cloacae before and after blaNDM-1 gene knockout， and to clarify the effect of blaNDM-1 gene on the virulence of Enterobacter cloacae. Methods The Enterobacter cloacae carrying blaNDM-1 gene were divided into Enterobacter cloacae T2 carrying drug resistant gene blaNDM-1 group（T2 group），Enterobacter cloacae T2 with blaNDM-1 gene-knockout group （ΔT2 group） and Enterobacter cloacae ATCC13047 control group （ST group）. The stabilities of the plasmids in various groups were detected，and the colony movement diameters，biofilm absorbance （A）values， amounts of adhesion bacteria， adhesion rates and invasion adhesion ratios of the Enterobacter cloacae in various groups were detected. Results After 200 passages， the Enterobacter cloacae in T2 group still carried blaNDM-1 gene， while the Enterobacter cloacae in Δ T2 and ATCC13047 groups did not carry blaNDM-1 gene；the Enterobacter cloacae inT2 group only carried the carbapenem resistance gene blaNDM-1， while the other carbapenem resistance genes blaKPC-2， blaIMP-4， blaVIM-1 and blaOXA-48 were negative； five carbapenem resistance genes detected in Δ T2 and ST groups were negative；the Enterobacter cloacae in T2 and Δ T2 groups carried the same virulence genes clpB， icmf and acrA. The colony movement diameter of the bacteria in T2 group was smaller than those in Δ T2 and ST groups （P<0.05）. Compared with T2 group， the biofilm A value in Δ T2 group was increased，but the difference was not significant（P>0.05）. Compared with T2 group， the amount of adhesion bacteria in Δ T2 group was decreased，but the difference was not significant（P>0.05）. After co-culture for 24 h， the adhesion rate and invasion adhesion ratio of the RAW264.7 cells in T2 group and Δ T2 group were lower than that in ST group （P<0.05）. Conclusion BlaNDM-1 does not significantly increase the virulence of Enterobacter cloacae.

Objective To explore the effect of the Jianpi Huayu Qutan method on the dyslipidemia in the rats and to elucidate its molecular mechanism based on apolipoprotein A-Ⅰ（apoA-Ⅰ）/ adenosine monophosphate activated protein kinase （AMPK） / carnitine palmitoyltransferase Ⅰ A（CPT1A） signaling pathway. Methods A total of 32 rats were randomly divided into blank control group， model group， simvastatin group （given 1.575 mg·kg^－1·d^－1 simvastatin） and Huayu Qutan Prescription（HYQTP） group （given 13.846 mg·kg^－1·d^－1 HYQTP drug）， and there were 8 rats in each group. The rats in blank control group were given normal diet，and the rats in model group， simvastatin group and HYQTP group were given high-fat diet to establish the hyperlipidemia rat models. After 8 weeks， the rats in simvastatin group and HYQTP group were given drugs by gavage； the rats in blank control group and model group were given the same volume of normal saline. The levels of total cholesterol （TC）， triglycerides （TG）， low density lipoprotein cholesterol （LDL-c） and high density lipoprotein cholesterol （HDL-c） in serum of the rats in various groups were detected by automatic biochemical analyzer；HE staining was used to observe the pathomorphology of liver tissue of the rats in various groups； Oil red O staining was used to observe the lipid deposition in liver tissue of the rats in various group； the levels of TG in liver tissue of the rats in various groups were detected；ELISA method was used to detect the levels of apoA-Ⅰ in liver tissue of the rats in various groups；real-time fluorescence quantitative PCR （RT-qPCR） method was used to detect the expression levels of apoA-Ⅰ， scavenger receptor class B type 1 （SR-B1）， and CPT1A mRNA in liver tissue of the rats in various groups；Western blotting method was used to detect the expression levels of SR-B1， phosphorylated AMPK （p-AMPK）and CPT1A proteins in liver tissue of the rats in various groups. Results Compared with blank control group， the levels of serum TC， TG，and LDL-c of the rats in model group were increased （P<0.05）， and the level of serum HDL-c was decreased （P<0.05）； the level of TG in liver tissue of the rats was increased （P<0.05）； the hepatocytes showed obvious swelling and increased volume， and the obvious red lipid droplet distribution could be seen； the expression levels of apoA-Ⅰ，SR-B1， and CPT1A mRNA and protein were decreased （P<0.05）， and the ratio of p-AMPK/AMPK was decreased （P<0.05）；compared with model group， the levels of serum TC， TG，and LDL-c in liver tissue of the rats in simvastatin group and HYQTP group were decreased （P<0.05）， and the level of HDL-c was increased （P<0.05），the level of TG in liver tissue was decreased （P<0.05）；the swelling of hepatocytes was obviously reduced，and the distribution of lipid droplets was decreased obviously； the expression levels of apoA-Ⅰ， SR-B1， and CPT1A mRNA and proteins were increased （P<0.05）， and the ratio of p-AMPK/AMPK was increased （P<0.05）. Conclusion The Jianpi Huayu Qutan method can improve the dyslipidemia and reduce the liver damage and lipid deposition of the hyperlipidemia rats， and its mechanism may be related to regulating the apoA-Ⅰ/AMPK/CPT1A signaling pathway and promoting fatty acid β oxidation in the rats.

Objective： To investigate the effects of sericin on the expressions of c-Jun N-terminal kinase（JNK） signaling pathway-related proteins and of apoptosis of podocytes in the podocytes injury inuced by high glucose，and to clarify the protective effect of sericin and its possible mechanism. Methods The podocytes of immortalized mice with mature differentiation were randomly divided into normal control group（medium containing 5.5 mmol·L^－1glucose）， hypertonic control group（medium containing 30.0 mmol·L^－1 glucose and 24.5 mmol·L^－1mannitol）， high glucose group（medium containing 30.0 mmol·L^－1 glucose）， low concentration of sericin group（medium containing 30.0 mmol·L^－1glucose and 150.0 mg·L^－1sericin）， medium concentration of sericin group（medium containing 30.0 mmol·L^－1 glucose and 300.0 mg·L^－1 sericin），and high concentration of sericin group（medium containing 30.0 mmol·L^－1 glucose and 600.0 mg·L^－1 sericin）.The morphology of podocytes in various groups was observed under optical inverted microscope， real-time fluorescence quantitative PCR（RT-qPCR） method was used to detect the expression levels of podocyte membrane protein（Nephrin），mitogen-activated protein kinase kinase 1（MEKK1）， mitogen-activated protein kinase kinase 4（MKK4），c-Jun N-terminal kinase 1（JNK1） and c-Jun mRNA in the podocytes in various groups，Western blotting method was used to detect the expression levels of Nephrin， MEKK1， MKK4， JNK1， and c-Jun proteins in the podocytes in various groups，and AnnexinⅤ/PI double staining method was used to detect the apoptotic rates of the podocytes in various groups. Results The podocytes in normal control group had larger cell bodies and extended to produce dendritic foot process；compared with normal control group， the body of the podocytes in high glucose group was significantly smaller， the cell spacing was increased， and the number of detached and suspended podocytes was increased；compared with high glucose group， the morphology of the podocytes in different concentrations of sericin groups were gradually tended to be normal.Compared with normal control group，the expression levels of Nephrin mRNA and protein in the podocytes in high glucose group were obviously decreased（P<0.05）， indicating that the podocyte injury model induced by high glucose was successfully established.Compared with normal control group，the expression levels of MEKK1，MKK4，JNK1， and c-Jun mRNA and proteins in the podocytes in high glucose group were significantly increased（P<0.05）.compared with high glucose group，the expression levels of MEKK1， MKK4， JNK1， and c-Jun mRNA and proteins in the podocytes in different concentrations of sericin groups were significantly decreased（P<0.05）；at the same time， the expression levels of MEKK1， MKK4， JNK1， and c-Jun mRNA and proteins in the podocytes in high concentration of sericin group were significantly lower than those in medium concentration of sericin group （P<0.05）， and the expression levels of MEKK1， MKK4， JNK1， and c-Jun mRNA and proteins in the podocytes in medium concentration of sericin group were significantly lower than those in low concentration of sericin group （P<0.05）.Compared with normal control group，the apoptotic rate of the podocytes in high glucose group and hypertonic control group were signifiantly increased（P<0.05）；the apoptotic rates of the podocytes in different concentrations of sericin groups were significantly lower than that in high concentration of sericin group （P<0.05）， and the apoptotic rate of the podocytes in high concentration of sericin group was significantly lower than that in medium concentration of sericin group （P<0.05）， while the apoptotic rate of the podocytes in medium concentration of sericin group was significantly lower than that in low concentration of sericin group （P<0.05）. Conclusion Sericin has protective effect on the podocyte injury induced by high glucose，and its mechanism may be related to its inhibition of the expressions of MEKK1， MKK4， JNK1， and c-Jun in the JNK signaling pathway and its inhibition of the apoptosis of the podocytes.

Objective To investigate the improvement effect of 1，25-dihydroxycholecalciferol［1，25（OH）₂D₃］ combined with astragalus polysaccharide （APS ）on the insulin resistance（IR） in the skeletal muscle cells and its mechanism， and to provide the basis for alleviating IR by 1，25（OH）₂D₃ and APS. Methods CCK-8 method and hexokinase method were used to determine the optimal dose and duration of PA （0.2， 0.4， 0.6， 0.8， and 1.0 mmol·L^－1），the optimal dose of APS （25， 50， 100， and 200 mg·L^－1），and the optimal dose of 1，25（OH）₂D₃ （1， 10， 100，and 1 000 nmol·L^－1）.The skeletal muscle cells were divided into control group （without any treatment）， PA group （given 0.4 mmol·L^－1 PA for 24 h）， PA+APS group （given 0.4 mmol·L^－1 PA for 24 h and 100 mg·L^－1 APS for 24 h）， PA+1，25（OH）₂D₃ group ［given 0.4 mmol·L^－1 PA for 24 h and 100 nmol·L^－1 1，25（OH）₂D₃ for 24 h］， and PA+APS+1，25（OH）₂D₃ group （given 0.4 mmol·L^－1 PA for 24 h and then 100 mg·L^－1APS and 100 nmol·L^－1 1，25（OH）₂D₃ for 24 h）.The levels of interleukin-6 （IL-6）， interleukin-10 （IL-10）， monocyte chemoattractant protein-1 （MCP-1），and tumor necrosis factor-α （TNF-α） in the cell culture supernatant in various groups were detected by enzyme-linked immunosorbent assay （ELISA） method， and the levels of intracellular reactive oxygen species （ROS） in the cells in various groups were detected by flow cytometry.Western blotting method was used to detect the expression levels of insulin receptor（InsR）， insulin receptor substrate 1 （IRS-1）， phosphorylated insulin receptor substrate 1 （p-IRS-1）， glucose transporter 4 （GLUT4），P65， phosphorylated P65 （p-P65）， P38 mitogen-activated protein kinase （p38MAPK），and Toll-like receptor 4 （TLR4） proteins in the cells in various groups. Results Compared with control group， the levels of ROS， IL-6， TNF-α， MCP-1，and the expression levels of P65， p-P65， p38MAPK， and TLR4 in the skeletal muscle cells in PA group were increased （P<0.05）， and the levels of IL-10，and the expression levels of InsR， IRS-1， p-IRS-1，and GLUT4 proteins were decreased （P<0.05）；compared with PA group，the levels of ROS， IL-6， MCP-1， TNF-α，and the expression levels of P65， p-P65， p38MAPK，and TLR4 proteins in the skeletal muscle cells in PA+APS，PA+1，25（OH）₂D₃ and PA+APS+1，25（OH）₂D₃ groups were decreased （P<0.05），and the levels of IL-10， and the expression levels of IRS-1， p-IRS-1 and GLUT4 proteins were increased （P<0.05）. The level of ROS， and the expression levels of p-P65 and p38MAPK proteins in the skeletal muscle cells in PA+APS+1，25（OH）₂D₃ group were lower than those in PA+APS and PA+1，25（OH）₂D₃ groups （P<0.05），and the expression levels of InsR， IRS-1， p-IRS-1 and GLUT4 protein were higher than those in PA+APS and PA+1，25（OH）₂D₃ group （P<0.05）. Conclusion 1，25（OH）₂D₃ in combination with APS can effectively reduce the IR， which may be achieved by inhibiting the release of oxidative stress pathways and inflammatory factors，and the the effect of combined application is better than that of single application.

Objective To compare the cutting abilities and shaping abilities of three kinds of single formed nickel-titanium instruments for the simulated curved root canals， and to provide the basis for their clinical applications. Methods A total of 40 single curved resin simulated root canals were selected and randomly divided into Reciproc Blue group， WaveOne Gold group， M3-L group， and ProTaper Gold group， and there were 10 single curved resin simulated root canals in each group. The simulated root canals in each group were prepared according to the user manuals， and the preparation time， difference in weights of simulated root canals before and after preparation， difference of bending angles of simulated root canals，apical deviations， removal amounts of internal and external resin， centering ratios of simulated root canals in various groups were recorded. Results The preparation time of the simulated root canals in WaveOne Gold group was shorter than those in the other three groups （P<0.05），and the preparation time of the simulated root canals in ProTaper Gold group was longer than those in the other groups（P<0.05）. There was no significant difference in wieight of the simulated root canals among four groups before and after preparation （P>0.05）.The differences of bending angles of the simulated root canals in M3-L group before and after preparation was smaller than those in the other three groups （P<0.05）.After preparation， the removal amount of internal resin of the apical zone （1－4 mm） was larger than extermal resin（P<0.05）， and the removal amount of external resin of the middle zone （5－8 mm） was larger than internal resin（P<0.05）； at the positions 1， 3 and 6 mm from the apical foramen， the differences between the centering ratios of the simulated root canal in ProTaper Gold group and 1 was the largest， which was larger than those in the other three groups （P<0.05），and the differences between the centering ratio of the simulated root canals and 1 at each observation point in the other groups were not statistically significant （P>0.05）. Conclusion Reciproc Blue， WaveOne Gold and M3-L single formed nickel-titanium instruments can keep the shape of root canals better， and have better preparation efficiencies and center positioning ability than the ProTaper Gold multi-file system. Domestic single nickel-titanium instrument M3-L can better maintain the original curvature of the root canals.

Objective To prepare the novel fluoride loaded poly （prolylene carbonate）（PPC）/sodium fluoride （NaF） dental patch based on PPC and explore its remineralization property and in vitro cytotoxicity， and to clarify its effect of fluorine-containing patches with different concentrations of fluoride in promoting the remineralization of demineralized enamel， and to provide the reference for its clinical application. Methods The PPC/NaF dental patch with different mass fractions of sodium fluoride （0， 0.5%， 2.5%，and 5.0%） were prepared by melt-blending method. The enamel blocks were prepared with extracorporeal tooth and randomly divided into PPC group， 0.5%PPC/NaF group， 2.5%PPC/NaF group， and 5.0%PPC/NaF group according to different contents of fluoride in the dental patches， and there were 10 samples in each group. The morphology of surface of enamel specimens was observed under scanning electron microscope（SEM），the microhardness of the enamel surface before and after demineralization and after remineralization was measured by microhardness tester， and the microhardness recovery percentage （SMHR） was calculated. The mouse fibroblast L929 cells were co-cultured with the PPC/NaF dental patch extract，and CCK-8 test was used to detect the relative proliferation rate （RGR） of the fibroblast L929 cells of the mice in various groups， and its cytotoxicity level was evaluated. Results The microhardness of the enamel specimens in various groups after demineralization was significantly lower than that before demineralization （P<0.05）. After being treated with PPC/NaF tooth patches with different contents of sodium fluoride， the microhardness of the enamel specimens in various groups was significantly higher than that before remineralization（P<0.05）， but still lower than that before demineralization （P<0.05）. The SMHR of enamel specimens in each group from high to low was 5.0% PPC/NaF group， 2.5% PPC/NaF group， 0.5% PPC/NaF group， and PPC group. The SEM results showed that the number of pores on the surface of the enamel specimens in PPC group was still large， and the irregular granular sediments were seen； in 0.5%PPC/NaF group， the number of pores on the enamel surface was decreased significantly，and the obvious enamel collapse was still seen on the surface and mineral crystal deposition was loose and uneven； in 2.5% PPC/NaF group， there were almost no pores on the enamel surface，and the enamel surface was regular and covered with a large number of needle-like crystals， which were densely arranged； in 5.0% PPC/NaF group， the enamel surface was covered with a large number of needle-like or irregular granular crystals and layered sediments were seen in some areas. Compared with PPC group， the RGR of the fibroblast L929 cells of the mice in 5.0% PPC/NaF group was decreased significantly （P<0.05）. The RGR of the fibroblast L929 cells after treated with PPC/NaF tooth patches with different contents of fluoride in various groups was greater than 75%， and the cytotoxicity grade was 0 or 1. Conclusion PPC/NaF dental patch can effectively promote the demineralization of the demineralized enamel without cytotoxicity；with the increasing of fluorine concentration， its remineralization is also enhanced.

Objective To investigate the protective effect of Shenhong Buxue Granules （SBG） on vascular endothelium of the mice with vascular endothelial dysfunction （VED） of Qi stagnation and blood stasis type and its effect on the expression levels of Toll-like receptor 4 （TLR4），myeloid differentiation factor （MyD88），nuclear factor κB p65 （NF-κB p65），intercellular adhesion molecule-1 （ICAM-1） proteins in the human umbilical vein endothelial cells （HUVECs），and to elucidate its relevant action mechanism. Methods A total of 60 Kunming mice were randomly divided into normal control group， VED group， positive control group （0.62 g·kg^－1·d^－1 Xuefuzhuyu Capsule）， low dose of SBG （3 g·kg^－1·d^－1） group， medium dose of SBG （6 g·kg^－1·d^－1） group and high dose of SBG （9 g·kg^－1·d^－1） group，and there were 10 mice in each group. Except for normal control group， the mice in other groups were used to construct the VED models of Qi stagnation and blood stasis type with ice water bath method，and the mice were continuously administered for 21 d. The behavior of the mice in various groups were observed，the whole blood viscosities of the mice in various groups were measured by blood rheometer，HE staining was used to observe the pathomorphology of lung and thoracic aorta tissues of the mice in various groups，the serum levels of von Willebrand factor （vWF）， thrombomodulin （TM），ICAM-1， and tumor necrosis factor-α （TNF-α），and interleukin-6 （IL-6） of the mice in various groups were detected by ELISA method， and the kits were used to measure the levels of nitric oxide （NO） in serum and the activities of glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） in thoracic aorta tissue of the mice in various groups.The human umbilical vein endothelial cells （HUVECs） were cultured in vitro and divided into normal control group， tert-butyl hydroperoxide （TBHP） group and low，middle and high doses of SBG groups.Except for normal control group， the HUVECs in the other 4 groups were induced by TBHP to establish the oxidative damage model of HUVECs. The survival rates of HUVECs in various groups were detected by MTT assay， Western blotting method was used to detect the expression levels of TLR4， MyD88， NF-κB p65， and ICAM-1 proteins in the HUVECs in various groups. Results Compared with normal control group， the grasping force value and the number of autonomous activities of the mice in VED group were significantly decreased （P<0.05），and the whole blood viscosity was increased significantly （P<0.05）； compared with VED group， the grasping force values of the mice in positive control group and the low， middle and high doses of SBG groups were significantly increased （P<0.05）， the whole blood viscosities were significantly decreased （P<0.05），and the numbers of autonomous activities of the mice in positive control group and high dose of SBG group were significantly increased （P<0.05）.The HE staining results showed that compared with normal control group， the lung tissue of the mice in VED group showed obvious telangiectasia and congestion， and the tissue was accompanied by a large number of inflammatory cell infiltration； compared with VED group， the degrees of telangiectasia and congestion of the alveolar wall of the mice in positive control group and different doses of SBG groups were decreased， and the number of inflammatory cell infiltration was significantly decreased in a dose-dependent manner； compared with normal control group， the inner wall of thoracic aorta of the mice in VED group was disordered， with defects and falling off； compared with VED group， the falling off lesion of vascular intima of the mice in positive control group and different doses of SBG groups was improved and the integrity of the intima was maintained. Compared with normal control group， the serum levels of vWF，TM，ICAM-1， TNF-α ，and IL-6 of the mice in VED group were significantly increased （P<0.05）， and the serum level of NO and serum activities of GSH-Px and SOD in aorta tissue were significantly decreased （P<0.05）； compared with VED group， the serum levels of vWF， TM， ICAM-1， TNF-α and IL-6 of the mice in positive control group and the high dose of SBG group were significantly decreased （P<0.05），and the serum level of NO and activities of GSH-Px and SOD in aorta tissue were significantly increased （P<0.05）. Compared with normal control group， the survival rate of HUVECs in TBHP group was significantly decreased （P<0.05），and the expression levels of TLR4， MyD88， NF-κB p65， and ICAM-1 proteins in the HUVECs in TBHP group were significantly increased （P<0.05）； compared with TBHP group， the survival rate of the HUVECs in high dose of SBG group was significantly increased （P<0.05），and the expression levels of TLR4， MyD88， NF-κB p65， and ICAM-1 proteins in the HUVECs in high dose of SBG group were significantly decreased （P<0.05）. Conclusion SBG can protect the vascular endothelium of the mice with VED of Qi stagnation and blood stasis type by inhibiting the oxidative stress and alleviating the inflammatory response.

Objective To discuss the technological process of platelet rich fibrin （PRF）/hydroxyapatite （HA） complex prepared by the “sandwich” method， and to evaluate its properties. Methods The injectable PRF （I-PRF） and advanced PRF （A-PRF） were prepared with the centrifugation indexes of 700 r·min^－1×3 min and 1 300 r·min^－1×14 min，respectively， then they were combined with HA using the “sandwich” method. The microstructure of PRF/HA complex was observed under scanning electron microscope （SEM） after freeze drying； the material was immersed in the simulated body fluid （SBF）， and the growth of a bone-like layer on the surface of HA was observed on the 2nd， 4th， 8th，and 16th days， respectively，and its mineralization ability was evaluated；the PRF/HA complex extract solution co-cultured with preosteoblast MC3T3-E1 cells of the mice and complete culture medium were used as experimental group and control group；after cultured for 1， 3 and 5 d， respectively， the proliferation activities of the preosteoblast MC3T3-E1 cells were evaluated by CCK-8 method. Two full-thickness cranial defect areas with a diameter of 6 mm were symmetrically established on both the sides of the sagittal suture in the Japanese white rabbits； one side implanted with PRF/HA complex was regarded as experimental group， the other side without any material implantion was regarded as control group；X-ray and cone beam CT （CBCT） were used on the surgical area to evaluate the bone formation performance of the rabbits in two groups 4 and 8 weeks after surgery. Results The PRF/HA complex with a “sandwich” structure was successfully prepared， with the A-PRF on the outside and the I-PRF mixed with HA particles on the inside. The SEM observation results showed that the cross-section of the complex was an obvious three-layer structure， with the dense fibrin net on the outside and the porous HA particles attached by fibrin filaments on the inside. After being immersed in SBF，the mineralized nodules were formed on the surface of HA on the 2nd day， the bone-like layer was gradually established with the prolongation of the immersion time， and a lamellar structure was formed at the 16th day. The cell proliferation experiment results showed that compared with control group， the proliferation activities of the MC3T3-E1 cells in experimental group were significantly increased on the 1st， 3rd，and 5th days （P<0.05）. The imaging observation results showed that the cranial defect in experimental group had obvious marginal osteogenesis 4 weeks after surgery and the new bone had almost completely filled the bone defect area 8 weeks after surgery， while the new bone formation of the cranial defect in control group was significantly decreased than that in experimental group. Conclusion PRF/HA complex prepared by the “sandwich” method has a good mineralization ability and biocompatibility， and can promote the bone regeneration.

Methods The pmiRFP670-N1 plasmid and pET-28a plasmid were doubly digested with restriction endonucleases EcoR Ⅰ and Not Ⅰ to construct the pET-miRFP670 prokaryotic expression vector. The LyP-1 DNA sequence was introduced through point mutation to construct the recombinant expression vector pET-miRFP670-LyP1； the recombinant expression vector with correct sequence was transformed into the E.coli BL21 cells and the prokaryotic expression amounts of fusion proteins induced under different temperatures （16 ℃ and 37 ℃） and different concentrations of isopropyl-β-D-thiogalactopyranoside （IPTG）（0.1，0.5，and 1.0 mmol·L^－1） were detected by SDS-PAG electrophoresis； the fusion protein was purified by Ni-NTA resin affinity， and the prokaryotic expression amount of the miRFP670-LyP1 protein was detected； the endocytosis morphology of the miRFP670-LyP1 fusion protein in breast cancer 4T1 cells was observed under fluorescence microscope. Results The double digestion of recombinant plasmid showed that two DNA bands of about 5 343 and 973 bp were obtained， which was consistent with the sizes of the pET-28a vector and miRFP670 gene fragment. The DNA sequencing results showed that the LyP1 sequence was successfully inserted into the pET-miRFP670 expression vector. The soluble protein expression amount of miRFP670-LyP1 fusion protein was higher at 16 ℃ than that at 37 ℃. The miRFP670-LyP1 fusion protein with high purity was obtained by purification with Ni-NTA resin. The fluorescence imaging results showed that the miRFP670-LyP1 fusion protein could be efficiently endocytosed by the breast cancer 4T1 cells. Conclusion The prokaryotic expression vector pET-miRFP670-LyP1 is successfully constructed，the soluble protein expression amount of the fusion protein is higher at low temperature （16 ℃） than that at normal temperature （37 ℃）， and the fusion protein with high purity is obtained by affinity chromatography，and the fusion protein is efficiently endocytosed by the breast cancer 4T1 cells and displays near-infrared fluorescence. Objective To construct the prokaryotic expression vector of tumor homing peptide（THPs）-near-infrared fluorescent protein（NIRFP）-miRFP670 LyP1 fusion protein， and to purified fusion protein， and to investigate the near infrared fluorescence characteristics of the fusion protein.

Objective To investigate the effect of lncRNA-MIAT on M2-type polarization of tumor-associated macrophages， and to elucidate its possible mechanism. Methods The THP-1 cells were cultured in vitro. The lentivirus particles， which over expressed （LV-MIAT） and down regulated expression of lncRNA-MIAT（LV-shMIAT），and empty vector（LV-Vector） were transfected into the THP-1 cells. The THP-1 cells were activated into the macrophages， and the activated macrophages were co-cultured with the osteosarcoma （OS） MG63 cells by using Transwell co-culture system.The above co-culture systems were divided into MG63+LV-Vector group （positive control group），MG63+LV-shMIAT group， MG63+LV-MIAT group，and IL-4+LV-Vector group. The expression levels of lncRNA-MIAT in the THP-1 cells in various groups were detected；the percentages of M2-type macrophages in various groups were detected by flow cytometry； the levels of vascular endothelial growth factor （VEGF）， interleukin-10（IL-10） ，and transforming growth factor-β1（TGF-β1） in supernatant of the THP-1 cells in various groups were detected by ELISA method；the macrophages in each culture system were co-cultured with the human umbilical vein endothelial cell （HUVEC）； EDU staining was used to detect the proliferation activities of the HUVEC in various groups；the numbers of angiogenesis in the HUVEC in various groups were detected by tube formation assay；the expression levels of Janus kinase 1 （JAK1），signal transducer and activator of transcription 6 （STAT6），and phosphorylated STAT6 proteins in the THP1 cells and the expression levels of vascular endothelial growth factor receptor 2（VEGFR2），Notch1，and delta like protein 4（DLL4）in the HUVEC in various groups were detected by Western blotting method；the p-STAT6/STAT6 ratio was calculated.The OS tumor-bearing mouse model was constructed， and 36 nude mice were divided into LV-Vector group， LV-shMIAT group，and LV-MIAT group（n=12）.The volumes and weights of the tumor and the positive expression rates of CD163 and CD31 in tumor tissue of the mice in various groups were detected after interfering lncRNA-MIAT expression. Results The THP-1 cells with stable over-expression or down-regulation of lncRNA-MIAT were successfully established by lentivirus infection. Compared with LV-Vector group， the expression level of lncRNA MIAT in the cells in LV-shMIAT group was significantly decreased （P<0.05）， and the expression level of lncRNA MIAT in the cells in LV-MIAT group was significantly increased （P<0.05）. Compared with MG63+LV-Vector group， the levels of VEGF， IL-10，and TGF- β1 in the THP-1 cells in MG63+LV-shMIAT group were significantly decreased （P<0.05），and the expression levels of VEGFR2， Notch1， and DLL4 proteins in the HUVEC were significantly decreased （P<0.05），the levels of VEGF，IL-10，and TNF-β1 in the THP-1 cells in MG63+LV-MIAT and IL-4+LV-Vector groups were increased significantly （P<0.05 or P<0.01）；the expression levels of VEGFR2， Notch1，and DLL4 proteins in HUVEC were increased（P<0.05）， the percentage of M2-type macrophages，and p-STAT6/STAT6 ratio and expression level of JAK1 protein in the THP-1 cells were increased（P<0.05）；compared with MG63+sh-MIAT group， the VEGF level in the THP-1 cells in MG63+LV MIAT group was decreased significantly （P<0.05）， the levels of IL-10 and TGF-β were decreased significantly （P<0.05），the expression levels of VEGFR2， Notch1，and DLL4 proteins in the HUVEC were increased significantly （P<0.05）；compared with MG63+LV-Vector group， the proliferation activity and number of angiogenesis in the HUVEC in MG63+LV shMIAT group were significantly decreased（P<0.05），while the proliferation activities and number of angiogenesis in the HUVEC in MG63+LV-MIAT group and IL-4+LV Vector group were significantly increased （P<0.05）.The results of in vivo tumorigenesis experiment of the nude mice showed that compared with LV-Vector group， the volume and weight of transplanted tumor of the mice in LV-MIAT group were significantly increased （P<0.05），and the positive expression rates of CD163 and CD31 in tumor tissue were significantly increased （P<0.05）； the volume and mass of transplanted tumor and the positive expression rates of CD163 and CD31 in tumor tissue of the mice in LV-shMIAT group were significantly decreased （P<0.05）. Compared with LV-shMIAT group， the volume and weight of transplanted tumor of the mice in LV-MIAT group were significantly increased （P<0.05）， and the positive expression rates of CD163 and CD31 in tumor tissue were significantly increased （P<0.05）. Conclusion LncRNA MIAT may regulate the progression of OS by promoting the M2 polarization of macrophages and up-regulating the angiogenesis in tumor tissue.

Objective To investigate the effects of ganoderma lucidum ethanol extract （GLEE） on the biological behavior and Janus kinase 1（JAK1）/signal transduction and transcription activator 3 （STAT3） signaling pathway of the cervical cancer cells， and to clarify its possible mechanisms. Methods The human cervical cancer HeLa cells were randomly divided into control group （given complete culture medium） and low， medium and high doses of GLEE groups（given 25， 50， and 100 mg·L^－1 GLEE）. The inhibitory rates of proliferation of the human cervical cancer HeLa cells in various groups were detected by MTT assay； the clone formation rates of the human cervical cancer HeLa cells in various groups were detected by plate cloning experiment； Transwell chamber assay was used to detect the numbers of migration cells and invasion cells in various groups； the expression levels of JAK1 and STAT3 mRNA in the human cervical cancer HeLa cells in various groups were detected by real-time fluorescence quantitative PCR （RT-qPCR） method； the expression levels of JAK1， phophorylated JAK1（p-JAK1），STAT3， phophorylated STAT3（p-STAT3）， suppressor of cytokine signaling 3（SOCS3）， and protein inhibitor of activated STAT1 （PIAS1） proteins in the human cervical cancer HeLa cells in various groups were detected by Western blotting method. Results Compared with control group， the inhibitory rates of proliferation of the cervical cancer HeLa cells in low， medium and high doses of GLEE groups were increased （P<0.05），the clone formation rates the cervical cancer HeLa cells were decreased （P<0.05），the numbers of migration cells were decreased （P<0.05）， the numbers of invasion cells were decreased （P<0.05），the expression levels of JAK1 and STAT3 mRNA in the cervical cancer HeLa cells were decreased （P<0.05）， and the expression levels of SOCS3 and PIAS1 proteins in the cervical cancer HeLa cells were increased（P<0.05）. Compared with low dose of GLEE group， the inhibitory rates of proliferation of the cervical cancer HeLa cells in medium and high doses of GLEE groups were increased （P<0.05）， the clone formation rates of the cervical cancer HeLa cells were decreased （P<0.05）， the numbers of migration cells were decreased （P<0.05），the numbers of invasion cells were decreased （P<0.05）， the expression levels of JAK1 and STAT3 mRNA in the cervical cancer HeLa cells were decreased （P<0.05），and the expression levels of SOCS3 and PIAS1 proteins in the cervical cancer HeLa cells were increased （P<0.05）. Compared with midium dose of GLEE group， the inhibitory rate of proliferation of the cervical cancer HeLa cells in high dose of GLEE group was increased （P<0.05），the clone formation rate of the cervical cancer HeLa cells was decreased （P<0.05），the number of migration cells was decreased （P<0.05），the number of invasive cells was decreased （P<0.05）， the expression levels of JAK1 and STAT3 mRNA in the cervical cancer HeLa cells were decreased （P<0.05），and the expression levels of SOCS3 and PIAS1 proteins in the cervical cancer HeLa cells were increased （P<0.05）. Conclusion GLEE can inhibit the proliferation， cloning， migration and invasion of the cervical cancer HeLa cells to some extent， which may play a role by up-regulating the expression levels of SOCS3 and PIAS1 in the cells， thereby affecting the JAK1/STAT3 signaling pathway.

Methods Ten Wistar Kyoto rats were selected as normal group， and 40 SH rats were randomly divided into model group and low （25 mg·kg^－1·d^－1）， medium （50 mg·kg^－1·d^－1）， and high doses of CR （100 mg·kg^－1·d^－1） groups， and there were 10 rats in each group. The rats in normal group and model group were intraperitoneally injected with the same amount of normal saline. After administration， the blood pressure， blood lipid levels，atherosclerosis index （AI）， 24 h urine 8-iso-prostaglandin F2α （8-iso-PGF2α） renal excretion of the rats in various groups were detected； HE staining was used to observe the morphology of aorta tissue of the rats in various groups；the serum activities of glutathione peroxidase （GSH-Px） and the serum levels of malondialdehyde （MDA）， nitric oxide （NO）， endothelin-1 （ET-1），and thromboxane B2 （TXB2） of the rats in various groups were detected by ELISA method；the expression levels of RhoA/ RhoA/Rho related kinase 1 （ROCK1）， ROCK2 and C-Jun amino acid terminal kinase （JNK） mRNA in aorta tissue of the rats in various groups were detected by real-time fluorescence quantitative PCR （RT-qPCR） method；Western blotting mehod was used to detect the expression levels of ROCK1， ROCK2， JNK，and phophorylated JNK（p-JNK） proteins in aorta tissue of the rats in various groups. Results Compared with normal group， the systolic blood pressure and diastolic blood pressure， 24 h urine 8-iso-PGF2α renal excretion， levels of serum total cholesterol（TC），triglyceride（TG） and low density lipoprotein cholesterol（LDL-c）， AI，levels of MDA， ET-1 and TXB2， expression levels of ROCK1， ROCK2， and JNK mRNA， expression levels of ROCK1， ROCK2， JNK， and p-JNK proteins in aorta tissue of the rats in model group were increased （P<0.05），the serum acitivity of GSH-Px and serum levels of high density lipoprotein cholesterol（HDL-c） and NO were decreased （P<0.05）. In model group， the aortic wall was rough and thickened， and a large number of foam cells and atherosclerotic necrosis substances were distributed. Compared with model group， the systolic blood pressure and diastolic blood pressure，24 h urine 8-iso-PGF2α renal excretion， serum levels of TC， TG，and LDL-c， AI， levels of MDA， ET-1，and TXB2， expression levels of ROCK1， ROCK2 and JNK mRNA，expression levels of ROCK1， ROCK2， JNK， and p-JNK proteins in aorta tissue of the rats in low， medium， and high doses of CR groups were decreased （P<0.05）， and the serum activity of GSH-Px and serum levels of HDL-c and NO were increased （P<0.05）. In low dose of CR group， the roughness and thickening of the aortic wall and the distribution of the foam cells and atherosclerotic necrosis substances in the aorta tissue were decreased to varying degrees.Compared with low dose of CR group， the systolic blood pressure and diastolic blood pressure，24 h urine 8-iso-PGF2α renal excretion， serum levels of TC， TG， and LDL-c， AI， levels of MDA， ET-1， and TXB2，expression levels of ROCK1， ROCK2 and JNK mRNA in aora tissue， expression levels of ROCK1， ROCK2， JNK， and p-JNK proteins in aorta tissue of the rats in medium dose of CR group were decreased （P<0.05）， while the serum activity of GSH-Px and serum levels of HDL-c and NO were increased （P<0.05）. In medium dose of CR group， the roughness and thickening of the aortic wall and the distribution of the foam cells and atherosclerotic necrosis substances in the aorta tissue were reduced. Compared with medium dose of CR group，the systolic blood pressure and diastolic blood pressure， 24 h urine 8-iso-PGF2α renal excretion， serum levels of TC， TG， and LDL-c， AI， levels of MDA， ET-1， and TXB2， expression levels of ROCK1， ROCK2，JNK mRNA in aorta tissue， expression levels of ROCK1， ROCK2， JNK， and p-JNK proteins in aorta tissue of the rats in high dose of CR group were decreased （P<0.05）， while the serum activity of GSH-Px and serum levels of HDL-c and NO were increased （P<0.05）. The atherosclerotic pathomorphology of aorta wall of the rats in high dose of CR group was significantly improved. Conclusion CR can improve ED and As in the SH rats， and its mechanism may be related to inhibiting the activation of ROCK/JNK signaling pathway. Objective To investigate the effect of crocin （CR） on vascular endothelial dysfunction （ED） and atherosclerosis （As） in the rats with spontaneously hypertension （SH），and to clarify its possible mechanism.

Objective： To discuss the effect of Ghrelin combined with LY294002 on neural differentiation of the adipose-derived mesenchymal stem cells （ADSCs）， and to clarify its mechanism. Methods The morphology of ADSCs was observed under inverted phase contrast microscope， and the positive expression rates of surface antibodies of the ADSCs in various groups were identificated with flow cytometry； the fourth-generation ADSCs were divided into control group （given non-intervention）， LY294002 group ［given 40 μmol·L^－1 phosphatidylinositol-3-kinase （PI3K）/protein kinase B （Akt） pathway specific inhibitor LY294002］，Ghrelin group （given 0.1 μmol·L^－1 Ghrelin）， and Ghrelin+LY294002 group （given 40 μmol·L^－1LY294002+0.1 μmol·L^－1 Ghrelin）.Inverted phase contrast microscope was used to observe the morphology of the ADSCs in various groups after the neural differentiation， immunofluorescence staining was used to detect the percentages of neuron specific enolase （NSE）， nestin antibody，and glial fibrillary acidic protein （GFAP） positive cells in the ADSCs in various groups， and Western blotting method was used to detect the expression levels of PI3K/Akt pathway proteins in the ADSCs in various groups，and the ratios of phosphorylated PI3K（p-PI3K）/PI3K and phosphorylated Akt（p-Akt）/Akt were calculated. Results The expressions of CD90 and CD13 of ADSCs surface antibodies were high，and the expressions of CD34，CD45，and CD106 were low.Compared with control group，parts of the cells in LY294002 group changed from long spindle shape to round shape and the protuberance was short；compared with LY294002 group，the transformation speed of the cells in Ghrelin+LY294002 group from long spindle shape to round shape was quick，and the number of protuberance was increased.Compared with control group， the percentages of NSE and Nestin positive cells and the ratios of p-PI3K/PI3K and p-Akt/Akt in the ADSCs in LY294002 group were decreased significantly（P<0.05）， the percentages of NSE and Nestin positive cells and the ratios of p-PI3K/PI3K and p-Akt/Akt in the ADSCs in Ghrelin group were increased （P<0.05）； compared with LY294002 group， the the percentage of NSE and Nestin positive cells and the ratios of p-PI3K/PI3K and p-Akt/Akt in the ADSCs in Ghrelin+LY294002 group were increased （P<0.05）； there were no significant differences in the percentage of GFAP positive cells in the ADSCs among various groups （P>0.05）. Conclusion Ghrelin promotes the neural differentiation of the ADSCs by activating the PI3K/Akt pathway.

Methods The GES-1 cells from normal gastric mucosa，gastric cancer MGC-803 cells，MKN-45 cells，and HGC-27 cells were collected. The hsa_ circ_ 0009735 small interfering RNA and negative control were transfected into the MGC-803 cells， and the gastric cancer MGC-803 cells were divided into si-hsa_ circ_ 0009735 group and negative control group；the control plasmid and hsa_circ_0009735 over-expression plasmid were transfected into the HGC-27 cells， and the gastric cancer HGC-27 cells were divided into OE-hsa_circ_0009735 group and control plasmid group， and the MGC-803 cells transfected with autophagic plasmid pcDNA-eGFP-LC3 were divided into blank group and different concentrations （0.25， 0.50， 1.00 and 2.00 mg·L^－1） of rapamycin groups.The expression levels of hsa _ circ_ 0009735 in the gastric cancer tissue and cells were detected by real-time fluorescence quantitative PCR （RT-qPCR）method；laser confocal microscope was used to observe the morphology of the MGC-803 cells and the number of autophagosomes in the MGC-803 cells in various groups； Transwell chamber experiment was used to detect the number of migration cells in various groups； flow cytometry was used to detect the percentages of the cells at different cell cycles in various groups； Western blotting method was used to detect the expression levels of microtuble-associated protein light chain 3Ⅱ（LC3 Ⅱ），microtuble-associated protein light chain 3Ⅰ（LC3 Ⅰ），E-cadherin， Cyclin D1， Vimentin，and N-cadherin proteins in the cells in various groups. Results The PCR products were sequenced and showed cyclization sites，which was matched with the sequences of circ RNA hsa_circ_0009735. Compared with the sequences of adjacent tissue， the expression level of hsa_ circ_ 0009735 in gastric cancer tissue was increased （P<0.05）； compared with GES-1 cells， the expression level of hsa_ circ_ 0009735 in the MGC-803 cells was increased （P<0.05）.There was no significant change of the morphology of the MGC-803 cells among 0.25 and 0.50 mg·L^－1 rapamycin groups.Compared with blank group，the number of granules in the MGC-803 cells in 1.00 mg·L^－1 rapamycin group was increased and the boundary was unclear； in 2.00 mg·L^－1 rapamycin group， more MGC-803 cells died. The laser confocal microscope observation results showed that the number of autophagosomes in the MGC-803 cells in 1.00 mg·L^－1rapamycin group had no significant difference compared with 2.00 mg·L^－1 rapamycin group （P>0.05）.Compared with blank group， the ratio of LC3 Ⅱ/LC3 Ⅰ in the MGC-803 cells in different concentrations of rapamycin groups were increased （P<0.05）， the expression levels of hsa_circ_0009735 were decreased （P<0.01）. Compared with negative control group， the expression level of hsa_circ_0009735 in the MGC-803 cells in si hsa_ circ_ 0009735 group was decreased（P<0.01）， the ratio of LC3 Ⅱ/LC3 Ⅰ was increased （P<0.01），the number of migration cells was decreased （P<0.01）， the percentage of cells at G₁ phase was increased （P<0.05）， the percentage of cells at S phase was decreased （P<0.01）， the percentage of cells at G₂ phase was decreased （P<0.05）， and the expression levels of Cyclin D1， Vimentin，and N-cadherin proteins in the cells were decreased （P<0.05）； compared with control plasmid group， the expression level of hsa _ circ -0009735 in the HGC-27 cells in OE-hsa_ circ_0009735 group was increased significantly （P<0.01）， the ratio of LC3 Ⅱ/LC3 Ⅰ was decreased （P<0.01）， the number of migration cells was decreased （P<0.01）， the percentage of the cells at G₁ phase was decreased （P<0.01）， the percentage of the cells at S phase was increased （P<0.05）， the percentage of cells at G₂ phase was increased （P<0.05）， the expression level of E-cadherin protein in the cells was decreased （P<0.05）， and the expression levels of Cyclin D1， Vimentin，and N-cadherin proteins in the cells were increased （P<0.05）. Conclusion High expression of hsa_circ_0009735 in the GC cells may promote the EMT process and affect the migration and the cell cycle， and inhibit the autophagy. Objective To investigate the expressions of circular RNA hsa_circ_0009735 in the gastric cancer tissue and cells， and to explore its effects on the epithelial mesenchymal transformation（EMT）， migration， cell cycle，and autophagy of the gastric cancer cells.

Objective： To investigate the clinicopathological features and prognosis-related factors of the patients with solitary fibrous tumor （SFT）， and to provide the evidence for its pathological diagnosis， clinical treatment and prognosis judgment. Methods The clinicopathological data of 86 patients with SFT who had undergone surgical resection from different systems were collected and divided into low （n=65）， medium （n=14） and high risk groups （n=7） according to the risk classification criteria. The general morphology of tumor was observed，HE staining and immunohistochemical staining were used to detect the morphology of SFT，and the follow-up data of the patients were obtained for the prognosis-related correlation analysis. Kaplan-Meier survival curve method was used to analyze the relationship between single clinicopathological factor in different parts and progression free survival of the patients， and Cox regression analysis was used to analyze the relationships between multiple factors and progression free survival of the patients. Results Of the 86 cases of SFT， 37 were male and 49 were female. There was no significant difference in the gender constituent ratio of the patients with SFT at different sites （P>0.05）. There was no significant difference in the age of patients with SFT at different sites （P>0.05）. There was no significant difference in the distribution of SFT at different sites among low，medium， and high risk groups （P>0.05）. There was significant statistical difference in the tumor diameter at different sites（P<0.01）. The microscope results showed that the shape and arrangement of tumor cells were diverse， and the spindle or oval cells were not arranged structurally in varying density； the characteristic antler like branching vessels and collagen fibers of varying thickness were common；most of the tumor cells were mild in shape and heterotypic， and the mitotic image was not obvious.The immunohistochemiscal staining results showed that the STAT-6 nucleus was diffusely and strongly positive； CD34， Bcl-2 and CD99 were positive in different degrees. A total of 61 cases were followed up for 2－139 months. Among them， 10 cases recurred， and the recurrence rates were 9% in low risk group， 14% in medium risk group， and 28% in high risk group， respectively. The univariate analysis results showed that there was a significant difference in the progression free survival between the patients with mitotic images<4/10 HPF and those with mitotic images ≥ 4/10 HPF （P<0.05）； there were significant differences in the progression free survival between high risk group and low，medium risk groups（P<0.05）. The multivariate analysis results showed that gender， age， tumor diameter and mitotic count were not the independent predictors of progression free survival of the patients （P>0.05）. Conclusion SFT can occur in many organs and systems of human body， and its morphology is diverse. The diameters of tumors in the central nervous system， upper respiratory tract and orbit are significantly smaller than those in female genital tract， abdominal cavity， subcutaneous soft tissue， lung and pleura. STAT-6 is a specific and sensitive index for SFT diagnosis； mitotic images ≥ 4/10 HPF and high risk classification are the risk factors for the progression free survival shortening； multivariate comprehensive analysis of tumor risk classification can not fully reflect the prognosis of the patients.

Objective To analyze the effect of Shenqi Dihuang Decoction in the treatment of membranous nephropathy （MN） based on network pharmacology method and molecular docking technique，and to clarify its mechanism. Methods The effective components and corresponding target proteins of 7 herbs of Shenqi Dihuang Decoction were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP）；the target genes of MN were collected from the GeneCards database，OMIM database， and Drugbank database； the network of “components-compounds-targes” of Shenqi Dihuang Decoction was constructed by Cytoscape software； the protein-protein interaction network was constructed through STRING database and Cytoscape software， and the topological analysis was performed to identify the core targets； the molecular docking for the core targets with the active ingredients acting on them was performed to screen the core components of Shenqi Dihuang Decoction for the treatment of MN.Gene Ontology （GO） enrichment analysis and Kyoto Encyclopedia of Gene and Genomes （KEGG） pathway enrichment analysis were performed by Bioconductor R software. Results A total of 55 effective components，214 drug action targets，3 655 MN targets， and 144 intersection targets were obtained，including 5 core targets，which were protein kinase B1 （AKT1）， epidermal growth factor （EGF）， vascular endothelial growth factor A （VEGFA）， tumor protein P53 （TP53）， and epidermal growth factor receptor （EGFR）； three potential core components were screened based on the molecular docking technology， including quercetin， luteolin， and diosgenin；the GO enrichment analysis results revealed 2 268 biological processes， 53 cell compositions，and 175 molecular functions； the KEGG pathway enrichment analysis results revealed 169 related signal pathways. Conclusion Shenqi Dihuang Decoction may play a role in treatment of MN by reducing the level of inflammation， inhibiting the renal fibrosis and maintaining the integrity of glomerular filtration barrier.

Objective To investigate the risk factors for progression of cervical intraepithelial neoplasia Ⅰ （CIN Ⅰ） patients， and to provide more appropriate treatment plans for the CINⅠ patients. Methods The clinical data of 210 patients with CINⅠ confirmed by initial cervical pathological biopsy and conservative treatment were retrospectively analyzed.All the patients were followed up for 24 months to observe the disease progression. Univariate analysis and multivariate Logistic stepwise regression anslysis were used to analyze the influence of patients’ age， human papillomavirus （HPV），liquid based thin layer cytology （TCT）， menopausal status，and transformation zone （TZ）types and image features under colposcope（abnormal vessels，hyperplasia，lesion area， and lesion boundary） on disease progression of the CINⅠ patient at the time of initial diagnosis of CINI disease. Results A total of 40 patients had progressive disease，170 patients had no progressive disease.The univariate analysis results showed that there were statistically significant differences in age （χ²=25.94，P<0.01），TCT stuation （χ²=10.97，P<0.01）， HPV（16/18） infection or not （χ²=4.10，P=0.043）， hyperplasia（χ²=8.18， P=0.004） and menopause status（χ²=25.56， P<0.01）of the pateints between two groups.The multivariate Logistic stepwise regression analysis results showed that age≥45 years old（OR=5.95，P=0.005，95%CI：1.70－20.84），TCT≥ASC-H（OR=3.319，P=0.007，95%CI：1.38－7.97），atypia（OR=9.91，P<0.01，95%CI：2.95－33.34）and hyperplasia （OR=8.51，P<0.01，95%CI：2.60－27.84） were the risk factors for disease progression of the CINⅠ patients. Conclusion Age≥45 years old，TCT≥ASC-H，abnormal blood vessels under colposcope and hyperplasia in the lesion area are the independent risk factors for the disease progression of the CINⅠpatients.

Objective To mine the public databases by using bioinformatics methods and construct the competitive endogenous RNA（ceRNA） immune regulatory network of atrial fibrillation （AF）， and to understand the mechanism of occurrence and development of AF. Methods The gene expression data of circular RNA （circRNA）（GSE129409）， micro RNA （miRNA） （GSE28594） and mRNA（GSE41177） in the AF patients and healthy controls were downloaded from Gene Expression Omnibus（GEO）.The differentially expressed circRNA， miRNA， and mRNA were discovered using the “limma” Data package in R software， and were then visualized by using the pertinent databases. The regulatory relationships between the differentially expressed circRNA， miRNA and mRNA were predicted by ENCORI，circBank，TargetScan and miRDB Databases， and the ceRNA regulatory networks were constructed based on the circRNA-miRNA pairs and the miRNA-mRNA pairs.The DAVID database was used for Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis of differentially expressed mRNA（DEmRNA） to annotate its functions. The best gene characteristics were screened by receiver operating characteristic curve （ROC） curve and the area under curve（AUC） was calculated. CIBERSORT software was used to analyze the infiltration of the immune cells in AF. Results A total of 103 differential circRNAs， 37 differential miRNAs，and 296 mRNAs were identified （|log2（FC）|>1 and P<0.05）， among them 589 miRNAs were predicted to bind to the differentially expressed circRNAs， 9 miRNAs were obtained by intersecting the predicted miRNAs with differentially expressed miRNA（DEmiRNA）， 3 000 target genes of differentially expressed miRNAs were predicted， and 32 differential genes were obtained by intersecting the predicted target genes with differentially expressed genes （DEGs）. Finally，7 circRNAs，5 miRNAs，and 19 mRNAs were assembled into a circRNA-miRNA-mRNA network. The differential genes in the ceRNA network were mainly enriched in the biological processes such as protein degradation， cytosolic spitting action of cells， and protein tyrosine kinase activity.The KEGG enrichment analysis results showed that the DEGs were mainly enriched in the signaling pathways such as chemokine signaling pathway， hedgehog signaling pathway， T lymphocyte receptor signaling pathway and cell-cytokine interaction. The ROC curve results showed that MAL2， STT3B， SHISA3， ZBTB41， CPNE4， EPHA7， hsa_circ_0006562 hsa_circ_0024957， hsa-miR-199a-5p，and hsa-miR-142-3p were identified as the viable candidates for predicting AF （AUC>0.8）. Conclusion The construction of circRNA-miRNA-mRNA network provides a basis for the study of RNA interaction mechanism in AF，and circRNA may be a potential therapeutic target of AF.

Objective：Objective To investigate the genes related to lactic acid metabolism in lung adenocarcinoma （LUAD） tissue and establish the prognostic score model based on the genes related to lactic acid metabolism， and to clarify its ability to predict the prognosis of LUAD. Methods The LUAD related lactate metabolism genes were screened by The Cancer Genome Atlas（TCGA） database.Univariate and multivariate Cox regression analysis and LASSO regression analysis were used to obtain the key genes and the lactate metabolism score model for LUAD was constructed，and the predictive ability of the model was verified by Kaplan-Meier survival analysis and receiver operating characteristic（ROC） curve， and the relationships between the model and the clinical characteristics of the patient and the abundances of immune cell infiltration were evaluated by TIMER method. Results Sixteen lactate metabolism genes were successfully screened and the score model was constructed. The survival analysis results showed that the overall survival（OS） of the patients in low-risk group was significantly higher than that in high-risk group （P<0.01）， and the area under ROC curve （AUC） was higher than 0.7；the multivariate Cox regression analysis results showed that 23 lactic acid metabolism genes were the independent prognosis genes for the LUAD patients，which could accurately assess the survival rate of LUAD patients in combination with other clinical features of the patients（P<0.01），and the lactic acid score was negatively correlated with the percentages of B lymphocytes（r=－0.326，P<0.001），CD4 T lymphocytes（r=－0.196，P<0.001），CD8 T lymphocytes（r=－0.094，P=0.036），macrophages（r=－0.198，P<0.001），and dendritic cells（r=－0.119，P=0.008）. Conclusion The lactate metabolism score model can well evaluate the prognosis of LUAD and the state of tumor microenvironment （TIMER）， and can be used as a biomarker to predict the prognosis of LUAD.

Methods The gastric cancer tissue and the adjacent tissue of 68 patients with gastric cancer confirmed by pathological diagnosis were collected.The expression levels of miR-431-3p mRNA and carboxy-terminal domain phosphatase 1 （CTDP1） mRNA in gastric cancer tissue， adjacent tissue， human normal gastric mucosal epithelial GES-1 cells and human gastric cancer cells （MKN-28， MGC-803， MKN-45， SGC-7901， HGC-27 cells ）were detected by real-time fluorescence quantitative PCR （RT-qPCR） method；the expression levels of CTDP1 protein in the above cells and the expression levels of cytochrome C（Cyt C），B cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） in the SGC-7901 cells in various groups were detected by Western blotting method； the correlation between miR-431-3p and CTDP1 mRNA expression level was analyzed by Pearson correlation analysis；the targeting relationship between miR-431-3p and CTDP1 was detected by dual luciferase reporter gene experiment； the miR-431-3p mimic and CTDP1 over-expression lentivirus were transfected into the SGC-7901 cells separately or at the same time.The cells were divided into blank group， vector over-expression（mimic NC） group，miR-431-3p over-expression （miR-431-3p mimic） group，vector lentivirus（vector） group， CTDP1 over-expression lentivirus （CTDP over-expression）group and miR-431-3p mimic+CTDP1 over-expression （co-transfection） group.The proliferation activities of the cells in various groups were detected by MTT assay； the clone formation numbers of the SGC-7901 cells in various groups were detected by clone formation assay；the apoptotic rates of the SGC-7901 cells in various groups were detected by flow cytometry. Results Compared with the adjacent tissue， the expression level of miR-431-3p in the gastric cancer tissue was decreased （P<0.01），and the expression level of CTDP1 mRNA was increased （P<0.01）， and there was a negative correlation between them （r=－0.316， P=0.009）. Compared with the GES-1 cells， the expression levels of miR-431-3p in the other five kinds of gastric cancer cells were decreased （P<0.01）， and the expression levels of CTDP1 mRNA and protein were increased （P<0.05）. The results of dual luciferase reporter system showed that miR-431-3p targetedly regulated the expression of CTDP1. Compared with blank group and mimic NC group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity，and number of clone formation of the SGC-7901 cells in miR-431-3p group were decreased （P<0.05）， while the apoptotic rate， expression levels of Cyt C and Bax proteins were increased （P<0.05）. Compared with blank group and vector group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity， and number of clone formation of the SGC-7901 cells in CTDP1 over-expression group were increased （P<0.05）， while the apoptotic rate， the expression levels of Cyt C and Bax proteins were decreased （P<0.05）. Compared with blank group and miR-431-3p group， the expression levels of CTDP1 and Bcl-2 proteins， proliferation activity，and number of clone formation of the SGC-7901 cells in co-transfection group were increased （P<0.05）， while the apoptotic rate， expression levels of Cyt C and Bax proteins were decreased （P<0.05）. Conclusion Over-expression of miR-431-3p can inhibit the proliferation and promot the apoptosis of the human gastric cancer cells， and its mechanism may be related to targeted down-regulation of the CTDP1 gene expression. Objective To discuss the effect of miR-431-3p on the proliferation and apoptosis of the gastric cancer cells，and to elucidate its possible molecular mechanism.

Objective To compare the pregnant outcomes of the patients with and without transient premature of luteinizing hormone （LH） in gonadotrophin releasing hormone（GnRH） antagonist flexible protocols who underwent fresh in vitro fertilization / intracytoplasmic sperm injection-embryo transfer （IVF/ICSI-ET） cycles， and to explore whether transient premature had adverse effect on the pregnancy outcomes. Methods The clinical data of 145 IVF/ICSI-ET patients who received flexible antagonist regimen to promote ovulation were analyzed retrospectively. They were divided into non-transient premature of LH group （n=105） and transient premature LH group （n=40）according to whether the patients had the transient premature of LH.The clinical indicators， laboratory indicators and clinical pregnancy outcomes of the patients were compared between two groups. Results There were no significant differences in the average age， infertility years，body mass index（BMI）， basic follicular stimulating hormone（FSH）， basic LH， basic estradiol（E₂）， anti-Mullerian hormone（AMH）， number of basic antral follicles（AFC）， percentages of the patients with poor ovarian reserve and polycystic ovarian syndrome（PCOS） patients between two groups （P>0.05）.The gonadotropin（Gn）days， LH level and E₂ level on the initial day of antagonists of the patients in transient premature of LH group were higher than those in non-transient premature of LH group （t=－3.251， P= 0.001； t=－10.198，P<0.001； t=3.233，P=0.002）， while the days of antagonist use were less than those in non-transient premature of LH group （t=3.090， P=0.003）. There were no significant differences in follicular diameter on the initial day of antagonists， E₂ and progesterone（P）levels and endometrial thickness on the trigger day， number of available oocytes， numbers（rates） of MⅡ oocytes， numbers（rates） of normal fertilization， numbers（rates） of D3 available embryos， numbers（rates） of D3 high-quality embryos， embryo implantation rates， clinical pregnancy rates and ongoing pregnancy rates of the patients between two groups （P>0.05）. The multivariate Logistic regression analysis results showed that the Gn days and E₂ level on the initial day of antagonists were the risk factors for the transient premature of LH of the patients underwent fresh IVF/ICSI-ET cycle antagonist flexible protocol［OR （95%CI）=1.516（1.128－2.038），P=0.006；OR（95%CI）=2.424（1.345－4.371），P=0.003］. Conclusion The transient premature of LH of the patients with antagonist flexible protocol during superovulation does not affect the pregnancy outcomes， but it is necessary to control the increased LH level in time and pay attention to the timing of adding antagonists.

Objective To investigate the clinical effect of extensor indicis proprius-to-extensor pollicis longus transfer in reconstructing the function of thumb drosal extension， and to provide the basis for the treatment of the disease. Methods The preoperative detailed physical examination and color Doppler ultrasound positioning were performed in the patients with spontaneous rupture of the extensor pollicis longus tendon from 2020 to 2021， and the transposition of the extensor indicis proprius was performed. The follow-up data of the patients were collected after the operation. The patients’ bilateral dorsiflexion heights of index fingers，dorsiflexion heights of thumb， thumb opposition distance，active extension angles of index finger metacarpophalangeal （MP） joint and proximal interphalangeal （PIP） joint were measured， and the Special EI-EPL Evaluation Method（SEEM） score was used to evaluate the surgical effect； through the telephone follow-up，the Brief Michigan Hand Questionnaire（BMHQ） score was scored in the form of questionnaire， and Mann-Whitney U test was used to evaluate the preoperative and postoperative overall function， daily living ability， work situation， degree of self-satisfaction， pain and total score of the patients before and after operation，so as to comprehensively evaluate the patients’ hand function， self satisfaction and the effect of extensor polliria tendon of the index finger transfer in reconstruction of function of extensor pollicis longus tendon. Results A total of 8 patients were followed up for 11－24 months， with an average follow-up of（14.8±4.2 ）months. The hands of 8 patients had good appearance without obvious scarring. There was no tendon rupture after the plaster was removed 3 weeks after operation， the tendon tension was appropriate， and the active flexion and extension of the thumb could be successfully completed. The postoperative SEEM score showed that the dorsi-extended height of the index finger on the affected side was 0.4－4.6 cm，with an average of 2.1 cm，which was lower than that on the opposite side；the loss of thumb dorsal extension elevation was 0.4－2.6 cm， with an average of 1.5 cm； the thumb opposition distance was 1.8－3.5 cm， with an average of 2.6 cm； the loss of active extension of the index finger MP joint was 0°－14°， with an average of 3°；the loss of active extension of the index finger PIP joint was 3° to 16°， with an average of 8.5°； the total score ranged from 60 to 90， with an average score of 73.8； two cases were excellent， five cases were good， and one case was normal， with an excellent and good rate of 87.5%. The BMHQ score results showed that there were significant differences in the overall hand function， daily living ability， work situation， patient’s self-satisfaction degree， pain and total score of the patients before and after operation（P<0.01）. Conclusion Extensor indicis proprius tendon has a definite effect in the treatment of spontaneous rupture of the extensor pollicis longus tendon. The thumb extension function is good after operation， and the subjective satisfaction of the patients is high. It is a reliable choice to reconstruct the function of the extensor pollicis longus tendon.

Methods The clinical manifestations of one patient with ITTC were observed.The general morphology of the tumor was observed with naked eyes， laboratory examination and imaging examination were performed， the fine needle aspiration cytology of the thyroid tumor was observed， the morphology of the tumor tissue was observed under light microscope， immunohistochemistry staining and in situ hybridization were performed in the tumor tissue， and the patient was followed up 3 and 11 months after operation；the relevant literatures were reviewed. Results The main clinical manifestation of the ITTC patient was a slightly hard mass in the thyroid.The fine needle aspiration cytology results showed that the tumor cells showed a three-dimensional structure，and the prominent nucleoli could be seen in some cells. The histology results showed that the cancer cells were nest like or island like and differentiated into the squamous cells； the fibrous tissue proliferated accompanying with lymphocyte infiltration around the cancer cells and the right cervical lymph node metastasis. The immunophenotype detection results showed that the cancer cells expressed CD117， CD5 and P63， but didn’t express TdT and TTF-1，EBER-ISH （－）. The pathological diagnosis was ITTC accompanying with cervical lymph node metastasis. No recurrence or metastasis was found on CT 3 and 11 months after operation. Conclusion ITTC has low malignancy degree，which can relapse and metastasize，and combined with surgery and radiotherapy， the prognosis of the patients is good. Objective To study the clinicopathological features， treatment method and prognosis of intrathyroid thymic carcinoma （ITTC），and to strengthen the clinicans’ understandings of the disease.

To analyze the etiology， clinical manifestations and treatment of one patient with type 1 diabetes mellitus（T1DM） complicated with periodontitis，and to clarify the importance of early prevention and treatment of periodontitis associated with T1DM， and to provide the basis for the diagnosis and treatment for the patients and clinicians. Methods The clinical data of a female patient with the history of T1DM for 8 years were collected and the patient visited houspital due to “gingival atrophy for 1 year”. During the treatment， the blood glucose level of patient was monitored for a long time， and the color， morphology and texture of gingiva， probing depth（PD） of periodontal pocket， positive rate of bleeding on probing（BOP），and morphology of the alveolar bone were recorded 6 weeks，3 months，6 months，and 1 year after the initial periodontal therapy. The related literatures were reviewed，and the etiology， clinical characteristics，and the methods of prevention， diagnosis and treatment of the T1DM patient complicated with periodontitis were summarized. Results Before and after initial periodontal therapy， the blood glucose level of the patient was stable without significant fluctuations. Six weeks after initial periodontal therapy， the color of the patients’ gingiva became lighter， the degree of swelling was decreased， the texture was tough， the PD of periodontal pocket was decreased， and the positive rate of BOP was decreased to 13%. One year after initial periodontal therapy， a small amount of tartar and soft dirt could be seen on some tooth surfaces， and the gingiva of the other teeth were light pink， thin and tough.The PD was 0－4 mm， and the BOP percentage was about 14%， and there was no loose teeth in the whole mouth. Conclusion The patients with T1DM complicated with periodontitis should undergo the regular periodontal examinations and strict plaque control. On the premise of stable blood glucose， the initial periodontal therapy can effectively reduce the occurrence and development of the periodontal disease.

Methods The clinical symptoms and signs， imaging findings and postoperative pathological results of one patient with renal SFT complicated with hydronephrosis were collected， the relevant literatures were reviewed，and the clinical characteristics， diagnosis and treatment of the patient with renal SFT complicated with hydronephrosis were summarized. Results A 30-year-old male patient was admitted to the hospital due to a left renal mass with hydronephrosis for 3 d detected by routine physical examination.The abdominal ultrasonography results revealed a left renal mass and left hydronephrosis， with a 2.2 cm separation of light spots in the collecting system； the CT examination results further revealed a left renal space-occupying lesion with hydronephrosis， and the possibility of renal clear cell carcinoma was considered. The preliminary diagnosis was the left renal malignancy， after preoperative preparation， the left transabdominal radical nephrectomy was performed；the postoperative microscope detection results showed that the cell-rich area and the cell-sparse area were alternated， and the spindle cells showed no structural arrangement. Combined with the immunohistochemical staining results ［signal transducer and activator of transcription 6 （STAT6）（+），CD34（+），Vimentin（+），CD99 （partial+）］，the diagnosis of renal SFT was confirmed. The patient recovered well after operation with grade A healing of the incision and was discharged without complications. The CT examination results showed that there was no abnormal manifestation of local recurrence and metastasis 1 month after operation， and no symptoms of discomfort were reported during the follow-up of 3 months after operation. Conclusion Renal SFT has no specific clinical manifestations and can be easily diagnosed as other tumors； the diagnosis mainly depends on the histopathology and immunohistochemical staining results，and currently surgery is still the first option for the treatment of renal SFT. Objective To analyze the clinical manifestations， diagnosis and treatment methods of the patient with renal solitary fibrous tumors （SFT） complicated with hydronephrosis，and to provide the understandings of the clinicians for SFT.

Methods The clinical data of one patient with cardiogenic shock complicated with hypoxemia treated with ECMO and IABP were collected， the etiology and treatment method of hypoxemia were analyzed， and the related literatures were reviewed. Results The patient，48-year-old male， was hospitalized because of paroxysmal precordial pain for 1 d and exacerbation for 4 h. The coronary angiography was performed 1 h after hospitalization，and the proximal anterior descending branch occlusion was found，and the anterior descending branch stent was implanted. The patients’s intraoperative blood pressure was 92/60 mmHg and the heart rate was 110 min^－1， and the IABP （1∶1 counterpulsation） was installed. The heart failure occurred in the patient on the second day after operation，and the patient failed the routine cardiotonic and diuretic treatments and was transferred to Intensive Care Unit （ICU） Department for the ECMO support. On the second day of ECMO support， the patient had a sudden aggravation in the circulation and respiration （the heart rate was raised up to 150－160 min^－1，the blood pressure was dropped to 98/79 mmHg，the respiratory rate was increased to 35 min^－1）， and the percutaneous arterial oxygen saturation （SpO₂） was dropped to 89%. The bedside ultrasound examination results showed that and there was a weak motivation of ventricular of heart and the left ventricle was full.After investigation， it was considered to be caused by membranous pulmonary edema and high frequency of the IABP counterpulsation. The oxygenation state was obviously improved after optimizing the mechanical ventilation setting，the ECMO sweep flow was switched to 10 L·min^－1， and a mass of water droplets were discharged from the membrane exhaust hole；after the IABP counterpulsation frequency was set as 1∶2， the patient’s condition was significantly relieved； finally the ECMO and IABP were successfully removed， and the patient recovered and was discharged from hospital. Conclusion The non-alarm malfunction and improper setting of the extracorporeal equipment can lead to the decreasing of the support performance and aggravation of the disease； when the abnormity occurs， the essential vital signs of the patient should be maintained， then the malfunctions of the equipment should be checked quickly. Objective To analyze the clinical manifestations and treatment method of hypoxemia patients treated with of extracorporeal membrane oxygenation （ECMO） combined with aortic balloon pump （IABP），and to improve the management， identification and analysis abilities of the clinicians for hypoxemia during the treatment of ECMO combined with IABP.

Methods A total of 7 560 healthy physical examination population aged 20－79 years old in the Physical Examination Center of Antu County Hospital in Yanbian Korean Autonomous Prefecture were selected by cluster sampling method. They were divided into MS and its related metabolic abnormality group and MS and its related metabolic index normal group according to the diagnostic criteria of MS. Gender， age， height， body mass， systolic blood pressure， diastolic blood pressure， and fasting serum glucose（FBG）， triglycerides（TG），high-density lipoprotein cholesterol（HDL-c）， SUA and γ- GGT and other indicators the subjects in various groups were collected. Logistic regression analysis was used to detect the risks of different metabolic index disorders of the patients，and to analyze the interactions of different metabolic disorders of the patients. Results The risks of hyperuricemia （HUA） and elevated γ-GGT level of the subjects in MS and its related metabolic abnormality group were higher than those in MS and its related metabolic index normal group， and the risk of MS and its related metabolic abnormalities were increased gradually with the increasing of SUA and γ- GGT levels. The risks of increased HUA and γ-GGT levels of the subjects in MS and its related metabolic index abnormality group were 4.660 times（95%CI：3.571－6.085） and 5.260 times（95%CI：4.009－6.897） higher than those in MS and its related metabolic index normal group； with the increasing of degrees of the metabolic abnormalities， the risks of elevated HUA and γ-GGT levels of the subjects were increased.The risks of obesity， hypertension，dyslipidemia， hyperglycemia and MS of the subjects in increased SUA level and increased γ-GGT group were 1.930（95%CI：1.617－2.304）， 1.618（95%CI：1.352－1.935），4.011（95%CI：3.245－4.958），1.989（95%CI：1.654－2.393）， and 2.695（95%CI：2.248－3.230） times higher than those in normal SUA level and normal γ-GGT level groups，respectively. There was additive interaction between increased SUA and γ-GGT levels on dyslipidemia， and the synergy index（S） and attributable proportion due to interaction were 1.17 and 10.9%， respectively. Conclusion The SUA and γ-GGT levels are closely related to the risks of MS and its related metabolic abnormalities， and the increased SUA and γ-GGT levels are associated with the degree of metabolic abnormalities. HUA combined with increased γ-GGT level can significantly increase the risks of hypertension， dyslipidemia and MS， and increased SUA and γ-GGT levels have an additive interaction on the dyslipidemia. Objective To analyze the relationship between the serum uric acid （SUA） and γ-glutamyl transpeptidase（γ-GGT） levels and metabolic syndrome （MS） and discuss the interaction of SUA and γ-GGT in MS， and to provide the basis for the risk prediction and early intervention of MS.

Objective To construct the shRNA interference vector and over-expression vector of N-myc downstream regulatory gene 1 （NDRG1） and transfect the human cerebral microvascular endothelial cells （hCMECs），and to verify the interference and over-expression effects and to obtain the cells stably over-expressing NDRG1. Methods The pGPU6-GFP plasmid was double digested by BamH Ⅰ and Bbs Ⅰ， and then ligated with eight designed shRNAs to construct the pGPU6-GFP-NDRG1 shRNA interference vectors respectively，and the hCMECs were transfected by Lipofectamine 2000 after sequencing and identification， and the transfection efficiency of the vectors was observed under fluorescence microscope； the sequence of NDRG1 coding region amplified by PCR was ligated with the pMD19-T， after correct sequencing， the recombinant plasmid and the empty plasmid pcDNA3.1 were digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ， and the pcDNA3.1-NDRG1 over-expression vector was constructed after ligation；the hCMECs were transfected with the correctly sequenced over-expression vector by using Lipofectamine 2000；the expression levels of NDRG1 mRNA in the hCMECs were detected by real-time fluorescence quantitative PCR （RT-qPCR） method，and the expression levels of NDRG1 protein in the hCMECs were detected by Western blotting method；the positive clone hCMECs were screened by neomycin. Results After the pGPU6-GFP vector was doubly digested，the electrophoresis results showed that it was completely linear， and the sequencing results showed that the shRNAs were all successfully connected to the pGPU6-GFP vector；the transfection efficiency of vector was about 70 % observed under fluorescence microscope； the RT-qPCR results showed that there were three shRNA interference vectors with good interference effects， and the expression levels of NDRG1 mRNA in the hCMECs after transfection were about 26%， 21%， and 13% of those in control group， respectively；the corresponding specific protein bands were obtained by Western blotting method，the recombinant plasmid pcDNA3.1-NDRG1 was identified by enzymatic digestion， and two DNA bands of 1 185 and 5 428 bp were identified by electrophoresis，and the sequencing results showed that the NDRG1 gene was successfully inserted into the pcDNA3.1 vector， and the positive clone hCMECs were successfully constructed.The RT-qPCR results showed that the expression level of NDRG1 in the positive clone hCMECs was about 25.96 times higher than that in control group； the Western blotting results showed that the expression level of NDRG1 protein in the hCMECs was increased compared with control group. Conclusion The pGPU6-GFP-NDRG1 shRNA interference vector is successfully constructed and the interference effect is verified； the pcDNA3.1-NDRG1 over-expression vector and the hCMECs stably over-expressing NDRG1 are successfully constructed.

Objective To investigate the carbapenem drug resistance of the intensive care unit（ICU） patients infected with Acinetobacter baumannii（AB）， and to clarify the clinical application value of real-time fluorescence quantitative PCR（RT-qPCR） method for the detection of drug resistance genes. Methods A total of 285 sputum specimens from the ICU patients were collected for identification of the drug susceptibility by traditional culture method and detection of the drug resistance genes by RT-qPCR method. Statistical analysis was performed on the detection rate of the carbapenem-resistant Acinetobacter baumannii （CRAB）， the coincidence rate of the drug resistance gene detection and the drug resistance of other antibiotics. Results A total of 151 strains of AB were obtained， the detection rate was 52.98%. The drug resistance rate of AB to carbapenem was 72.20%.There was no significant difference in the coincidence rate of OXA-51 gene detected by RT-qPCR method compared with the traditional culture method（P>0.05）. There was no significant difference in the coincidence rate of CRAB detected by OXA-23 gene compared with the traditional culture method （P>0.05）.The drug resistance rates of 108 OXA-23 positive specimens infected with AB to minocycline，cefoperazone/sulbactam， myxin，and tegacyclin were lower. Conclusion The detection rate of carbapenem resistant drugs of AB identified by sputum culture of the ICU patients is high. Compared with traditional culture method， RT-qPCR method is simpler， faster and has a higher consistent rate of detection.The drug resistance rates of CRAB to minocycline， cefoperazone/sulbactam， myxin and tegacyclin are low.