Abstract:

To observe the effects of Injection of Milkvetch Root on blood lipid metabolism,free radical oxidative damage and renal function of rats,and to discuss the  protective mechanism of injection of Milkvetch Root  on kidney of  type 2 diabetic rats.Methods 80 Wistar rats were randomly divided into control group,model group,captopril group,and injection of Milkvetch Root(IMR) group;there were 20 rats in each group.Except for control group,the rats in the other groups were given high sugar and high fat diet for 8 weeks,and the rats were intraperitoneally injected with low dose of streptozocin(STZ) once to induce type 2 diabetic rat models.The rats in IMR  group were  intraperitoneally injected with Injection of Milkvetch Root (2 mL•kg^-1) since the 17th week after establishment of models.The levels of fasting blood glucose(FBG),serum total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL- c),low density lipoprotein cholesterol (LDL-c),urine trace albumin (Ualb),creatinine(Scr),content of urea nitrogen (BUN), urinary albumin excretion rate (UAER),and creatinine clearance rate (CCr),the content of malondialdehyde (MDA) in kidney tissue,and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) of the rats in various groups were detected after 4 weeks.Results  Compared with control group,the levels of FBG,TC,TG,LDL-c,MDA,UAlb,BUN,SCr and UAER were increased;the  HDL-c level,CCr and the activities of SOD and GSH-Px were decreased(P<0.05 or P<0.01).Compared with model group,the levels of serum  TC,TG and LDL-c and level of MDA in kidney tissue,UAER and serum UALb,BUN,and Scr of the  rats in IMR group were significantly decreased(P<0.05 or P<0.01);the activities of serum SOD  and GSH-Px and  CCr  were increased markedly (P<0.05 or P<0.01);the HDL-c level had no significant change.Conclusion   Injection of Milkvetch Root can correct the lipid metabolic disorders and resist free radical mediated lipid peroxidation in the  type 2 diabetic rats to protect their kidneys.

Key words: Injection of Milkvetch Root, type 2 diabetes, blood glucose, blood lipid, free radicals, renal function