Abstract:

Objective To explore the influence of Akt inhibitor MK2206 in the proliferation and apoptosis of tongue squamous cell carcinoma TCA-8113 cells，and to clarify the possible mechanism.Methods The tongue squamous carcinoma TCA - 8113 cells at the logarithmic phase  were randomly divided into control group and 1,5,25,125,250 nmol/L MK2206 groups.The inhibitory rate of proliferation of TCA-8113 cells was detected with MTT method,and the a
poptotic rate of TCA-8113 cells was determined with flow cytometry(FCM),and the expressions of caspase-9,Bad,GSK-3β,p-Akt and T-Akt proteins
in the  TCA-8113 cells were detected with Western blotting method.Results The IC50 of tongue squamous cell carcinoma TCA - 8113 cells after treated with MK2206 for 12,24,and 36 h were (112.54±1.67),(79.67±2.01),and (33.33±1.98) nmol/L.The FCM results showed that the apoptotic rates of TCA-8113 cells after treated with 1,5,25,125, and 250 nmol/L MK2206 for 12 h  were （14.2±0.74）%,（19.3±0.45）%,（35.1±0.45）%,（39.6±0.48）% and （52.1±0.19）%；there were significant differences compared with  control group(P<0.01).The Western blotting method results showed that  the expressions of p-Akt,Bad and  GSK-3β were decreased with the increasingof dose and time of MK2206; compared with  the  β-actin in  control group,the bands got darken;the expression level of caspase-9 was increased,compared with the β-actin in control group,the bands got darken;the  T-Akt protein expression did not change significantly;compared with the β-actin in control group, the  color of bands had  no significant difference. Conclusion Akt inhibitor MK2206 can inhibit the proliferation of tongue squamou
s cell carcinoma TCA-8113 cells and induce apoptosis.

Key words: tongue squamous cell carcinoma; cell proliferation, apoptosis, PI3K/Akt pathway