Abstract:

Abstract:Objective To investigate the cellular proliferation  level in the rostral migratory stream (RMS) of the adult brain in mice expressed human α-synuclein  (SNCA) A30P mutation and to explore the possible effect of SNCA A30P mutation  on  the neurogenesis in adult brain of mice. Methods 12 mice were divided into C57BL group (normal mice),SNCA-/- group (knocking out SNCA mice), and A30P SNCA-/- group (knocking out  SNCA and knocking in SNCA  A30P mice).The  mouse brains were taken out and the coronal sections were made.The  morphologies of RMS of the mice in various groups were  observed;after the free-floating fluorescent immunohistochemistry of phosphohistone 3(PH3),doublecortin(Dcx), and 4′,6-diamidino-2-phenylindole dihydrochloride(DAPI) were carried out,the number of PH3 positive cells and the fluorescence intensity of Dcx-positive neuroblasts were analyzed.Results There were no signifrcant differences of the morphology,size and cell density of mouse RMS between various groups.Compared with normal control group,the number of PH3 positive  proliferating neurons in RMS of mice in  SNCA-/- and A30P SNCA-/- groups were decreased(P<0.05);compared with normal  control group,the fluorescence intensities of Dcx-positive  neuroblasts in the other two  groups were increased slighthy.Conclusion Human SNCA A30P point mutation may  decrease the neuron proliferation,but slightly enhance its migration in RMS of adult brain of mice,which suggests that the mutation has a certain inhibitory  effect on the neurogenesis in  RMS of adult brain.

Key words: &alpha, -突触核蛋白；吻端迁移流；神经发生；大脑