Abstract:

Abstract:Objective To identify the normal breast tissue and breast fibroadenoma  tissue by shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS),
and to explore the biological characteristics of FD and the identification method by discussing its spectroscope characteristics.
Methods The frozen section of 26 patients (all female,aged 19-59 years) were obtained by routine surgical resection.9 cases of normal tissue and 17 cases of breast fibroadenoma tissue were detected by Raman spectroscopy and then SHINERS technique was utilized.A total of 243 Raman and 273 SHINERS spectra were ob
tained.All the spectra were dealt with baseline corrected by fitting and subtracting a third-order polynomial and then smoothed with a 15-point Adjacent-Averaging.
Results The characteristic peaks of normal breast tissue appeared in 1 090,1 157,1 262,1 300,1 442,1 658,1 745,and 1 874 cm^-1.After adding SHINs,
some peaks shifted in 2-3 cm-1,the relative strengths of 1 090 and 1 157 cm^-1  were significantly increased,and the 1 496 cm-1 characteristic peak appeared.The main characteristic peaks of breast fibroadenoma  appeared in 751,880,930,880,1 262,1 442,1 579,1 658,and 1 745 cm^-1;one of the dominant characteristic peak should belong to lipids,but it can be seen that amide Ⅰ characteristic peak of protein became more significant.Conclusion Raman spectra can discover the differences of the characteristic peaks of amide Ⅰ between breast fibroadenoma  and normal breast tissues.By virtue of different enhancement effects of SHINs to Raman specific peaks of the various tissues,breast fibroadenoma can be distinguished from normal tissue successfully.
 

Key words:  , breast neoplasms, Raman spectroscopy, surface enhanced Raman spectroscopy, fibroadenoma