Abstract:

Objective To select the best extraction solvent of the raw polysaccharide in northeast water chestnut,and to compare the purify effects of 4 kinds of purification methods for raw polysaccharide.Methods The raw extracts from northeast water chestnut were divided into three parts. The raw polysaccharide in northeast water chestnut was extracted with methanol,ethanol,and acetone.The polysaccharide of water-chestnut was deproteinized by Sevag,trichloroacetic acid,hydrochloric,and activated carbon methods. The levels of polysaccharide and protein were detected by colorimetric method,and their purification effects were compared.Results The highest level of the polysaccharide in the water-chestnut extracts was obtained by using acetone as the extraction solvent; the level of polysaccharide was 33.69% and the level of protein was 5.51%.After treated by Sevag,trichloroacetic acid,hydrochloric,and activated carbon,the levels of polysaccharides were 46.53%,43.44%,41.24%,and 36.66%;the levels of proteins were 5.64%,5.41%,5.41%,and 5.51%,respectively.Conclusion Acetone is the best extraction solvent of the raw polysaccharide of northeast water chestnut.The four purification methods have advantages and disadvantages,and the highest polysaccharide level is obtained by Sevag method,and it is suitable for extraction of raw polysaccharide in northeast water chestnut.

Key words: northeast water-chestnut, extract, purification, polysaccharide, protein