过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用

doi:10.13481/j.1671-587x.20150301

Abstract

Abstract:

Objective To establish a superoxiaized model in vitro by using H₂O₂ in Hela cells and to observe the effect of H₂O₂ on the autophagy and apoptosis in Hela cells, and to investigate the relationship between H₂O₂ and endoplasmic reticulum stress(ERS) signal pathway. Methods 1 mmol·L^-1 H₂O₂ was employed to establish in vitro oxidative stress model.The experiment included control group, H₂O₂ 4 h group, H₂O₂ 8 h group, and H₂O₂ 12 h group;the Hela cells in various groups were treated with H₂O₂ for 0, 4, 8, and 12 h, respectively.The expression levels of apoptotic and autophagic proteins in the Hela cells in various groups were detected by Western blotting method. Results Compared with control group, the expression levels of Cleaved Caspase-3 in H₂O₂ groups were significantly increased (P<0.05), and the expression levels of Cleaved Caspase-4 were increased also(P<0.05).The expression levels of LC3-Ⅱ in H₂O₂ groups were also increased significantly compared with control group(P<0.05).The expression levels of GRP78 in H₂O₂ groups were increased compared with control group(P<0.05), and the expression levels of IRE1a(P<0.01) and p-JNK(P<0.05) were higher than those in control group. Conclusion H₂O₂ can induce the autophagy and apoptosis in Hela cells of in vitro oxidative stress model and its possible mechanism is related to ERS signal pathway.

Key words: hydrogen peroxide, oxidative stress, endoplasmic reticulum stress, apoptosis, autophagy, Hela cells

CLC Number:

R737.3

YU Yang, XU Ye, LIU Shibing, YI Dan, LI Songyan, WANG Xiaojun. Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stresssignal pathway[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 437-441.

References

[1] Arbabian A, Brouland JP, Gélébart P, et al.Endoplasmic reticulum calcium pumps and cancer[J].Biofactors, 2011, 37(3):139-149.

[2] Ron D, Walter P.Signal integration in the endoplasmic reticulum unfolded protein response[J].Nat Rev Mol Cell Biol, 2007, 8(7):519-529.

[3] Minamino T, Komuro I, Kitakaze M.Endoplasmic reticulum stress as a therapeutic target in cardiovasculardisease[J].Circ Res, 2010, 107(9):1071-1082.

[4] Hetz C.The unfolded protein response:Controlling cell fate decisions under ER stress and beyond[J].Nat Rev Mol Cell Biol, 2012, 13(2):89-102.

[5] Passmore LA, Booth CR, Venien-Bryan C, et al.Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation[J].Mol Cells, 2005, 20(6):855-866.

[6] Rubinsztein DC.The roles of intracellular protein-degradation pathways in neurodegeneration [J].Nature, 2006, 443(7113):780-786.

[7] Karim MR, Kawanago H, Kadowaki M.A quick signal of starvation induced autophagy: Transcription versus post-translational modification of LC3[J].Anal Biochem, 2014, 22(465C): 28-34.

[8] Muhammad M, Jason R.Mechanism of pyrethroid pesticide-induced apoptosis: role of calpain and the ER stress pathway[J].Toxicol Sci, 2011, 122(2):512-525.

[9] Cadena SG, Hernández-Fonseca K, Camacho-Arroyo I, et al.Glucose deprivation induces reticulum stress by the PERK pathway and caspase-7 and calpain-mediated caspase-12 activation[J].Apoptosis, 2014, 19(3):414-427.

[10] Liu LQ, Fan ZQ, Tang YF, et al.The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro[J].Alcohol Clin Exp Res, 2014, 38(3):683-693.

[11] Walter P, Ron D.The unfolded protein response:From stress pathway to homeostatic regulation[J].Science, 2011, 334(6059):1081-1086.

[12] Ogata M, Hino S, Saito A, et al.Autophagy is activated for cell survival after endoplasmic reticulum stress[J].Mol Cell Biol, 2006, 26(24): 9220-9231.

[13] Shen C, Yan J, Erkocak OF, et al.Nitric oxide inhibits autophagy via suppression of JNK in meniscal cells[J].Rheumatology (Oxford), 2014, 53(6):1022-1033.

[14] Tuñón MJ, San-Miguel B, Crespo I, et al.Melatonin treatment reduces endoplasmic reticulum stress and modulates the unfolded protein response in rabbits with lethal fulminant hepatitis of viral origin[J].J Pineal Res, 2013, 55(3):221-228.

[1]	ZHU Deqiang, CHEN Xuejun. Effects of plumbagin on proliferation and apoptosis of hepatocellular carcinoma HepG2R cells resistant to sorafenib and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1223-1229.
[2]	PAN Hong, WANG Yufeng, HUANG Haipeng, ZHANG Liying, ZHONG Zhen, MA Shiqi, DONG Li, WANG Hongfeng. Influence of Adjust Zang Dredge Meridian electroacupuncture in expression of GRP78 in sciatic nerve cells of rats with diabetic peripheral neuropathy and its neuroprotective effect [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1124-1130.
[3]	GONG Qing, SONG Qiuying, QIU Lijing, HUANG Xiaowei, ZHAO Wenhai. Recovery effect of PLGA microsphere-loaded VAP combined with BMMSCs on sciatic nerve injury of rats and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1174-1178.
[4]	YU Lei, TANG Geng, FANG Fang, LI Xin, GONG Shouliang, WANG Zhicheng, WANG Jianfeng. Expressions of IRE1α and XBP1 mRNA and proteins in mouse testis cells induced by low dose ionizing radiation [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 919-923.
[5]	YAN Hui, MA Shufei, DUAN Minghua, YAN Yuli, PAN Xin, LI Haiqing, ZHOU Changlong, ZHAO Tianyi. Influence of genipin in abilities of proliferation and invasion and apoptosis of gastric cancer SGC 7901 cells and their mechanisms in vitro [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 924-928.
[6]	XING Shugang, ZHANG Lihong, JIN Minghua, LI Baihui, WU Zexin, FANG Bo, LI Wei. Effects of myricetin on proliferation and apoptosis of glioma GL261 cells in mice [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 955-961.
[7]	CHEN Xue, SHEN Nan, AN Ying, XU Bo, ZHAO Lijing. Protective effect of salvianolic acid B on cardiomyocytes of suckling rats with oxidative stress injuryand its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 974-978.
[8]	RAN Qin, ZHANG Lei, ZHANG Yun, QIU Yuhuan, YUAN Xiefang, WANG Xiaoyun, TANG Hongmei, WANG Xing, LI Guoping. Inhibitory effect of resveratrol on pulmonary inflammation in mice with neutrophilic asthma and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 897-902.
[9]	YU Yang, XU Lu, LIU Shibing, LI Songyan, XU Ye. Induction effect of myricetin on apoptosis of human ovarian cancer SKOV3 cells by promoting DRP1-dependent mitochondrial fission [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 903-907.
[10]	YANG Wanxia, SU Qiang, XING Aihua, ZHANG Xiaoyan. Effects of starvation on autophagy and apoptosis of Beclin-1-dependent Ana-1 cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 704-708.
[11]	ZHU Xiaohui, MENG Qin, FENG Shiyan. Expression of TP53 in villus tissue of unexplained recurrent spontaneous abortion and its effect on growth of human trophoblast cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 796-800.
[12]	HUANG Kaiwen, LI Arong, DENG Zhijun, LIU Ruoxuan, LI Changqing, ZHONG Shishun, GUO Jiewen, ZHANG Jie, CHEN Bin. Improvement effect of saponin of litchi seed on oxidative stress of pancreas tissue in impaired glucose regulation rats and its mechanism [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 746-752.
[13]	WANG Miao, MU Runhong, LI Mingcheng, LI Hong Jie. Effects of silencing survivin and HIF-1α genes with RNAi on proliferation and apoptosis of human gastric cancer BGC-823 cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 753-758.
[14]	XIE Wenjie, LONG Haiquan, SONG Daifu, OU Dejin, JIANG Lan, CHEN Ximing, HUANG Jionghua. Level of autophagy-related protein-9a in plasma of hypertension patients with left ventricular hypertrophy and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 786-790.
[15]	AN Le, YANG Jianzheng, HU Chunmei, YU Qiong, XIAO Han, HAO Shuhong, TANG Yan. Anti-apoptosis effect of polycystin-1 gene on human cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 776-779.

Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stresssignal pathway

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0