联麦氧钒对糖尿病大鼠内质网应激介导心肌细胞凋亡的抑制作用及其机制

doi:10.13481/j.1671-587x.20150317

Abstract

Abstract:

Objective To investigate the inhibitroy effect of bis(maltolato)oxovanadium(Ⅳ) (BMOV) on endoplasmic reticulum stress(ERS)-induced apoptosis of cardiomyocytes of the rats with diabetes mellitus (DM) and possible mechanisms, and to provide a theoretical basis for the clinical study on diabetic cardiomyopathy(DCM). Methods 35 male Wistar rats were selected, among them 10 rats were randomly selected and used as control group, and they were admistrated intraperitoneally with citric acid buffer (55 mg·kg^-1) and normal drinking water for 4 weeks.The remaining 25 rats were admistrated intraperitoneally with streptozotocin (STZ).1 week later, the rats with the level of fasting blood glucose greater than or equal to 13.3 mmol·L^-1 were used as DM models. Finally 20 DM rats were randomly divided into DM group and BMOV group (n=10).The rats in DM group were administered with normal drinking water for 3 weeks.The rats in BMOV group were administered with BMOV in drinking water for 3 weeks.The myocardium tissue was taken for TUNEL staining to observe the apoptosis;Western blotting method was used to detect the levels of glucose-regulated protein 78 (GRP78), CCCAAT/enhancer binding homologous protein (CHOP), X-box binding protein 1(XBP-1);qPCR was used to dectect the XBP-1 mRNA expression levels in myocardium tissue of the rats in various groups. Results The TUNEL staining Results showed that the apoptotic cells in cyocardium tissue of the rats in control group were few, in control group the apoptotic index(AI) was 0.80 + 0.63;compared with control group, the apoptosis of myocardial cells in DM group was significantly increased, and the AI was increased (P<0.05);compared with DM group, the apoptosis of myocardial cells in BMOV group was significantly decreased, and the AI was decreased (P<0.05).The Western blotting Results showed that the expression levels of GRP78, CHOP.and XBP-1 of the rats in DM group were significantly higher than those in control group(P<0.05);and the expression levels of GRP78, CHOP and XBP-1 of the rats in BMOV group were lower than those in DM group(P<0.05).The qPCR Results showed that the XBP-1 mRNA expression level of the rats in DM group was higher than that in control group(P<0.05), and the XBP-1 mRNA expression level of the rats in BMOV group was lower than that in DM group(P<0.05). Conclusion BMOV has protective effects on the myocardial cells of the DM rats, and its mechanism may be related to inhibition of ERS and apoptosis of myocardial cells induced by ERS.

Key words: bis(maltolato)oxovanadium(Ⅳ), diabetic cardiomyopathy, endoplasmic reticulum stress, X-box binding protein 1

CLC Number:

R587.2

LI Ying, CONG Xiaoqiang, ZHAO Liangchen, XIE Lin, LIU Ya. Inhibitory effect of BMOV on endoplasmic reticulum stress-induced cardiomyocyte apoptosis of diabetic rats and its mechanism[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 522-526.

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