Abstract:

Objective To explore the association between HLA-DRB1 gene rs2308765, rs9269186 and rs3135388 polymorphisms and essential hypertension(EH), and to clarify the association between single nucleotide polymorphisms(SNPs) of HLA-DRB1 gene and EH. Methods Using case-control study design, 161 patients with EH were recruited in case group and 147 healthy persons were selected as control group.The related factors of EH were collected and the polymorphisms of HLA-DRB1 rs2308765, rs9269186 and rs3135388 sites were detected with PCR-LDR. Results The body mass index(BMI), waist circuit(WC), blood glucose(BG), total cholesterol(TC), triglyceride(TG) and low density lipoprotein cholsterol (LDL-C) levels of the patents in case group were significantly higher than those in control group(P<0.05 or P<0.01), while the level of high density lipoprotein cholesterol (HDL-C) was significantly lower than that in control group(P<0.01).After the potential confounders in the model of Logistic regress were adjusted, the high BMI (OR=1.140, 95%CI:1.040-1.249)and TG (OR=1.215, 95%CI:1.016-1.452) were detected as the independent risk factors of EH.The frequency of the genotype GG and the frequency of G allele of rs2308765 site in case group were significantly lower than those in control group(χ²=4.666, P=0.031;χ²=4.485, P=0.034).The distribution of genotypic and allelic frequencies of rs3135388 and rs9269186 sites were not significantly different between case group and control group(P>0.05).Among the four environmental factors, such as BMI, TG, TC and HDL-C, only rs2308765 polymorphisms had interaction with TG(P<0.05). Conclusion The BMI, TG and SNPs of rs2308765 site of HLA-DRB1 gene are associated with EH.High BMI and high TG are the risk factors of EH, and rs2308765 G/T mutation might be the risk factor of EH.

Key words: essential hypertension, HLA-DRB1 gene, single nucleotide polymorphisms, triglyceride, body mass index