Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (06): 1130-1133.doi: 10.13481/j.1671-587x.20150606

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Expression of dopamine D1 receptors in hippocampal dentate gyrus in rats with vascular dementia

WAN Peng1, GAO Juntao1, WANG Dan2, WANG Shi2, JIN Qinghua2   

  1. 1. Department of Physiology, Jilin Medical University, Jilin 132013, China;
    2. Department of Physiology and Pathophysiology, College of Medical Sciences, Yanbian University, Yanji 133002, China
  • Received:2015-03-17 Published:2016-01-11

Abstract:

Objective To observe the extracellular content of dopamine (DA) and expression of D1 receptors in hippocampal dentage gyrus (DG) in the model rats with vascular dementia (VD),and to investigate the relationship between them. Methods 12 male SD rats were randomly divided VD group and sham-operation group,and the VD model was prepared by permanent bilateral carotid occlusion.The extracellular content of DA in the DG was determined by in vivo microdialysis and HPLC,and the expression of D1 receptors was measured by immunehisto-chemistry. Results The DA content in the DG of the rats in VD group was lower than that in sham-operation group (P<0.05).The number of D1 receptor-positive cells in the DG hilus in VD group was increased compared with sham-operation group (P<0.05),whereas the expression of D1 receptor in DG granule cell layer did not change (P>0.05). Conclusion The DA content in the hippocampal DG is decreased in the VD rats,and its function may be compensated by the up-regulation of D1 receptors in the DG hilus.

Key words: vascular dementia, dentate gyrus, receptors,dopamine D1, rats,Sprague-Dawley

CLC Number: 

  • R743