Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (01): 15-18.doi: 10.13481/j.1671-587x.20160104

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Iihibition of autophagy of prostate cancer PC-3 cells by CD147 with Beclin-1

FANG Fang1, FANG Qing1, ZHAO Liang-zhong1, LI Qiang1, CHEN Shuang1, ZHANG Duo1, WANG Liguo2   

  1. 1. Department of Immunology, School of Medical Laboratory, Jilin Medical College, Jilin 132013, China;
    2. Department of Urinary Surgery, Affiliated Hospital, Jilin Medical College, Jilin 132013, China
  • Received:2015-03-11 Published:2016-01-26

Abstract:

Objective: To establish starvation-induced autophagy model and explore the influence of CD147 in autophagy of prostate cancer PC-3 cells,and to clarify its mechanism.Methods: Starvation method was employed to establish autophagy model. The experiment included negative control group and CD147 shRNA group (PC-3/shCD147). The autophagy spot formation was detected by GFP-LC-3 plasmid transfection,and the LC3-Ⅱ and Beclin-1 protein expressions were determined by Western blotting method. Tryan blue exclusion assay was used to analyze the cell death. Results: Compared with negative control group,the number of GFP-LC3 spot cells in PC-3/shCD147 group was increased (P<0.01),and the expression levels of LC3-Ⅱ and Beclin-1 were also increased (P<0.05 or P<0.01). Meanwhile,the cell death rate in PC-3/shCD147 group (38.4%±3.1%)was increased compared with negative control group (22.3%±3.5%)(P<0.05).Conclusion: CD147 can inhibit the starvation-induced autophagy with Beclin-1,and decrease the autophagy death in prostate cancer PC-3 cells.

Key words: CD147, autophagy, Beclin-1, cell proliferation, PC-3 cells, prostate neoplasms

CLC Number: 

  • R737.25