宫颈鳞状细胞癌组织中miRNA差异性表达及其靶基因作为诊断标志物的意义

doi:10.13481/j.1671-587x.20160117

Abstract

Abstract:

Objective: To study the differential expression of miRNA in cervical squamous cell carcinoma(CESC)tissue and predict its target genes using bioinformatics methods,and to find the miRNA as tumor markers in the pathological process of CESC.Methods: The CESC samples and their matching normal CESC tissue in TCGA database were download,and the differential expression of genes and miRNAs were screened with statistical method,and the target gene prediction was performed for the differently expressed miRNA with target gene prediction websites.KEGG database was used to analyze the target genes involved in tumor related signaling pathway.Results: Compared with their matching normal CESC tissue,21 miRNA and 1 180 genes were found to be differentially expressed(P<0.05),18 miRNA and 411 genes were up-regulated,and 3 miRNA and 770 genes were down-regulated.7 miRNAs had negative correlationship with the predicted target genes;6 up-regulated miRNA were accompanied by their target genes down-regulation,and down-regulated 1 miRNA were accompanied by their target genes up-regulation;their target genes were involved in Wnt,MAPK,P53 and cAMP tumor related signaling pathways.Conclusion: The differentially expressed miRNAs including hsa-miR-27a,hsa-miR-148b,hsa-miR-185,hsa-miR-200a,hsa-miR-200b,hsa-miR-221 and hsa-mir-133b and their target genes play important roles in the development of CESC,and they are possible to become the tumor markers in the diagnosis of CESC.

Key words: cervical squamous cell carcinoma, microRNA, tumor marker

CLC Number:

R737.33

YU Feifei, TIAN Yuxi, LIU Xiaodong, MA Shumei. Differential expression of miRNA in cervical squamous cell carcinoma tissue and significance of its target genes as diagnostic markers[J].Journal of Jilin University Medicine Edition, 2016, 42(01): 85-88.

References

[1] Grieb BC,Chen X,Eischen CM. MTBP is overexpressed in triple-negative breast cancer and contributes to its growth and survival[J].Mol Cancer Res,2014,12(9):1216-1224.

[2] Yang D,Sun Y,Hu L,et al.Integrated analyses identify a master microRNA regulatory network for the mesenchymal subtype in serous ovarian cancer[J].Cancer Cell,2013,23(2):186-199.

[3] Calin GA,Croce CM.MicroRNA-cancer connection:the beginning of a new tale[J].Cancer Res,2006,66(15):7390-7394.

[4] Michael MZ,O'Connor SM,van Holst Pellekaan NG,et al. Reduced accumulation of specific microRNAs in colorectal neoplasia[J].Mol Cancer Res,2003,1(12):882-891.

[5] Wilting SM,Snijders PJ,Verlaat W,et al.Altered microRNA expression associated with chromosomal changes contributes to cervical carcinogenesis[J].Oncogene,2013,32(1):106-116.

[6] Rao Q,Shen Q,Zhou H,et al.Aberrant microRNA expression in human cervical carcinomas[J].Med Oncol,2012,29(2):1242-1248.

[7] Sun J,Ji J,Huo G,et al.miR-182 induces cervical cancer cell apoptosis through inhibiting the expression of DNMT3a[J].Int J Clin Exp Pathol,2015,8(5):4755-4763.

[8] Han Y,Xu GX,Lu H,et al.Dysregulation of miRNA-21 and their potential as biomarkers for the diagnosis of cervical cancer[J].Int J Clin Exp Pathol,2015,8(6):7131-7139.

[9] Song X,Shi B,Huang K,et al.miR-133a inhibits cervical cancer growth by targeting EGFR[J].Oncol Rep,2015,34(3):1573-1580.

[10] 丰有吉,沈铿.妇产科学[M].2版.北京: 人民卫生出版社,2010.

[11] Saavedra KP,Brebi PM,Roa JC.Epigenetic alterations in preneoplastic and neoplastic lesions of the cervix[J].Clin Epigenetics,2012,4(1):13.

[12] Shi TY,Chen XJ,Zhu ML,et al.A pri-miR-218 variant and risk of cervical carcinoma in Chinese women[J].BMC Cancer,2013,13:19.

[13] Wilting SM,Verlaat W,Jaspers A,et al.Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis[J].Epigenetics,2013,8(2):220-228.

[14] Li Y,Liu J,Yuan C,et al.High-risk human papillomavirus reduces the expression of microRNA-218 in women with cervical intraepithelial neoplasia[J].J Int Med Res,2010,38(5):1730-1736.

[15] 莫祥兰,韦荣干,卢军营,等.Sirt1和ProEx C在宫颈高度鳞状上皮内病变细胞学诊断中的价值[J].中国实验诊断学,2015,19(11):1886-1889.

Differential expression of miRNA in cervical squamous cell carcinoma tissue and significance of its target genes as diagnostic markers

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