Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 681-684.doi: 10.13481/j.1671-587x.20160409

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Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance

LIU Yin1,2, SHEN Yuqin1, GAO Han1,2, FEI Hongbo1,2, HU Tianqi1,2, LI Yangyang1,2, GU Zhongyi1,2, LIN Chongtao1   

  1. 1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China;
    2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun 130021, China
  • Received:2015-12-25 Published:2016-07-20

Abstract:

Objective: To investigate the effect of MT01 on the differentiation of osteoblasts under infected condition through determining the expression level of collagen Ⅰ(ColⅠ) mRNA in MG63 cells treated with Porphyromonas gingivalis(Pg). Methods: The cultured MG63 cells were divided into blank control,MT01,Pg, and MT01+Pg groups.MT01 at a concentration of 1 mg·L-1 was added into the MG63 cells,and the cells were incubated for 3 h.The cells treated with PBS (1 mg·L-1) were used as control group.Then Pg(MOI=100∶1)was added.Real-time PCR was used to detect the expression levels of ColⅠ mRNA in MG63 cells at 2,4,6,8,12 and 24 h after incubation. Results: Compared with blank control group,the levles of ColⅠ mRNA in the MG63 cells in MT01 and MT01+Pg groups had no significant changes at 2 and 4 h(P > 0.05);the ColⅠ mRNA expression levels in MT01 group at 8,12 and 24 h were increased(P < 0.05 or P < 0.01).Compared with Pg group, the expression levels of ColⅠ mRNA in MT01+ Pg at 2 and 4 h were decreased,but the expression levels of ColⅠ mRNA were increased at 6,8,12 and 24 h(P < 0.05 or P < 0.01). Conclusion: MT01 can up-regulate the expression level of ColⅠ mRNA in the infected MG63 cells;MT01 could promot the differentiation of osteoblasts under infected condition.

Key words: specific sequence oligodeoxynucleotide MT01, Porphyromonas gingivalis, human osteoblast cell, collagen Ⅰ

CLC Number: 

  • Q254