γδT细胞对卵巢癌SKOV3细胞增殖的抑制作用

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Inhibitory effect of γδT cells on proliferation of ovarian cancer SKOV3 cells

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doi:10.13481/j.1671-587x.20160511

Abstract:

Objective: To study the inhibitory effect of γδT cells on the proliferation of ovarian cancer SKOV3 cells, and to clarify its possible mechanism of inducing apoptosis. Methods: The human ovarian cancer SKOV3 cells cultured in vitro were used as control group, and the γδT and SKOV3 cells were co-cultured for 72 h as γδT cells treatment group.Laser scanning confocal microscope was used to obeserve the morphological changes of nucleus SKOV3 cells,and the inhibitory rate of proliferation of SKOV3 cells in two groups were detected by MTT method;Transwell Chambers was used to detect the cell migration ability,then the apoptotic rates of SKOV3 cells were tested by flow cytometry(FCM). Results: The apoptotic morphology of nucleus of SKOV3 cells in γδT cells treatment group were found under microscope, such as nuclear shrinkage.The MTT resultes displayed that the inhibitory rate of proliferation of SKOV3 cells in γδT cells treatment group was higher than that in control group (P<0.05).The Transwell Chambers results showed that the number of transmembrane cells in γδT cells treatment group was lower than that in control group, and the migration rate was decreased compared with control group (P<0.05).The FCM results showed that the apoptotic rate of SKOV3 cells in γδT cells treatment group was higher than that in control group(P<0.05). Conclusion: γδT cells can inhibit the proliferation and the migration abilities of ovarian cancer SKOV3 cells,and promote the apoptosis.

Key words: γδT cells, SKOV3 cells, ovarian neoplasms, cell proliferation

CLC Number:

R737.31

WANG Haijiao, FAN Yanyan, ZHANG Shi, ZHANG Ruili, ZHANG Haijing, HAN Yahui, ZHU Jihong. Inhibitory effect of γδT cells on proliferation of ovarian cancer SKOV3 cells[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 897-900.

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