新型多级孔生物玻璃对人成骨细胞增殖和分化的促进作用

doi:10.13481/j.1671-587x.20160602

Abstract

Abstract:

Objective: To investigate the effect of a new type of mesoporous bioactive glass with a hierarchical pore structure on the proliferation and differentiation of osteoblasts, and to clarify its osteogenetic differentiation effect. Methods: The MBG structure was observed by scanning electron microscope(SEM);the MG63 cells at logarithmic growth phase were selected and divided into MBG group(cultured with MBG), NBG group(cultured with NBG) and blank control group(cultured with blank cultrue medium), and they were cultured for 1,3,and 5 d.The proliferation rate of MG63 cells was analyzed by MTT method; enzyme-linked immunoassay method was used to analyze the alkaline phosphates(ALP) activity in MG63 cells; real-time PCR was used to detect the relative expression levels of Sp7 mRNA and Runx2 mRNA in MG63 cells after cultured for 24 h. Results: The SEM results showed that the MBG had porous scaffold structure.Compared with blank control group and NBG group,the proliferation rates of MG63 cells in MBG group at 1,3,and 5 d were significantly increased(P<0.01).Compared with blank control group and NBG group, the activities of ALP in the MG63 cells in MBG group at 1,3,5 d were significantly increased(P<0.01). Compared with blank control group and NBG group,the relative expression level of Sp7 mRNA in the MG63 cells in MBG group was significantly increased 24 h after culture(P<0.01), while the relative expression level of Runx2 mRNA in the MG63 cells in MBG group had no significant differences (P>0.05).Conclusion: Compared with NBG,the MBG presents the promotive effect on the proliferation and differentiation of osteoblasts in vitro with low cytotoxicity.

Key words: bioactive glass, osteoblasts, alkaline phosphatase, cell proliferation, differentiation

CLC Number:

R318.5

QI Jia, SUN Xinhua, HUANG Can, LOU Yixin, WANG Yang, SHEN Yuqin. Promotive effect of a new type of mesoporous bioactive glass on proliferation and differentiation of human osteoblasts[J].Journal of Jilin University Medicine Edition, 2016, 42(06): 1049-1053.

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Promotive effect of a new type of mesoporous bioactive glass on proliferation and differentiation of human osteoblasts

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