Abstract:

Objective: To understand the typing of Rh system phenotypes and analyze the positive rate of irregular antibody in Rh system,and to provide basis for blood transfusion of the elderly patients in clinic.Methods: A total of 587 elderly blood tranfusion candidates were included. Microcolumn gel test was applied to detect the antigens of Rh system and identify the irregular antibody in Rh system.Results: There were 7 kinds of Rh blood group phenotypes with postive expression of RhD in 587 patients; the positive rates of irregular antibodies from high to low were CCDEE 38.7%(227/587),CcDEe 34.8%(204/587),CcDee 12.4%(73/587),ccDEE 8.7%(51/587),ccDEe 4.6%(27/587) and CCDEe 0.7%(4/587),ccDee 0.2%(1/587).There were 6 kinds of irregylar antibodies in 587 patients;the positive rate was 1.02%,including 4 cases of Anti-E (66.67%),1 case of Anti-c(16.67%),1 case of anti-M(16.67%).The postive rate of irregular antibody in the patients with blood transfution history and pregnancy history (2.65%,51/89) was higher than those without blood transfusion history or pregnancy history 0.25%,13/98)(χ²=5.11,P<0.05). There were no significant differences of the positive rates of irregular antibody between different nationalities(χ²=0.10,P>0.05).Conclusion: The irregular antibody of Rh system is the major antibody in the eldery blood transtusion candidates.The detection of typing of Rh system phenotypes and identification of irregular antibodies in the patients with blood transfusion history and pregnancy history should be performed to ensure the safty of blood transfusion.

Key words: Rh phenotype, microcolum gel test, irregular antibody