p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达及其意义

doi:10.13481/j.1671-587x.20170227

Abstract

Abstract: Objective: To detect the expressions of p16INK4a protein in the cervical lesion tissues of the Mongolian patients, and to explore the relationship between its expression and the occurrence and development of cervical cancer in the Mongolian patients. Methods: A total of 100 cases of paraffin sections of cervical cancer, cervical intraepithelial neoplasia(CIN),chronic cervicitis and uterine leiomyoma were divided into 25 cases of cervical cancer, 35 cases of CIN, 20 cases of chronic cervicitis, and 20 cases of uterine leiomyoma groups. The expressions of p16INK4a protein in different cervical tissues were detected by immunohistochemical SP method. Results: The positive rates of p16INK4a protern in cervical cancer, CIN, chronic cervicitis and uterine leiomyoma tissnes were 100.0%, 74.3%, 25.0%,and 10.0%, respectively.The results of K-WH rank sum test for multiple sample comparisons showed that the positive expression rate of p16INK4a protein in cervical cancer tissue was significantly higher than those in CIN, chronic cervicitis and uterine leiomyoma tissues(P<0.05). Conclusion: p16INK4a protein can be used as a indicator to screen the Mongolian patients with early cervical cancer.

Key words: INK4a gene, p16INK4a, cervical intraepithelial neoplasia, chronic cervicitis, cervical neoplasms, uterine leiomyoma

CLC Number:

R737.33

QIANG Xin, HUO Wenyan, YANG Can, FAN Yanan, SHI Caiyun, BAI Haihua. Expressions of p16INK4a protein in cervical lesion tissues of Mongolian patients and their significances[J].Journal of Jilin University Medicine Edition, 2017, 43(02): 353-355.

References

[1] 杨丽娟, 姚宇峰, 严志凌,等.HPV16型E6、E7变异与宫颈癌发生发展的研究进展[J].现代肿瘤医学, 2016, 24(11):1829-1832.
[2] Nam EJ, Kim JW, Kim SW, et al. The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance[J]. Gynecol Oncol, 2007, 104(1):207-211.
[3] Serrano M, Hannon GJ, Beach D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4[J]. Nature, 1993, 366(6456):704-707.
[4] Lau WM, Ho TH, Hui KM. p16INK4A-silencing augments DNA damage-induced apoptosis in cervical cancer cells[J]. Oncogene, 2007, 26(41):6050-6060.
[5] Kamijo T, Weber JD, Zambetti G, et al. Functional and physical interactions of the ARF tumor suppressor with p53 and Mdm2[J]. Proc Nat Acad Sci USA, 1998, 95(14):8292-8297.
[6] Zhang Y, Xiong Y, Yarbrough WG. ARF promotes MDM2 degradation and stabilizes p53:ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways[J]. Cell, 1998, 92(6):725-734.
[7] Zöchbauer-Müller S, Fong KM, Virmani AK,et al. Aberrant promoter methylation of multiple genes in non-small cell lung cancers[J]. Cancer Res, 2001, 61(1):249-255.
[8] Kanao H, Enomoto T, Ueda Y, et al. Correlation between p14(ARF)/p16 (INK4A) expression and HPV infection in uterine cervical cancer[J]. Cancer Lett, 2004, 213(1):31-37.
[9] Redman R, Rufforny I, Liu C,et al. The utility of p16(Ink4a) in discriminating between cervical intraepithelial neoplasia 1 and nonneoplastic equivocal lesions of the cervix[J]. Arch Pathol Lab Med, 2008,132(5):795-799.
[10] Omran OM, AlSheeha M. Human papilloma virus early proteins E6 (HPV16/18-E6) and the cell cycle marker P16 (INK4a) are useful prognostic markers in uterine cervical carcinomas in qassim Region- Saudi Arabia[J]. Pathol Oncol Res, 2015, 21(1):157-166.
[11] Samama B, Schaeffer C, Boehm N. P16 expression in relation to human papillomavirus in liquid-based cervical smears [J]. Gynecol Oncol, 2008, 109(2):285-290.
[12] 袁智英. TCT与HPV联合检测在宫颈癌筛查中的应用[J]. 公共卫生与预防医学, 2014, 25(2):58-60.
[13] Demir F, Kimiloglu E, Igdem AA, et al. High risk HPV in situ hybridization, p16 INK 4A, and survivin expressions in cervical carcinomas and intraepithelial neoplasms:evaluation of prognostic factors[J]. Eur J Gynaecol Oncol,2014, 35(6):708-717.
[14] 吕秀芳, 宋晓霞, 贺慧,等. p16免疫组化染色在宫颈癌筛查中的应用价值[J]. 河南医学研究, 2016, 25(8):1365-1367.
[15] 刘丽娜,李瑶,杨永秀,等.MCM5和p16^INK4A在宫颈鳞癌组织中的表达及其临床意义[J].西安交通大学学报:医学版,2015,36(6):829-835.
[16] 陈勇,周华蓉,黄玉秀,等.人乳头瘤病毒液态芯片基因分型和人乳头瘤病毒E6/E7mRNA检测的应用比较[J].中国老年学杂志,2016,35(7):1911-1913.

Expressions of p16INK4a protein in cervical lesion tissues of Mongolian patients and their significances

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	AN Le, YANG Jianzheng, HU Chunmei, YU Qiong, XIAO Han, HAO Shuhong, TANG Yan. Anti-apoptosis effect of polycystin-1 gene on human cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 776-779.
[2]	XU LI, ZHANG Wei. Induction of oxaziridine on apoptosis of human cervical cancer HeLa cells and its mechanism [J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1161-1164.
[3]	WEN Simin, YU Duo, LYU Xin, WANG Tiejun. Influence of docetaxel combined with cisplatinand single-agent cisplatin concurrent chemoradiotherapy in prognosis of patients with advanced cervical cancer and evaluation on security [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 1002-1008.
[4]	DENG Ziliang, WANG Sen, LI Peng, XIE Kuilong, LI Shuxian, ZHOU Shixiong, HE Xiaozhou, CHEN Dong, GUO Hongsheng. Enhancement of IL-37 in chemosensitivity of cervical cancer HeLa cells to cisplatin [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 862-866.
[5]	LIU Qianqian, SUN Lei, BIAN Li, HAN Jinhua, JIA Xiuxiu, YAO Xiaoqing, CHEN Yubing, SONG Xinling. Relationship between cognitive differences in medical behaviors and degrees of anxiety of cervical cancer patients undergoing radiotherapy [J]. Journal of Jilin University Medicine Edition, 2017, 43(01): 115-119.
[6]	XU Lu, ZENG Linchuan, YU Yang, DOU Minghan, LU Shibing, LI Songyan, XU Ye. Role of Ca²⁺ in cisplatin-induced autophagy of cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1045-1048.
[7]	ZHOU Jieping, WANG Peng, QIAN Liting, WU Aidong. Dosimetric comparison between IMRT and a single full arc,two full arc VMAT for postoperative cervical cancer patients [J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 120-124.
[8]	WANG Zhiliang, YU Tenghua, QIN Qin, WU Yutong, ZHANG Wenqian, HUA Yuanyuan, XIONG Zhengai, ZHOU Wei. Influence of irreversible electroporation mediated HPV16 E6 shRNA interference plasmid in proliferation of cervical cancer SiHa cells [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1107-1112.
[9]	ZHANG Wei, WU Yameng, CHEN Siyao, ZHANG Linna, HAN Jingchun, NA Wenting, JIN Ying. Effects of Juglone on proliferation and cell cycle of human cervical cancer SiHa cells [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 697-700.
[10]	YU Huiling, LI Dongmei, SUN Qinnuan, MA Hongxi. Expressions of apoptosis-related factors in serum and cervix tissue of patients with different cervical lesions and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2015, 41(01): 129-134.
[11]	XU Shan,YAN Meng-meng，LIU Yang，LI Peng-wu,MA Shu-mei,LIU Xiao-dong. Influence of miR-21 on radiation sensitivity of cervical cancer HeLa cells [J]. Journal of Jilin University Medicine Edition, 2014, 40(06): 1138-1143.
[12]	LIU Hong-li,LYU Chang-shuai,DING Bai-juan,WANG Jie,LI Shan,ZHANG You-zhong. Photodynamic effect of new photodynamic therapy combined with nucleolin silenceon cervical cancer cell line SiHa [J]. Journal of Jilin University Medicine Edition, 2014, 40(04): 748-752.
[13]	QI Si-qin,DE Sheng,LI Dong-mei,SHI Bo,HUANG Ke-xin. Expressions of PARP-1,Caspase-3,survivin and Bax in cervical squamous cell carcinoma tissues and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2013, 39(3): 512-516.
[14]	WANG Fu-xiang,PIAO Jin-xia,SU Qing-xiu,ZHANG Mao. Evaluation on efficacy of different doses of weekly cisplatin chemotherapy in treatment  of stage ⅡB－ⅣA cervical cancer [J]. Journal of Jilin University Medicine Edition, 2013, 39(2): 352-356.
[15]	WANG Hui-yan, CAI Hong-bing, CHEN Chang-chun, WANG Jing. Relationships between TGF-β1 expression\|HPV16 E6 gene polymorphism and squamous cervical carcinoma [J]. J4, 2012, 38(4): 750-754.