Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (06): 1045-1048.doi: 10.13481/j.1671-587x.20160601

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Role of Ca2+ in cisplatin-induced autophagy of cervical cancer HeLa cells

XU Lu1, ZENG Linchuan2, YU Yang1, DOU Minghan1, LU Shibing1, LI Songyan1, XU Ye1   

  1. 1. Center of Laboratory, School of Basic Medical Sciences, Jilin Medical College, Jilin 132013, China;
    2. School of Public Health, Jilin Medical University, Jilin 132013, China
  • Received:2016-04-11 Online:2016-11-28 Published:2016-12-02

Abstract:

Objective: To treat the cervical cancer HeLa cells by using calcium inhibitors combined with chemotherapy drugs,and to explore the effect of Ca2+ on autophagy in cancer cells,and to provide basis for tumor treatment and drug research.Methods: The human cervical cancer HeLa cells were at logrithmic growth phase randomly divided into control group,cisplatin group,2-APB group,and cisplatin + 2-APB group(combination group).MTT assay was used to measure the survival rates of HeLa cells; Ca2+-sensitive fluorescent dye was used to detect the levels of Ca2+ in cytoplasm and mitochondrial; the colocalization of LC3 and P62 were examined under confocal microscope.Results: The MTT assay results showed that compared with control group,the survival rates of HeLa cells in cisplatin and combination groups were signifcantly decreased(P<0.05).Compared with cisplatin group,the survival rate of HeLa cells in combination group was significantly increased(P<0.05).The Ca2+-sensitive fluorescent dye detection results showed that compared with cisplatin group,the mitochondrial free Ca2+ level and the fluorescence intensity of colocalization of LC3 and P62 protein in combination group were significantly decreased.Conclusion: Chemotherapy drugs can induce the autophagy of tumor cells,and calcium inhibitors can increase the sensitity of tumor cells to chemotherapy drugs by reducing the autophagy levels.

Key words: cisplatin, calcium ion, autophagy, cervical neoplasms

CLC Number: 

  • R737.33