表皮生长因子受体酪氨酸激酶抑制剂厄洛替尼联合山奈酚诱导卵巢癌细胞凋亡的协同机制

doi:10.13481/j.1671-587x.20160107

Abstract

Abstract:

Objective: To study the inhibitory effect of epidermal growth factor receptor tyrosine kinase(EGFR-TPK),and to clarify the mechanism of their synergetic inhibitory effects on the growth of ovarian cancer cells. Methods: The human ovarian cancer SKOV 3 cells were cultured and divided into blank group,kaempferol group and tarceva group. The activities of EGFR-TPK were detected by ELISA method.The invasion abilities of SKOV-3 cell lines were determined by Transwell assay. The caspase-3 activities of SKOV-3 cells were measured. The inhibitory rates of growth of SKOV-3 cells were determined using MTT method. Results: The half inhibitory concentrations (IC₅₀)of kaempferol and tarceva were (52.33±0.32)g·L^-1and (1.75±0.18)g·L^-1,there was no significant differences between them(P>0.05).The inhibitory rates of growth of SKOV-3 cells were increased with the increasing of doses of kaempferol and tarceva,and there were significant differences compared with blank group(P<0.05 or P<0.01). The caspase-3 activities of SKOV-3 cells of in kaempferol and tarceva groups were significantly increased compared with blank group(P<0.05). Conclusion: Kaempferol in combination with tarceva has a synergetic effect on the growth of ovarian cancer SKOV-3 cells.

Key words: kaempferol, tarceva, epidermal growth factor receptor tyrosine kinase, ovarian cancer SKOV-3 cells, inhibitory effect, apoptosis, caspase-3

CLC Number:

R737.31

ZHANG Baoyue, ZHANG Junnong. Synergetic effect of epidermal growth factor receptor tyrosine kinase inhibitor kaempferol in combination with tarceva on apoptosis of ovarian cancer cells[J].Journal of Jilin University Medicine Edition, 2016, 42(01): 31-35.

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Synergetic effect of epidermal growth factor receptor tyrosine kinase inhibitor kaempferol in combination with tarceva on apoptosis of ovarian cancer cells

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