柔红霉素对人B细胞淋巴瘤OCI-LY7细胞凋亡的诱导作用及其机制

doi:10.13481/j.1671-587x.20190625

Abstract

Abstract: Objective: To investigate the effect of daunorubicin on the apoptosis of human B-cell lymphoma OCI-LY7 cells and its mechanism,and to elucidate the drug resistance mechanism of daunorubicin in the diffuse large B-cell lymphoma. Methods: The OCI-LY7 cells cultured in vitro were divided into 0,0.1,1.0 and 10.0 mg·L^-1 daunorubicin groups.The survival rates of OCI-LY7 cells in various groups were detected by MTT assay,the apoptotic rates of the OCI-LY7 cells in various groups were detected by flow cytometry,and the expression levels of apoptosis-related proteins Bax,cleaved caspase-3, and survivin in the OCI-LY7 cells in various groups were detected by Western blotting method. The follow-up experiments were divided into control group,daunorubicin group,daunorubicin+vector group and daunorubicin+survivin group; the survival rates,the apoptotic rates and the expression levels of apoptosis-related proteins in the OCI-LY7 cells in various groups after overexpression of survivin were detected by the above-mentioned methods. Results: Compared with 0 mg·L^-1 group,the survival rates of OCI-LY7 cells and the expression levels of survivin protein in the OCI-LY7 cells in 0.1,1.0 and 10.0 mg·L^-1 daunorubicin groups were decreased gradually after treated for 48 h(P<0.05),while the apoptotic rates and the expression levels of Cleaved caspase-3 and Bax proteins in the OCI-LY7 cells were gradually increased (P<0.05). Compared with control group,the survival rate and the expression level of survivin protein in the OCI-LY7 cells in daunorubicin group were significantly decreased(P<0.05),while the apoptotic rate and the expression levels of cleaved caspase-3 and Bax proteins in the OCI-LY7 cells were significantly increased (P<0.05); compared with daunorubicin group,the survival rate and the expression level of survivin protein in the OCI-LY7 cells in daunorubicin+survivin group were significantly increased(P<0.05),while the apoptotic rate and the expression levels of cleaved caspase-3 and Bax protein in the OCI-LY7 cells in daunorubicin+vector group were significantly decreased (P<0.05). Conclusion: Daunorubicin can induce the apoptosis of OCI-LY7 cells,and its mechanism may be related to down-regulation of the expression of survivin protein.

Key words: human B cell lymphoma, daunorubicin, apoptosis, survivin

CLC Number:

R733.4

TIAN Pengfei, CHEN Juanjuan, YIN Junjie, LI Bing, GUO Shan. Inductive effect of daunorubicin on apoptosis of human B-cell lymphoma OCI-LY7 cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1340-1345.

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Inductive effect of daunorubicin on apoptosis of human B-cell lymphoma OCI-LY7 cells and its mechanism

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