复方苦参注射液对鼻咽癌CNE-2细胞的放射增敏作用

doi:10.13481/j.1671-587x.20190632

Abstract

Abstract: Objective: To observe the effect of Compound Sophora Flavescens Injection on the radiosensitivity of the human nasopharyngeal carcinoma CNE-2 cells,and to explore its mechanism. Methods: The human nasopharyngeal carcinoma CNE-2 cells were cultured in vitro. The inhibitory rates of proliferation of CNE-2 cells after treated with different concentrations of Compound Sophora Flavescens Injection for 24, 48, and 72 h were detected by MTT method. The semi-inhibitory concentration (IC₅₀) of Compound Sophora Flavescens Injection on CNE-2 cells was calculated. The CNE-2 cells were divided into control group(without irradiation and adminnistration), irradiation group (treated with 2 and 4 Gy X-ray irradiation) and combination group(treated with different concentrations of Compound Sophora Flavescens Injection and then received X-ray irradiation). MTT assay was used to detect the inhibitory rates of proliferation of the CNE-2 cells in various groups, flow cytometry was used to detect the apoptotic rates of the CNE-2 cells in various groups,and clone formation test was used to detect the survival rates of the CNE-2 cells in various groups. Results: After treated with different concentrations of Compound Kushen Injection for 24, 48 and 72 h, the inhibitory rates of proliferation of the CNE-2 cells were increased compared with the CNE-2 cells without Compound Kushen Injection treatment (P<0.05) in an dose-dependent manner;the inhibitory rate of proliferation of the CNE-2 cells after treated with Compound Kushen injection for 48 h was higher than 24 h (P<0.05).The apoptotic rate of the CNE-2 cells in radiation group was higher than that in control group (P<0.05); the apoptotic rate of the CNE-2 cells in combination group was higher than that in radiation group (P<0.05);the survival rate of the CNE-2 cells in combination group was lower than that in radiation group(P<0.05). Conclusion: Compound Sophora Flavescens Injection can promote the irradiation-induced apoptosis of nasopharyngeal carcinoma CNE-2 cells, therefore, Compound Sophora Flavescens Injection has the radiosensitization effect on the CNE-2 cells.

Key words: Compound Sophora Flavescens Injection, apoptosis, radiosensitization, nasopharyngeal neoplasms

CLC Number:

R739.63

ZHENG Jianxiao, ZHAN Wenting, ZHOU Xiaochun, KONG Yilin, GAO Lei, LI Gong, WANG Sumei. Radiosensitization of Compound Sophora Flavescens Injection on nasopharyngeal carcinoma CNE-2 cells[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1384-1388.

References

[1] TANG L L, CHEN W Q, XUE W Q, et al. Global trends in incidence and mortality of nasopharyngeal carcinoma [J]. Cancer Lett,2016, 374(1): 22-30.
[2] MARKS J E, BEDWINEK J M, LEE F, et al. Dose response analysis fornasopharyngeal carcinoma: an historical perspective [J]. Cancer,1982, 50(6): 1042-1050.
[3] HSU H C, CHEN C L, HSU M M, et al. Pathology of nasopharyngeal carcinoma. Proposal of a new histologic classification correlatedwith prognosis [J]. Cancer, 2015, 59(5): 945-951.
[4] SU S F, HAN F, ZHAO C, et al. Treatment outcomes for different subgroups of nasopharyngeal carcinoma patients treated with intensity-modulated radiation therapy [J]. Chin J Cancer,2011, 30(8): 565-573.
[5] 李博, 康敏, 陆颖, 等. 鼻咽癌调强放射治疗后局部与区域复发模式[J].中国癌症防治杂志,2018, 10(2):124-128.
[6] MU XQ, MA J, ZHANG ZJ,et al.Famitinib enhances nasopharyngeal cancer cell radiosensitivity by attenuating radiation-induced phosphorylation of platelet-derived growth factor receptor and c-kit and inhibiting microvessel formation [J]. Int J Radiat Biol,2015, 91(9):771-776.
[7] SUN Z Y, PAN X, ZOU Z W,et al.Increased SHP-1 expression results in radioresistance,inhibition of cellular senescence,and cell cycle redistribution in nasopharyngeal carcinoma cells [J]. Radiat Oncol, 2015,10:152.
[8] 肖冰. 复方苦参注射液对宫颈癌患者免疫功能及炎症反应的影响[J].内蒙古中医药,2019,38(2):91-92,97.
[9] 张志聪,张慧芝,万永红. 复方苦参注射液对宫颈鳞癌放疗增敏效果观察和评价[J].大同大学学报:自然科学版,2018, 34(5):51-53.
[10] PIEME C A, SANTOSH G K,TEKWU E M, et al. Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and G₀/G₁ phase arrest in human leukemia HL-60 cells [J]. Biol Res, 2014, 47(1):54-59.
[11] 陈红红, 莫书荣,吴忧. 黄芪注射液对人鼻咽癌CNE-1细胞增殖、凋亡及细胞周期阻滞的影响研究[J]. 广西医科大学学报,2019,36(1):10-14.
[12] 董俊,晏菲,邓洁,等. 复方苦参注射液抗肿瘤机制及其临床应用进展[J]. 天津医学, 2019, 31(1): 71-74.
[13] 袁选举, 王贤和. 复方苦参注射液对宫颈癌细胞体外放射增敏作用研究[J]. 现代中西医结合杂志, 2016, 25(23):2509-2511,2525.
[14] 张均田. 现代药理实验方法[M]. 2版. 北京: 北京医科大学、中国协和医科大学联合出版社,1998: 952-953.
[15] MEYN R E, STEPHENS L C, ANG K K, et al. Heterogeneity in the development of apoptosis in irradiated murine tumours of different histologies [J]. Int J Radiat Biol,1993,64(5):583-591.
[16] VELLANKI S H,GRABRUCKER A,LIEBAU S,et al. Small-molecule XIAP inhibitors enhance gamma-irradiation induced apoptosis in glioblastoma [J]. Neoplasia,2009,11(8): 743-752.
[17] 刘国慧,谷安鑫,殷洪涛,等. 原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究[J]. 实用肿瘤学杂志,2017, 31(6): 500-505.
[18] 钟晓鸣, 邬蒙. 鼻咽癌中自发凋亡与临床放射敏感性的研究[J]. 实用癌症杂志, 2008, 23(6): 619-621.
[19] 王若峥, 吴冉, 黄莉, 等. 人鼻咽癌细胞株自发性凋亡与放射敏感性的研究[J]. 中国肿瘤临床,2013,40(9):491-495.
[20] 胡万宁,王晓红,王建功,等.复方苦参注射液体外诱导鼻咽癌CNE-2细胞凋亡的作用及机制[J].山东医药,2011,51(9):92-93.
[21] 陆佳扬,黄宝添,李梅,等.应用放射生物学模型比较早期鼻咽癌不同放疗剂量分割方案[J].中国医学物理学杂志,2019,36(6):626-631.

Radiosensitization of Compound Sophora Flavescens Injection on nasopharyngeal carcinoma CNE-2 cells

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