Abstract: Objective: To observe the expressions of heparanase(HPA) protein and integrin α5β1 protein in the human benign and malignant hydatidform mole tissues,and to elucidate their roles in the occurrence and development of benign and malignant hydatidiform moles. Methods: A total of 50 cases of normal placental villus tissue (normal placental villus group),40 cases of benign hydatidiform moles tissue (benign hydatidform mole group) and 17 cases of malignant hydatidiform mole tissue (malignant hydatidform mole group) were selected. The positive expression rates and expression intensities of HPA protein and integrin α5β1 protein in tissues in various groups were detected by immunohistochemical SP method, the expression levels of HPA protein and integrin α5β1 proteins in tissues in various groups were detected by Western blotting method; Spearman correlation method was used to analyze the correlation between the expressions of HPA protein and integrin α5β1 protein. Results: The positive expression rates,the expression intensities and the expression levels of HPA and integrinα5β1 in tissues in benign hydatidiform mole group and malignant hydatidiform mole group were higher than those in normal placental villus group(P<0.05); the positive expression rate,the expression intensity, and the expression levels of HPA protein and integrin α5β1 protein in tissue in malignant hydatidiform mole group were higher than those in benign hydatidiform mole group(P<0.05).There were positive correlations between the expressions of HPA protein and integrin α5β1 protein in both benign and malignant hydatidiform mole tissues(r=0.506,P<0.05;r=0.555,P<0.05). Conclusion: The combined detection of the expressions of HPA protein and integrin α5β1 protein may become a predictive indicator for the differential diagnosis of benign hydatidiform mole and malignant hydatidiform mole.

Key words: hydatidform mole, immunohistochemistry, Western blotting method, heparanase, integrin α5β1