Abstract: Objective: To perform the function experiment in the pathogenic candidate gene TP53 via cell experiment,and to investigate the effects of TP53 G199X and V157fs point mutations on the protein expression, proliferation, migration and invasion of the ovarian cancer A2780 cells. Methods: The ovarian cancer A2780 cells without endogenous TP53 gene expression were selected and divided into wild-type group, G199X group, V157fs group and empty vector group. The TP53 wild-type plasmid was constructed, and the two point mutation plasmids of G199X and V157fs were constructed by using the point mutation kit. The wild-type, mutant and empty vector plasmids were transfected into the A2780 cells by lipofection. Western blotting method was used to detect the the expressions of P53 protein in the A2780 cells in various goups. The proliferation activities of A2780 cells in various groups were detected by CCK-8 assay. The rates of scratch healing of A2780 cells in various groups were detected by scratch assay. Transwell assay was used to examine the number of invasion cells of A2780 cells in various groups. Results: After transfection of A2780 cells, the P53 protein didn't express in the cells in G199X group, V157fs group and empty vector group. Compared with wild-type group, the proliferation activities of the cells in G199X group,V157fs group and empty vector group were significantly increased(P<0.01);the scratch healing rates of the cells in G199X group and V157fs group were increased (P<0.05 or P<0.01); the number of invasion cells in G199X group,V157fs group and empty vector group was increased(P<0.01). Conclusion: Two point mutations of TP53 G199X and V157fs can stop the expression of P53 protein in the A2780 cells and promote the proliferation, migration and invasion of ovarian cancer A2780 cells.

Key words: TP53 gene, point mutation, cell proliferation, cell migration, cell invasion