姜黄素预处理对急性缺血再灌注模型大鼠肝脏的保护作用

doi:10.13481/j.1671-587x.20200215

Abstract

Abstract: Objective: To establish the acute liver ischemia-reperfusion models of rats pre-treated with curcumin, and to investigate the protective effect of curcumin on the liver of the rats after acute ischemia-reperfusion. Methods: Ten SD rats were randomly assigned to sham operation group(n=3),solvent control group(n=3) and curcumin group(n=4).The rats in curcumin group and solvent control group were pre-treated with curcumin and 1% carboxymethyl cellulose (CMC) respectively by intraperitoneal injection before acute liver ischemia-reperfusion. The rats in sham operation group did not receive acute liver ischemia-reperfusion. Ultra performance liqwid chromatography-mass specteum(UPLC-MS) was used to determine the levels of serum curcumin of the rats in various. Bile duct cannulation and flurescence analysis were used to measure the bile flows of the rats in various groups. The malondialdehyde (MDA) kit was used to detect the MDA levels in liver tissue of the rats in various groups. Results: The serum curcumin level of the rats 1 h after introperitoneal injection of 200 mg·kg^-1 curcumin reached to(0.17±0.05)mg·L^-1, but decreased to below the detection level at 3 h after injection. The MDA level in liver tissue of the rats in curcumin group (9.18mmol·g^-1±1.78mmol·g^-1) was lower than those in solvent control group (527.54mmol·g^-1±237.38mmol·g^-1) and sham operation group (162.73mmol·g^-1±90.50mmol·g^-1).The bile flow of the rats in curcumin group recovered to approximately 40% of basal flow after ischemia-reperfusion, and had no significant difference compared with solvent control group (P>0.05).The bile flow of the rats in sham operation group kept in the basal flow level. Conclusion: Curcumin could be readily detected in the blood of the rats after introperitoneal injection; it is metabolized rapidly in vivo, and its level could decrease to below the detection level at 3 h after administration. Pre-treatment with curcumin can protect the liver by decreasing the MDA level in liver tissue of the rats after liver ischemia-reperfusion. However the treatment has the limited effect on bile flow restoration.

Key words: curcumin, liver, ischemia-reperfusion, bile duct cannulation, malondialdehyde

CLC Number:

R285.5

ZHOU Yabin, HUA Jin, QI Lingli, DAI Lu, PANG Xiaoli. Protective effect of curcumin pre-treatment on liver of acute ischemia-reperfusion model rats[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 297-301.

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Protective effect of curcumin pre-treatment on liver of acute ischemia-reperfusion model rats

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