CpG ODN BW006对实验性牙移动模型大鼠牙周组织中Runx2和Osterix表达的影响及其意义

doi:10.13481/j.1671-587x.20200222

Abstract

Abstract: Objective: To observe the effect of immunostimulatory oligodeoxynucleotide (CpG ODN) BW006 on the expressions of Runt-related transcription factor 2(Runx2) and Osterix in periodontal tissue of the rats with experimental tooth movement, and to explore its effect on the periodontal remodeling of the rats with orthodontic tooth movement. Methods: The experimental tooth movement models were established in the 36 7-week-old male Wistar rats. The left maxilla was used as experimental group (local injection of CpG ODN BW006) and the right maxilla as control group (local injection of PBS), once every 3 d, 40 μL each time. Twelve rats were randomly selected at 3, 7 and 14 d after stress, and the maxillary tissue containing the first molars was prepared. HE staining and immunohistochemical staining were performed to observe the morphological changes of periodontal tissue, measure the tooth movement distance and detect the expressions of Runx2 and Osterix. Results: The HE staining results showed that the bone resorption on the pressure side of the rats in experimental group was lower than that in control group, and a large number of osteoblasts were seen on the tension side of the rats in experimental group; the alveolar bone fullness and height of the rats in experimental group were significantly higher than those in control group. The distances of tooth movement in experimental group were (0.347±0.007) and (0.443±0.016)mm, while they were (0.585±0.012) and (0.975±0.084)mm in control group at 7 and 14 d; there were significantly differences between two groups(P<0.01). The immunohistochemical staining results showed that the positive expression levels of Runx2 and Osterix in the periodontal tissue of the rats in experimental group were significantly higher than those in control group at 3 and 7 d (P<0.05 or P<0.01), and reached a peak on the 7th day; the positive expression levels of Runx2 and Osterix in experimental group were decreased slightly on the 14th day, but there were still significant differences compared with control group (P<0.05 or P<0.01). Conclusion: CpG ODN BW006 can regulate the periodontal tissue remodeling by promoting the expressions of Runx2 and Osterix during tooth movement.

Key words: immunostimulatory oligodeoxynucleotide, Runt2, Osterix, periodontal tissue remodeling

CLC Number:

R783.5

HUANG Lei, LIU Yuyan, YU Wenwen, ZHOU Xuechun, ZHANG Xiao, SHEN Yuqin, ZHENG Yi, SUN Xinhua. Effect of CpG ODN BW006 on expressions of Runx2 and Osterix in periodontal tissue of experimental tooth movement model rats and its significance[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 340-345.

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Effect of CpG ODN BW006 on expressions of Runx2 and Osterix in periodontal tissue of experimental tooth movement model rats and its significance

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