MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

doi:10.13481/j.1671-587x.20170418

Abstract

Abstract: Objective: To investigate the changes of expression levels of TLR9 and IL-6 in the periodontal tissue during the experimental tooth movement of the rats, and the effecst of the MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue,and to clarify its related mechanisms.Methods: Thirty Wistar rats were randomly divided into MT01 intervention group(n=6) and non-MT01 group(n=24). Force of 0.49 N was applied to move the upper first molars mesially. The rats in Non-MT01 intervention group were sacrificed on the days 3,7,14 and 21, and the rats in MT01 intervention group were all sacrificed on the day 7. RT-qPCR was used to detect the expression levels of TLR9,TRAF6 and IL-6 mRNA in maxillary first molar alveolar bone in each group.Results: The expression levels of IL-6 and TLR9 mRNA in loaded side were higher than those in control side(P<0.05), and reached the maximum levels on the day 7(P<0.01); with the interpose of MT01, the expression levels of TLR9 and TRAF6 mRNA were lower than control side(P<0.01).Conclusion: MT01 could down-regulate the expression levels of TLR9 and TRAF6 during orthodontic tooth movement and eventually resists the inflammation during the tooth movement.

Key words: MT01, tooth movement, tumor necrosis factor receptor associated factor 6, toll like receptors 9, interleukin-6, polymerase chain reaction

CLC Number:

R783.5

YANG Zhujun, HOU Xu, YU Wenwen, YAN Kuo, SHEN Yuqin, SUN Xinhua. Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats[J].Journal of Jilin University Medicine Edition, 2017, 43(04): 752-756.

References

[1] Graves DT, Kayal RA, Oates T, et al. Osteoimmunology in the oral cavity (periodontal disease, lesions of endodontic origin and orthodontic tooth movement)[J]. Osteoimmunology, 2016:325-344.
[2] Di DM, D'Apuzzo F, Feola A, et al. Cytokines and VEGF induction in orthodontic movement in animal models[J]. Biomed Res Int, 2012, 2012(5):33-36.
[3] Zhang D. A toll-like receptor that prevents infection by uropathogenic bacteria[J]. Science, 2004, 303(5663):1522-1526.
[4] Lacerte P, Brunet A, Egarnes B, et al. Overexpression of TLR2 and TLR9 on monocyte subsets of active rheumatoid arthritis patients contributes to enhance responsiveness to TLR agonists[J]. Arthri Res Ther, 2016, 18(1):1-14.
[5] Devaraju P, Gulati R, Antony PT, et al. Susceptibility to SLE in South Indian Tamils may be influenced by genetic selection pressure on TLR2 and TLR9 genes[J]. Mol Immunol, 2015, 64(1):123-126.
[6] Rao H, Zeng Q, Liang Y, et al. Correlation between TLR9 expression and cytokine secretion in the clinical diagnosis of systemic lupus erythematosus[J]. Mediators Inflamm, 2015, 2015:710720.
[7] Yang G, Wan M, Zhang Y, et al. Inhibition of a C-rich oligodeoxynucleotide on activation of immune cells in vitro, and enhancement of antibody response in mice[J]. Immunology, 2010, 131(4):501-512.
[8] Xu H, Yuqin S, Chao Z, et al. A Specific oligodeoxy nucleotide promotes the differentiation of osteoblasts via ERK and p38 MAPK pathways[J]. Int J Mol Sci, 2012, 13(12):7902-7914.
[9] 王静, 孙新华, 冯志远,等. 特定寡核苷酸对大鼠实验性牙移动的影响[J]. 吉林大学学报:医学版, 2011, 37(5):812-816.
[10] Meeran NA. Cellular response within the periodontal ligament on application of orthodontic forces[J]. J Indian Soc Periodontol, 2013, 17(1):16-20.
[11] Nokhbehsaim M, Deschner B, Winter J, et al. Contribution of orthodontic load to inflammationmediated periodontal destruction[J]. For Der Kie, 2010, 71(6):390-402.
[12] Liang J, Luan Y, Lu B, et al. Protection of ischemic postconditioning against neuronal apoptosis induced by transient focal ischemia is associated with attenuation of NF-κB/p65 activation.[J]. PLos One, 2014, 9(5):1-7.
[13] Liu Z, He D, Zhang X, et al. Neuroprotective effect of early and short-time applying sophoridine in pMCAO rat brain:down-regulated TRAF6 and up-regulated p-ERK1/2 expression, ameliorated brain infaction and edema[J]. Brain Res Bull, 2012, 88(4):379-384.
[14] 米丛波, 刘红, 钱雅婧,等. 正畸力作用下大鼠牙周组织中肝细胞生长因子的表达[J]. 牙体牙髓牙周病学杂志, 2010(11):622-625.
[15] 靳玫,刘晓利,唐超智,等.Toll样受体在大鼠大脑皮层发育过程中的表达[J].郑州大学学报:医学版,2017,52(1):41-44.
[16] Yang JH, Li ZC, Kong WD, et al. Effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats[J]. Asian Pac J Tropl Med, 2013, 6(10):757-761.
[17] 冯志远. 寡核苷酸(ODN)促成骨细胞增殖活化的作用及机制研究[D]. 长春:吉林大学, 2011.

Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHENG Yan, WANG Na, WU Mingkun, LIU Jingqiao, MENG Yali. Influence of mifepristone in expression levels of Ang-1,Ang-2, VEGF, TNF-α and IL-6 in serum and villus tissue of patients with missed abortion [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 791-795.
[2]	GAO Lijun, HE Chengyuan, LI Yingnuo, BA Hongyu, LI Zitong, XIA Wei, LI Mingcheng, YUAN Guanxin, ZHANG Lihua, AI Jinxia. Characteristics and identification of DNA fingerprint of velvet antler and its counterfeits based on duplex PCR technique [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 839-844.
[3]	HUANG Baoliang, DING Chuanbo, WANG Jiaqi, ZHENG Yinan, LIU Wencong, ZHANG Jing, XU Xiaohua. Anti-fatigue effect of arginylfructosylglucose from red ginseng in mice [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 897-902.
[4]	HANG Min, REN Hongwei, QU Huiming, GENG Long. Clinical significance of IL-17 expression in serum of patients with psoriasis vulgaris and effects of shikonin on IL-6 and IL-23 production in IL-17-stimulated HaCaT cells [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 958-962.
[5]	LU Guoxiu, ZHANG Guoxu, HAO Shanhu, GUO Jia, ZHANG Wenwen, ZHANG Jinghua, WANG Zhiguo. PET/CT imaging and detection of levels of IL-6 and expression rates of ApoE in Alzheimer's disease patients with different body-mass indexes and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 611-614.
[6]	WEN Aiping, WANG Haijiao, JIN Jingji, CAI Yong, ZHU Jihong, CHEN Lunhua. Expressions of hMOFprotein and mRNA in endometrial cancer tissue and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1138-1142.
[7]	LIU Huanxin, ZHANG Guoxiang, GUO Linlang, TAN Songshan. Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 942-948.
[8]	LIU Yin, SHEN Yuqin, GAO Han, FEI Hongbo, HU Tianqi, LI Yangyang, GU Zhongyi, LIN Chongtao. Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance [J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 681-684.
[9]	WEN Jianping, CUI Yongsheng, ZHANG Xiongji. Expression levels of IL-6 and VEGF in lung adenocarcinoma cells transfected with EGFRL858R and their significances [J]. Journal of Jilin University Medicine Edition, 2016, 42(03): 502-505.
[10]	CUI Yue, JIANG Huan, CUI Congcong, HU Min. Ease effects of local injection of ursolic acid on orthodontic tooth movement and root resorption of rats and their mechanisms [J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 231-235.
[11]	DONG Zefeng, ZHENG Tianli, FAN Xuejia, Pei Xiaofang. Epidemic characteristics of rhinovirus in hospitalized children with respiratory syndrome in Chengdu during 2011-2013 [J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 177-181.
[12]	SUN Xiaohong, FANG Fuyuan, LI Hui, YIN Na, PANG Zuoliang, LI Huiwu. Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 814-819.
[13]	PENG Jing, YUAN Ruili, WANG Xiaoqin, WU Feng, GUO Xuan. Detection of expression levels of microRNA-181a and microRNA-20a in serum of patients with multiple myeloma and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 625-630.
[14]	ZHANG Wenjing, JING Hongyu, XU Hongbang, QIAN Donghua. Analysis on association between polymorphisms of IL-4-33C/T and IL-6-572C/G and susceptibility of chronic obstructive pulmonary disease [J]. Journal of Jilin University Medicine Edition, 2015, 41(01): 145-149.
[15]	ZHAO Min-xue，LI Bai-wen，ZHU Lin，YE Hong-shan，TIAN Xiao-feng，WEI Liang，CAO Hong. Detection of miR-21 expression levels in carcinoma tissue and serum of patients with papillary thyroid carcinoma and its significance [J]. Journal of Jilin University Medicine Edition, 2014, 40(06): 1237-1240.