含缬酪肽蛋白在上皮性卵巢癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20200327

Abstract

Abstract: Objective: To observe the expression of valosin-containing protein(VCP) in the epithelial ovarian cancer (EOC) tissue and its relationships with the clinicopathological features of the EOC patients,and to provide the basis for the molecular treatment of EOC. Methods: The expressions of VCP in 94 EOC tissue samples,13 ovarian borderline tumor tissue samples,36 ovarian benign tumor tissue samples and 8 normal ovarian tissue samples were measured by immunohistochemical method. The relationships between the VCP expression and the clinicopathological parameters (age,FIGO stage,pathological type,histological grade, lymph node metastasis or not, ascites or not, preoperative CA125 level) of the EOC patients were analyzed.The expressions of VCP in human ovarian epithelial HOSEPIC cells and human EOC SKOV-3 cells were detected by immunofluorescence technique and Western blotting method.The SKOV-3 cells were divided into control group(without CB-5083) and treatment group (with CB-5083), the migration rates of the SKOV-3 cells in two groups were analyzed by scratch experiment, and the clone formation rates of the SKOV-3 cells in two groups were analyzed by plate clone formation experiment. The expressions of VCP in the cells in two groups were analyzed by immunofluorescence technique.The expression levels of VCP, NF-κB/P65,IκBα,and p-IκBα proteins in the SKOV-3 cells in two groups were detected by Western blotting method. Results: The positive expression rates of VCP in EOC, borderline ovarian tumor, benign ovarian tumor and normal ovarian tissues had significant difference (P<0.05);the expression of VCP was associated with the FIGO staging, histological grade, and lymph node metastasis or not(P<0.05), but was not associated with the age, pathological type, ascites or not, and preoperative Ca125 level of the EOC patients (P>0.05).The VCP expression level in the SKOV-3 cells was higher than that in HOSEpiC cells(P<0.05).The migration rate of the SKOV-3 cells in treatment group was lower than that in control group (P<0.05),the cloning formation rate of the SKOV-3 cells in treatment group was lower than that in control group(P<0.05).The expression levels of VCP and NF-κB/P65 proteins of the SKOV-3 cells in treatment group were lower than those in control group (P<0.05),and the expression level of p-IκBα protein was higher than that in control group (P<0.05). Conclusion: VCP is highly expressed in the EOC tissue and cells, and high-expression VCP can promot the tumor migration and proliferation.

Key words: ovarian neoplasm, valosin-containing protein, scratch experiment, plate clone formation experiment

CLC Number:

R737.31

GUO Xiaofei, LIU Lihong, AI Hao. Expression of valosin-containing protein in epithelial ovarian cancer tissue and its clinical significance[J].Journal of Jilin University(Medicine Edition), 2020, 46(03): 600-606.

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Expression of valosin-containing protein in epithelial ovarian cancer tissue and its clinical significance

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