下调ADAR1表达对人肺癌细胞增殖、迁移和上皮间质转化的影响

doi:10.13481/j.1671-587x.20200401

Abstract

Abstract: Objective: To investigate the effects of down-regulation of adenosine deaminase acting on RNA enzyme 1 (ADAR1) on the proliferation, migration and epithelial-mesenchymal transition (EMT) of the lung cancer H1299 and H520 cells, and to elucidate their mechanisms. Methods: The H1299 and H520 cells were divided into control group (transfected with negative shRNA) and shADAR1 group (transfected with shADAR1). Real-time fluorescence quantitative PCR and Western blotting methods were used to detect the expression levels of ADAR1 mRNA and protein, respectively. CCK8 method and colony information assay were used to detect the proliferation activities and the amounts of colony formation;scratch assay was used to detect the scratch healing rates of the cells in various groups; Western blotting method was performed to detect the expression levels of E-cadherin and vimentin proteins in the cells in various groups. Results: Compared with control group, the expression levels of ADAR1 mRNA and protein in the H1299 and H520 cells in shADAR1 group were significantly decreased(P<0.05).Compared with control group, the proliferation activity, the colony formation amount and the scratch healing rate of the H1299 and H520 cells in shADAR1 group were significantly decreased (P<0.05).Compared with control group, the expression levels of E-cadherin protein in the H1299 and H520 cells in shADAR1 group were significantly increased(P<0.01), and the expression levels of vimentin protein were decreased (P<0.01). Conclusion: Down-regulation of ADAR1 expression contributes to inhibiting the proliferation, migration, and EMT of lung cancer cells.

Key words: adenosine deaminase acting on RNA enzyme 1, lung neoplasms, cell proliferation, cell migration, epithelial-mesenchymal transition

CLC Number:

R734.2

ZHOU Ning, WU Rui, MA Zhenkai, CHEN Weiwei, LI Xuelin, GONG Kaikai, YANG Lijuan, DAI Juanjuan, WU Yan. Effects of down-regulation of ADAR1 expression on proliferation, migration and epithelial-mesenchymal transition of human lung cancer cells[J].Journal of Jilin University(Medicine Edition), 2020, 46(04): 669-674.

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Effects of down-regulation of ADAR1 expression on proliferation, migration and epithelial-mesenchymal transition of human lung cancer cells

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