IL-17A对前列腺癌细胞迁移的促进作用及其机制

doi:10.13481/j.1671-587x.20200614

Abstract

Abstract: Objective

To observe the effect of interleukin-17A-signal transducer and activator of transcription 3-vascular endothelial growth factor（IL-17A-Stat3-VEGF） signaling pathway activation on the migration of prostatic cancer cells， and to explore the relative mechanism.

Methods

A total of 73 prostatic samples including 8 cases of normal prostate （NP） tissue （adjacent normal tissue）， 30 cases of benign prostatic hyperplasia （BPH） tissue and 35 cases of prostate cancer tissue. Immunohistochemical staining method was used to detect the expressions of IL-17A， IL-17RA， Stat3 and VEGF in NP， BPH and prostate cancer tissues， and their correlations with the malignancy degrees of prostate cancer were analyzed.ELISA method was used to detect the serum levels of IL-17A， IL-6， VEGF and matrix metallopeptidase-9 （MMP-9） of the subjects in healthy control group and prostate cancer group. The prostatic cancer cells were cultured with IL-17A recombinant protein in vitro， and scratching test and Transwell chamber experiment were used to observe the migration ability of the cells；Western blotting method was used to detect the expression levels of Stat3， p-Stat3 and VEGF in the PC3 cells in various groups.

Results

The immunohistochemical staining results showed that the expression levels of IL-17A and IL-17RA in BPH tissue were significantly increased compared with NP tissue（P<0.05）； the expression levels of IL-17A， IL-17RA， Stat3， and VEGF in prostate cancer tissue were higher than those in NP tissue（P<0.05）. Compared with BPH tissue， the expression levels of IL-17A， IL-17RA， Stat3， and VEGF in prostate cancer tissue were increased （P<0.05）. The ELASA results showed that the serum levels of IL-17A， IL-6，and VEGF in the patients with prostate cancer were significantly higher than those in healthy controls（P<0.05）. In the prostate cancer tissue， the expression levels of IL-17A， IL-17RA， Stat3， and VEGF were significantly increased with the increasing of Gleason score （P<0.05）. After the PC3 cells were co-cultured with IL-17A recombinant protein for 24 h， the number of migration cells in 50 and 100 μg · L^－1 IL-17A groups was significantly increased compared with blank control group （0 μg · L^－1 IL-17A）（P<0.05）；the expression levels of Stat3，p-Stat3，and VEGF in the PC3 cells in 50 and 100 μg·L^－1IL-17A groups were significantly increased compared 0 and 20 μg·L^－1IL-17A groups （P <0.05）.

Conclusion

IL-17A can obviously promote the migration of prostatic cancer cells， and its mechanism may be related with the activation of IL-17A-stat3-VEGF signaling pathway.

Key words: interleukin-17, signal transducer and activator of transcription 3, vascular endothelial growth factor, prostate cacinoma, metastasis

CLC Number:

R737.25

Zishen XIAO,Shuteng DIAO,Lishuang ZHANG,Jie LIU,Lijuan YANG,Xinyu FENG,Zhenjiang WANG,Yanbo LIU. Promotion effect of IL-17A on migration of prostatic cancer cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2020, 46(6): 1194-1201.

Figures/Tables 7

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References 20

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Group	IL-17A	IL-6	MMP-9	VEGF
Healthy control	3.560 00±0.650 00	0.003 82±0.000 98	0.165 01±0.031 10	0.294 23±0.053 36
Prostate cancer	9.560 00±1.330 00	0.014 35±0.002 55	0.178 00±0.023 00	0.632 12±0.111 32

Group	Migration distance
Group	(t/h) 6	12	24
Control	0.55±0.06	1.02±0.08	1.56±0.16
Experiment	1.96±0.13	2.60±0.36	3.04±0.06
P	<0.05	<0.05	<0.01

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Promotion effect of IL-17A on migration of prostatic cancer cells and its mechanism

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