内蒙古部分地区草原革蜱携带斑点热群立克次体DNA检测及其基因型分布

doi:10.13481/j.1671-587x.20210129

Abstract

Abstract:

Objective： To investigate the status of spotted fever group Rickettsia （SFGR） carried by Dermacentor nuttalli in partial areas of in Inner Mongolia， and to analyze the species of tick-carried SFGR in this area and perform the homology analysis.

Methods

In the middle of April 2019， 264 Dermacentor nuttallis from 708 sheep were collected from Chengchuan Town， early Banner of Etoke Banner， Erdos City； Siziwang Banner， Hohhot City and Bayan WenduSumu area， Arukorqin Banner， Chifeng City， Inner Mongolia. After DNA extraction of single tick， Rickettsia 16sRNA was amplified by PCR method as a preliminary screening experiment， ten positive samples were randomly selected from each region， and a total of 30 positive samples were further amplified for gltA and ompA genes， then the positive samples were sequenced and cluster analysis was performed.

Results

Among 264 ticks， 218 were positive SFGR， with the positive rate of 82.57%； 14 SFGR ompA positive samples and 7 SFGR gltA positive samples were sequenced successfully. The similarities of gltA gene and ompA gene were 100% and 99.86%. The results of phylogenetic analysis showed that the detected sequence was in the same branch with Rickettsia raoultii， and the gltA gene sequence was closely related to Candidatus Rickettsia uralica， Rickettsia parkeri and Rickettsia sibirica， while the ompA gene sequence was close to that of Rickettsia massiliae and Rickettsia rhipicephali； both of them were far away from Rickettsia monacensis.

Conclusion

The genotype of SFGR carried by Dermacentor nuttalli in Ordos， Siziwang Banner and Chifeng， Inner Mongolia is Rickettsia raoultii.

Key words: polymerase chain reaction, Dermacentor nuttalli, Inner Mongolia Strain, Rickettsia raoultii, genotype

CLC Number:

R376

Zheng GUI,Jingfeng YU,Lan MU. Detection of DNA in spotted fever group Rickettsia carried by Dermacentor nuttalli in partial areas of Inner Mongolia and its distribution of genotypes[J].Journal of Jilin University(Medicine Edition), 2021, 47(1): 210-215.

Figures/Tables 5

Tab.1

Fig.1

Fig. 2

Fig. 3

Fig. 4

References 21

1	GUO W P， WANG Y H， LU Q Z， et al. Molecular detection of spotted fever group Rickettsiae in hard ticks， Northern China［J］. Transbound Emerg Dis， 2019， 66（4）： 1587-1596.
2	SATJANADUMRONG J， ROBINSON M T， HUGHES T， et al. Distribution and ecological drivers of spotted fever group rickettsia in Asia［J］. Ecohealth， 2019， 16（4）： 611-626.
3	樊媛媛.草原革蜱Dermacentor nuttalli中Rickettsia的检测及其对宿主遗传多样性的影响［D］.石家庄：河北师范大学，2019：1-59.
4	WANG F， WANG D， GUO G， et al. Species delimitation of the Dermacentor ticks based on phylogenetic clustering and niche modeling［J］. Peer J， 2019， 7：e6911.
5	LIU H， LIANG X T， WANG H J， et al. Molecular evidence of the spotted fever group Rickettsiae in ticks from Yunnan Province， Southwest China［J］. Exp Appl Acarol， 2020， 80（3）： 339-348.
6	BOLDBAATAR B， JIANG R R， FRICKEN M E VON， et al. Distribution and molecular characteristics of Rickettsiae found in ticks across Central Mongolia［J］. Parasit Vectors， 2017， 10（1）： 61.
7	SONG S N， CHEN C F， YANG M H， et al. Diversity of Rickettsia species in border regions of northwestern China［J］. Parasit Vectors， 2018， 11（1）： 634.
8	YIN X H，GUO S C，DING C L， et al. Spotted fever group rickettsiae in Inner Mongolia， China，2015－2016［J］. Emerg Infect Dis， 2018， 24（11）： 2105-2107.
9	MENDELL N L， REYNOLDS E S， BLANTON L S， et al. Detection of Rickettsiae， Borreliae， and Ehrlichiae in ticks collected from Walker County， Texas， 2017-2018［J］. Insects， 2019， 10（10）：E315.
10	LI K， STANOJEVIC M， STAMENKOVIC G， et al. Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia［J］. Sci Rep， 2019， 9（1）： 18680.
11	MIRANDA J， MATTAR S. Molecular detection of Rickettsia bellii and Rickettsia sp. strain Colombianensi in ticks from Cordoba， Colombia［J］. Ticks Tick Borne Dis， 2014， 5（2）： 208-212.
12	ROUX V，RYDKINA E，EREMEEVA M， et al. Citrate synthase gene comparison， a new tool for phylogenetic analysis， and its application for theRickettsiae［J］. Int J Syst Bacteriol， 1997， 47（2）： 252-261.
13	冯　立，王　卓，杨军，等.黑龙江省逊克地区森林革蜱斑点热立克次体DNA的检测［J］.中国人兽共患病学报，2014，30（10）：1020-1023.
14	ZHANG G S， ZHENG D， TIAN Y Q， et al. A dataset of distribution and diversity of ticks in China［J］.Sci Data， 2019， 6（1）： 105.
15	IGOLKINA Y， KRASNOVA E， RAR V， et al. Detection of causative agents of tick-borne Ｒickettsioses in Western Siberia， Russia： identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples［J］. Clin Microbiol Infect， 2018， 24（2）： 199.e9-199.e12.
16	ZHAO S， YANG M H， JIANG M M， et al. Rickettsia raoultii and Rickettsia sibirica in ticks from the long-tailed ground squirrel near the China-Kazakhstan border［J］. Exp Appl Acarol， 2019， 77（3）： 425-433.
17	LU M， LI F， LIAO Y， et al. Epidemiology and diversity of rickettsiales bacteria in humans and animals in Jiangsu and Jiangxi provinces， China［J］. Sci Rep， 2019， 9（1）：13176.
18	程成，鞠文东，王艳梅，等.黑龙江口岸蜱携带斑点热群立克次体及嗜吞噬细胞无形体复合感染调查［J］.口岸卫生控制，2019，24（2）：53-59.
19	闻静，焦丹，鞠文东，等.新发蜱传病原体：劳氏立克次体的研究现状［J］.中国人兽共患病学报，2015，31（4）：361-364.
20	周磊，汤芳，栾进，等.内蒙古奇乾地区蜱携带斑点热立克次体的调查［J］.中国国境卫生检疫杂志，2017，40（2）：96-99.
21	FAN M Y， WALKER D H， LIU Q H， et al. rickettsial and serologic evidence for prevalent spotted fever Rickettsiosisin Inner Mongolia［J］. Am J Trop Med Hyg， 1987， 36（3）： 615-620.

[1]	ZHANG Lifang, CAI Lin, WANG Yunhua, ZHANG Keshi, WU Rina. Analysis on association between selenoprotein P polymorphism and risk of papillary thyriod carcinoma [J]. Journal of Jilin University(Medicine Edition), 2020, 46(02): 383-388.
[2]	RAN Nan, MA Mingxing, PANG Zhiqiang, WANG Zeyu, LIU Yue, ZHENG Ruipeng, LU Junying, ZHANG Chao, CHEN Guang, ZHANG Hong, WANG Fang. Development and evaluation of thrombosis susceptibility gene chip [J]. Journal of Jilin University(Medicine Edition), 2020, 46(01): 182-187.
[3]	GAO Lijun, HE Chengyuan, LI Yingnuo, BA Hongyu, LI Zitong, XIA Wei, LI Mingcheng, YUAN Guanxin, ZHANG Lihua, AI Jinxia. Characteristics and identification of DNA fingerprint of velvet antler and its counterfeits based on duplex PCR technique [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 839-844.
[4]	WANG Mingrui, ZHAI Xiuyu, WANG Gang, ZHANG Wenlan, GAO Baoshan, ZHOU Honglan, FU Yaowen. Effects of CYP3A5 genetic polymorphisms on metabolism of tacrolimus in patients after kidney transplantation [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 537-542.
[5]	XING Shaoji, ZHENG Liansheng, WANG Diandong, ZHANG Xuan, CHI Min. Analysis on association between single nucleotide polymorphism of selenoprotein S gene and risk of liver cancer [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 558-562.
[6]	HUANG Baoliang, DING Chuanbo, WANG Jiaqi, ZHENG Yinan, LIU Wencong, ZHANG Jing, XU Xiaohua. Anti-fatigue effect of arginylfructosylglucose from red ginseng in mice [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 897-902.
[7]	YANG Zhujun, HOU Xu, YU Wenwen, YAN Kuo, SHEN Yuqin, SUN Xinhua. Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats [J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 752-756.
[8]	WEN Aiping, WANG Haijiao, JIN Jingji, CAI Yong, ZHU Jihong, CHEN Lunhua. Expressions of hMOFprotein and mRNA in endometrial cancer tissue and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1138-1142.
[9]	LIU Huanxin, ZHANG Guoxiang, GUO Linlang, TAN Songshan. Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 942-948.
[10]	DONG Zefeng, ZHENG Tianli, FAN Xuejia, Pei Xiaofang. Epidemic characteristics of rhinovirus in hospitalized children with respiratory syndrome in Chengdu during 2011-2013 [J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 177-181.
[11]	LIU Yang, CHEN Kelin, LI Xiangyan, LI Qing, LI Jinghua. Detection of human papillomavirus DNA genotypes in different populations and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1211-1214.
[12]	FU Qianxi, ZHOU Li, TANG Min, ZHANG Mingjun, TANG Xiaojun. Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 808-813.
[13]	SUN Xiaohong, FANG Fuyuan, LI Hui, YIN Na, PANG Zuoliang, LI Huiwu. Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 814-819.
[14]	PENG Jing, YUAN Ruili, WANG Xiaoqin, WU Feng, GUO Xuan. Detection of expression levels of microRNA-181a and microRNA-20a in serum of patients with multiple myeloma and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 625-630.
[15]	ZHAO Min-xue，LI Bai-wen，ZHU Lin，YE Hong-shan，TIAN Xiao-feng，WEI Liang，CAO Hong. Detection of miR-21 expression levels in carcinoma tissue and serum of patients with papillary thyroid carcinoma and its significance [J]. Journal of Jilin University Medicine Edition, 2014, 40(06): 1237-1240.

Detection of DNA in spotted fever group Rickettsia carried by Dermacentor nuttalli in partial areas of Inner Mongolia and its distribution of genotypes

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 21

Related Articles 15

Metrics

Comments

Recommended 10