Abstract:

Abstract:Objective
To explore the effect of Schisandra chinensis extract on neurospheres of glioma and to study the possible mechanism of inhibition.Methods Glioma SHG-44 cells were cultured in stem cells media (containing neurobasal media supplemented with B27,EGF and bFGF) and the neuroshperes were used in this experiment after 3 passages.Neurospheres were cultured and divided into control,50,100 and 200 mg•L^-1  Schisandra chinensis extract groups.The influence of Schisandra chinensis extract in proliferation of neuroshperes was measured by MTT assay.The levels of Bcl-2,Bax and caspase-3 proteins were measured by ELISA. Results  Compared with control group,the inhibitory rates of proliferation of neurospheres in 50,100 and 200 mg•L^-1  Schisandra chinensis extract groups were significantly decreased after treated for 24,48 and 72 h (P<0.01). The ELISA results showed that compared with control group,the secretion of Bcl-2 and Bax protein was decreased,especially the levels of Bcl-2 protein in 100 and 200 mg•L^-1  Schisandra chinensis extract groups (P<0.05 or P<0.01);compared with control group,the ratios of Bax/Bcl-2 in 50,100 and 200 mg•L^-1  Schisandra chinensis extract groups were increased (P<0.01).Compared with respective control group,the expression of caspase-3 protein in 50 mg•L^-1  group was significantly increased (P<0.01).Conclusion Schisandra chinensis extracts might up-regulate the ratio of Bax/Bcl-2 and promote the secretion of apoptosis factors to induce the apoptosis and inhibit the growth of glioma neuroshperes.

Key words: Schisandra chinensis, glioma, neuroshpere, bax protein, bcl-2 protein, caspase-3 protein