Abstract:

To discuss the protective effect of different concentrations of Bilberry anthocyanidin on microwave radiation damage of vascular endothelial cells and to clarify the application value of anthocyanidin in radiation injury.Methods The anthocyanidin was extracted by the method of microwave-assisted.The EVC 304 cells were cultivated in vitro.The cells were divided into control group,20 mW•cm-2 microwave radiation group and 25,50,and 100 mg•L-1 anthocyanidin group.The changes of the cell morphology were observed by inverted microscope,the inhibitory rate of proliferation was detected by MTT method,the cell cycle and apoptotic rate were detected by flow cytometry,and the activity of caspase-3 was assayed.Results The cell morphology in microwave radiation group showed contraction,rounded,smaller size,cell gap widening,and most of the cells showed breaking phenomenon.Compared with microwave radiation group,the morphology of cells  in  anthocyanidin group was significantly improved.The MTT experiment result showed the inhibitory rate of  proliferation of EVC 304 cells reached 58.6% after  20 mW?cm-2 microwave radiation for 24 h  and the inhibitory rate of proliferation  of cells in anthocyanidin group was 30.5%,which was lower than that in  microwave radiation group (P<0.05).The flow cytometry results showed that the apoptotic rate of the  cells  in control group was 2.9%;25,50,and 100 mg?L-1 anthocyanidin could  reduce the apoptotic rate of the cells in  microwave group from 14.9% to 6.1%,4.2%,and 3.5%.Compared with  microwave radiation group,the activity of caspase-3 of the cells in microwave radiation group was higher than that in control group(P<0.05). 25,50, and 100 mg?L-1 anthocyanidin could reduce the activity of caspaase-3,which was lower than that microwave radiation group (P<0.05).Conclusion Bilberry anthocyanidin has protective effect on
the radiation damage of vascular endothelial cells.

Key words: Bilberry anthocyanidin, radiation damage, apoptosis