Abstract:

Abstract:Objective To study the effect of the mitochondrial oxidative lesion in the pathologic mechanism of vascular dementia(VD) in rats,and provide theoretical basis for treatment of VD.Methods The VD rat model through ligating bilateral occlusion of both common carotid arteries was made.The rats ware divided into sham group,ischemia-1-month group,and ischemia-2-month group.The pathologic   and histologic changes of rats after Morris Water Maze and place navigation were determined.The SOD activity and  the MDA content were measured by UV-spectrophotometer and the fluidity of mitochondrial membrane was detected by fluorescence spectrophotometer.
Results Compared with sham group,the escape latencies in ischemia-1-month and ischemia-2-month group were prolonged significantly(P＜0.05)，the SOD activity was decreased significantly(P＜0.05),the MDA content and the viscous coefficient (η) of mitochondrial membrane were significantly increased(P＜0.05).
Conclusion The pathologic mechanism in VD rats is concerned with the metabolism of SOD and MDA and the fluidity of mitochondrial membrane,and is  related to the mitochondrial oxidative stress.Above all,mitochondrial oxidative lesion may be one of pathologic mechanism of VD.

Key words: vascular dementia, superoxide dismutase;malondialdehyde;mitochondrion membrane , fluidity